A resonance tracking method for stable operation of a near-field scanning optical microscope in liquid environment

Park, Doo Jae; Park, Kyoung Duck; Choi, Geun Chang; Lee, Seung Gol; Byeon, Clare Chisu; Jeong, Mun Seok; Choi, Soo Bong

doi:10.1016/j.cap.2013.11.050

Cited by 3 publications

(1 citation statement)

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“…In addition to the high Q -factor, maintaining a stable resonance condition under aqueous conditions is also significant. Recently, we used the stable resonance zone of fiber probe and the resonance tracking method to overcome the resonance change issue when the immersion depth of the fiber probe is changed due to the evaporating liquid. , Despite these methods being useful for small area scanning, they are inappropriate to apply to large samples such as biological cells. Thus, we adopt the diving bell structure to maintain a stable resonance condition regardless of liquid evaporation and scanning time …”

Section: Resultsmentioning

confidence: 99%

Near-Field Imaging of Cell Membranes in Liquid Enabled by Active Scanning Probe Mechanical Resonance Control

Park

Raschke

Jang³

et al. 2016

J. Phys. Chem. C

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Despite the power of far-field super-resolution microscopies for three-dimensional imaging of biomolecular structures and processes, its application is challenged in dense and crowded samples and for certain surface and membrane studies. Although near-field imaging with its ability to provide intrinsic subdiffraction limited spatial resolution at any optical modality, its application to biological systems has remained limited because of the difficulties of routine operation in liquid environments. Here we demonstrate stable and sensitive near-field scanning optical microscopy (NSOM) in a liquid based on a new mechanical resonance control and an optimization of the tip length, achieving a high quality factor (>2800) force sensing of the near-field probe. Through near-field imaging of the spatial distribution of epidermal growth factor receptors (EGFRs) on the membrane of A431 cancer cells as an example, we reveal nanoscale correlations between surface EGFR and intracellular organelle structures with ∼50 nm spatial resolution. The method provides a new avenue for surface imaging in viscous liquid media to complement super-resolution microscopy for studies of biological membranes, nanostructures, and interfaces.

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Section: Resultsmentioning

confidence: 99%