A resource of targeted mutant mouse lines for 5,061 genes

Abstract

The International Mouse Phenotyping Consortium reports the generation of new mouse mutant strains for over 5,000 genes from targeted embryonic stem cells on the C57BL/6N genetic background. This includes 2,850 null alleles for which no equivalent mutant mouse line exists, 2,987 novel conditional-ready alleles, and 4,433 novel reporter alleles. This nearly triples the number of genes with reporter alleles and almost doubles the number of conditional alleles available to the scientific community. When combined with more than 30 years of community effort, the total mutant allele mouse resource covers more than half of the genome. The extensively validated collection is archived and distributed through public repositories, facilitating availability to the worldwide biomedical research community, and expanding our understanding of gene function and human disease.

Genes adapt to outsmart gene targeting strategies in mutant mouse strains by skipping exons to reinitiate transcription and translation

“…We also analyzed 3,674 conditional-ready mouse strains [32] using the incomplete CDS approach (Supplementary Table 6), and determined that at least 40% of conditional-ready alleles can skew the mouse phenotype (Fig. 5d).…”

CLIC4 Regulates Endothelial Barrier Control by Mediating PAR1 Signaling via RhoA

“…All animal studies and experiments were approved by the Institutional Animal Care and Use Committee of the University of Illinois. Clic4 tm1a(EUCOMM)HMGU targeted embryonic stem cells were obtained from the European Conditional Mouse Mutagenesis Program 33 and were injected into blastocysts for chimera production at the Transgenic & Targeted Mutagenesis Laboratory at Northwestern University. Two chimeric males were generated and transferred to our animal facility at the University of Illinois at Chicago Biologic Resources Laboratory.…”

LAMA: automated image analysis for the developmental phenotyping of mouse embryos

“…All animal studies were licensed by the Home Office under the Animals (Scientific Procedures) Act 1986 Amendment Regulations 2012 (SI 4 2012/3039), UK, and additionally approved by the Institutional Ethical Review Committee. The Acan tm1b allele was obtained by cre deletion of C57BL/6N-Acan tm1a(EUCOMM)Hmgu /H (EM:10224) mice as described previously ( Birling et al, 2019 preprint). Homozygous mutants are named Acan −/− here.…”

“…Review Committee. The Acan tm1b allele was obtained by cre deletion of C57BL/6N-Acan tm1a(EUCOMM)Hmgu /H (EM:10224) mice as described in (Birling et al, 2019). Homozygous mutants are named Acan -/here.…”