A Retrievable and Highly Selective Fluorescent Probe for Monitoring Sulfide and Imaging in Living Cells

Abstract

A novel selective fluorescent chemosensor based on an 8-hydroxyquinoline-appended fluorescein derivative (L1) was synthesized and characterized. Once combined with Cu(2+), it displayed high specificity for sulfide anion. Among the various anions, only sulfide anion induced the revival of fluoresecence of L1, which was quenched by Cu(2+), resulting in "off-on"-type sensing of sulfide anion. What's more, the sensor was retrievable to indicate sulfide anions with Cu(2+), and S(2-), in turn, increased. With the addition of Cu(2+), compound L1 could give rise to a visible pink-to-yellow color change and green fluorescence quenching. The resulting yellow solution could change to pink and regenerate to green fluorescence immediately upon the addition of sulfide anion; however, no changes were observed in the presence of other anions, including CN(-), P(2)O(7)(4-), and other forms of sulfate, making compound L1 an extremely selective and efficient sulfide chemosensor. The signal transduction occurs via reversible formation-separation of complex L1Cu and CuS. What's more, the biological imaging study has demonstrated that the chemosensor can detect sulfur anions in biological systems at a relatively low concentration.

A Copper Porphyrin for Sensing H2S in Aqueous Solution via a “Coordinative‐Based” Approach

“…The resulting benzene solution is evaporated to dryness under vacuum to yield the purple-brown solid, yield 84 %. C 34 Absorbance and Fluorescence Measurements: Absorption spectra were recorded with a Cary-50 spectrophotometer, using a 1 cm quartz cuvette (Hellma Benelux bv, Rijswijk, Netherlands) and a slit-width equivalent to a bandwidth of 5 nm. Fluorescence spectra were measured with a Cary Eclipse spectrophotometer in a 10 ϫ 10 mm 2 airtight quartz fluorescence cuvette (Hellma Benelux bv, Rijswijk, Netherlands) with an emission band-pass of 10 nm and an excitation band-pass of 5 nm.…”

“…generally produce fluorescence turn-on changes via H 2 Smediated precipitation of Mt-S. [1,[32][33][34][39][40][41][42] Despite all the advantages of the above mentioned probes (e.g. high selectivity and sensitivity), some shortcomings typically involve slow response and poor water solubility.…”

Design of a novel coumarin-based multifunctional fluorescent probe for Zn2+/Cu2+/S2− in aqueous solution

“…We presumed that the 'in situ' prepared L-Cu exhibited a selective response to S 2 − based on simple inorganic-reaction. And, in short, firstly, the fluorescence of HL was quenched by Cu 2+ , and then Cu 2+ would be dragged out from the complex by S 2− which was known to react with Cu 2+ to make a very stable CuS (K sp = 6.3 × 10 −36 ), as a result, the quenched fluorescence recurred remarkably [50][51][52][53][54] (Scheme 3). It was further confirmed by the UV-vis spectra of reversible change [55][56][57].…”

Carbon dots: synthetic methods and applications as fluorescent probes for the detection of metal ions, inorganic anions and organic molecules

“…17 The quenching of CDs fluorescence by Hg 2+ , fluorescence recovery by iodide addition manufacturing. But S 2− is also a toxic pollutant for the environment [87]. Thus, it is highly desirable to develop a sensitive method for instant S 2− monitoring.…”