A resorbable nerve conduit as an alternative to nerve autograft in nerve gap repair

Hazari, Anita; Wiberg, Mikael; Johansson-Rudén, G.; Green, C.; Terenghi, Giorgio

doi:10.1054/bjps.1999.3184

Cited by 113 publications

(69 citation statements)

References 17 publications

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“…PHB has been successfully used as a conduit material to bridge a gap in the rat sciatic nerve (Hazari et al, 1999). However, empty PHB conduits are not sufficient to support adequate nerve regeneration and allow full functional recovery.…”

Section: Discussionmentioning

confidence: 99%

“…Using an operating microscope (Zeiss, Germany), a resorbable polyhydroxy butyrate (PHB) conduit (Astra Tech, Sweden) was grafted in adult male Lewis rats (Harlan), average weight of 180 g, to bridge a gap of 1 cm in the left sciatic nerve (Hazari et al, 1999). There were four experimental groups (n ϭ 6 in each group) that were grafted with a PHB conduit containing 20, 40, 80, or 160 ϫ 10 6 cells/ml and the control group (n ϭ 6) was implanted with PHB conduits without SCs.…”

Section: In Vivo Rat Sc Transplantationmentioning

confidence: 99%

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Retroviral labeling of Schwann cells: In vitro characterization and in vivo transplantation to improve peripheral nerve regeneration

Mosahebi

Woodward

Wiberg

et al. 2001

Glia

148

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Transplantation of Schwann cells (SCs) is a promising treatment modality to improve neuronal regeneration. Identification of the transplanted cells is an important step when studying the development of this method. Genetic labeling is the most stable and reliable method of cell identification, but it is still unclear whether it has deleterious effect on SC characteristics. Our aim was to achieve a stable population of SCs transduced with the lacZ gene at a high frequency using a retroviral vector in vitro, and to follow the labeled SC in vitro to assess their viability and phenotypic marker expression. Furthermore, we transplanted lacZ-labeled SCs in a conduit to repair peripheral nerve to investigate their effect on nerve regeneration in vivo. Rat and human SCs were cultured and transduced with an MFG lacZ nls marker gene, achieving a transduction rate of 80% and 70%, respectively. Rat SCs were kept in culture for 27 weeks and examined every 4 weeks for expression of lacZ, viability, and phenotypic marker expression of GFAP, p75, MHC I and II. Throughout this period, transduced rat SCs remained viable and continued to proliferate. The proportion of cells expressing lacZ dropped only by 10% and the expression of phenotypic markers remained stable. Transduced human SCs were followed up for 4 weeks in culture. They proliferated and continued to express the lacZ gene and phenotypic marker expression of GFAP and p75 was preserved. Primary culture of transduced rat SCs were transplanted, syngeneically, in a conduit to bridge a 10 mm gap in sciatic nerve and the grafts were examined after 3 weeks for the presence and participation of labeled SCs and for axonal regeneration distance. Transplanted transduced rat SCs were clearly identified, taking part in the regeneration process and enhancing the axonal regeneration rate by 100% (at the optimal concentration) compared to conduits without SCs. Thus, retroviral introduction of lacZ gene has no deleterious effect on SCs in vitro and these SCs take part and enhance nerve regeneration in vivo. GLIA 34:8-17, 2001.

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Section: Discussionmentioning

confidence: 99%

Section: In Vivo Rat Sc Transplantationmentioning

confidence: 99%

Retroviral labeling of Schwann cells: In vitro characterization and in vivo transplantation to improve peripheral nerve regeneration

Mosahebi

Woodward

Wiberg

et al. 2001

Glia

148

View full text Add to dashboard Cite

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“…In many cases in vivo work has been carried out using P(3HB) to regenerate nerve tissues, for example P(3HB) has been used to repair transected superficial radial nerves in cats for an experimental period of 12 months which showed normal inflammatory response and axonal regeneration of 2-3 mm nerve gap [92,93]. While another experiment on rat sciatic nerve showed axonal regeneration of 10 mm nerve gap with low inflammation [92,93].…”

Section: Nerve Tissue Engineeringmentioning

confidence: 99%

Polyhydroxyalkanoates: The Natural Polymers Produced by Bacterial Fermentation

Panchal

Bagdadi

Roy

2012

Advances in Natural Polymers

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“…This polymer has a very high Young's modulus of approximately 4 GPa [64] and has only recently been used in nerve conduits. [65,66] PCL is a semicrystalline synthetic copolymer currently being favored for the fabrication of nerve conduits. Like other synthetic biopolymers, it is degraded by acid-hydrolysis of the ester bond, releasing acidic monomers.…”

Section: Tubular Scaffolds To Guide Nerve Growthmentioning

confidence: 99%

Tissue Engineering Strategies Designed to Realize the Endogenous Regenerative Potential of Peripheral Nerves

et al. 2009

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http://doi.wiley.com/10.1002/adma.v21:32/33 Bridging peripheral nerve gaps without the use of autografts has significant clinical importance. But in order to rationally design novel scaffolds, a good understanding of the nerve regeneration process is vital. Appropriate amount of structural and chemical cues are required to stimulate the endogenous mechanisms of repair and functional recovery. Synthetic and natural materials present various opportunities to induce the growth of supporting cells as well as promote axon regeneration. An overview of tissue engineering strategies currently being explored that stimulate the different steps of the regenerative sequence is presented.

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A resorbable nerve conduit as an alternative to nerve autograft in nerve gap repair

Cited by 113 publications

References 17 publications

Retroviral labeling of Schwann cells: In vitro characterization and in vivo transplantation to improve peripheral nerve regeneration

Retroviral labeling of Schwann cells: In vitro characterization and in vivo transplantation to improve peripheral nerve regeneration

Polyhydroxyalkanoates: The Natural Polymers Produced by Bacterial Fermentation

Tissue Engineering Strategies Designed to Realize the Endogenous Regenerative Potential of Peripheral Nerves

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