A Retrospective Analysis of Bacteriological Studies of Surgical Site Infections in a Monoprofile, Multidepartmental Orthopedic Hospital

Grajek, Aleksandra; Białecki, Jerzy; Marczyński, Wojciech; Walczak, Piotr; Macias, Julia

doi:10.5604/15093492.1162427

Cited by 1 publication

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“…P. aeruginosa, ATCC 27,853; and 4. E. coli, ATCC 25,922 (Grajek et al 2015). The bacterial species and growth media are presented in Table 1.…”

Section: Methodsmentioning

confidence: 99%

“…This might indicate that the parameters of sonication were inappropriate for the bacteria causing PJI. According to a retrospective analysis of surgical site infections (SSI) at the current institution, the most common pathogens responsible for PJI are Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Enterococcus faecalis (E. faecalis), Escherichia coli (E. coli), and Pseudomonas aeruginosa (P. aeruginosa) (Grajek et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Ultrasound frequency of sonication applied in microbiological diagnostics has a major impact on viability of bacteria causing PJI

Dudek

Grajek

Kowalczewski

et al. 2020

International Journal of Infectious Diseases

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Sonication of explanted prosthesis constitutes an element of microbiological diagnostics. The aim of performing this procedure is to remove biofilm and to increase sensitivity of diagnostics. Ultrasound used for medical purposes are low-frequency and low-intensity. With this wide range of frequency which can be used in sonication process it is necessary to find the golden mean between biofilm dislodging and planktonic bacteria sparing. Materials and methods: The aim of this study was to determine the least harming low-intensity ultrasound frequency (35 kHz, 40 kHz or 53 kHz) used during sonication process with other parameters constant. Four bacteria species were examined: S. aureus, E. faecalis, E. coli, K. pneumoniae. Number of microbiological studies (n) for each group (g) counted 40 specimens (based on scheme 1 bacteria type -4 groups, 40 studies each). Results: A detailed analysis of gathered data was conducted. Based on study findings following conclusions were drawn. Sonication has a significant and negative impact on survival of sonicated planktonic bacteria. Part of bacteria in planktonic state are damaged/killed by ultrasound, which is demonstrated by lower CFU count in sonicated samples versus control group. Conclusions: Optimal ultrasound frequencies for sonication of S. aureus, P. aeruginosa and E. coli are 35 kHz and 40 kHz. Ultrasound frequencies used in sonication process (35 kHz, 40 kHz, 53 kHz) of E. coli showed same impact on bacteria survival. It is crucial to perform further assessment of ultrasound parameters on clinical effects of sonication used in PJI diagnostics.

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“…P. aeruginosa, ATCC 27,853; and 4. E. coli, ATCC 25,922 (Grajek et al 2015). The bacterial species and growth media are presented in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ultrasound frequency of sonication applied in microbiological diagnostics has a major impact on viability of bacteria causing PJI

Dudek

Grajek

Kowalczewski

et al. 2020

International Journal of Infectious Diseases

Self Cite

View full text Add to dashboard Cite

show abstract

A Retrospective Analysis of Bacteriological Studies of Surgical Site Infections in a Monoprofile, Multidepartmental Orthopedic Hospital

Cited by 1 publication

References 16 publications

Ultrasound frequency of sonication applied in microbiological diagnostics has a major impact on viability of bacteria causing PJI

Ultrasound frequency of sonication applied in microbiological diagnostics has a major impact on viability of bacteria causing PJI

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