A resistance gene against potato late blight originating from Solanum × michoacanum maps to potato chromosome VII

Abstract

Solanum × michoacanum (Bitter.) Rydb. is a diploid, 1 EBN (Endosperm Balance Number) nothospecies, a relative of potato originating from the area of Morelia in Michoacán State of Mexico that is believed to be a natural hybrid of S. bulbocastanum × S. pinnatisectum. Both parental species and S. michoacanum have been described as sources of resistance to Phytophthora infestans (Mont.) de Bary. The gene for resistance to potato late blight, Rpi-mch1, originating from S. michoacanum was mapped to the chromosome VII of the potato genome. It confers high level of resistance since the plants possessing it showed only small necrotic lesions or no symptoms of the P. infestans infection and we could ascribe over 80% of variance observed in the late blight resistance test of the mapping population to the effect of the closest marker. Its localization on chromosome VII may correspond to the localization of the Rpi1 gene from S. pinnatisectum. When mapping Rpi-mch1, one of the first genetic maps made of 798 Diversity Array Technology (DArT) markers of a plant species from the Solanum genus and the first map of S. michoacanum, a 1EBN potato species was constructed. Particular chromosomes were identified using 48 sequence-specific PCR markers, originating mostly from the Tomato-EXPEN 2000 linkage map (SGN), but also from other sources. Recently, the first DArT linkage map of 2 EBN species Solanumphureja has been published and it shares 197 DArT markers with map obtained in this study, 88% of which are in the concordant positions.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-011-1715-4) contains supplementary material, which is available to authorized users.

Genetic composition of interspecific potato somatic hybrids and autofused 4x plants evaluated by DArT and cytoplasmic DNA markers

“…Clones mch /8 and mch /39 were derived from mch [accession VIR5763 from the N. I. Vavilov Research Institute of Plant Industry (VIR) potato collection (Zoteyeva et al 2012)] and both were resistant to P. infestans . The mch /8 was the parental form of mapping population used for locating the gene for resistance to P. infestans , Rpi - mch1, to the potato chromosome VII (Śliwka et al 2012a). The CAPS marker C2_At1g53670 was the closest to the Rpi - mch1 gene (located 5.7 cM from it), and was used as a diagnostic marker among six somatic hybrids originating from mch /8 parent.…”

“…The quality of DNA was assessed on 1.5 % agarose gels. The DArT analysis was performed in Diversity Array Pty Ltd. Canberra, Australia, as described for mch and S. ruiz - ceballosii by Śliwka et al (2012a, b) based on protocols for other plant species (Jaccoud et al 2001; Wenzl et al 2004; Akbari et al 2006). To maximize the output of the analysis, samples were processed by two panels: one dedicated to wild potato species and one representing clones of tbr.…”

“…A reduced fraction of the genome is prepared by restriction enzyme digestion of genomic DNA followed by the ligation of restriction fragments to adapters. The combination of Pst I/ Taq I enzymes was selected for potato marker finding (Śliwka et al 2012a). The genome complexity is then reduced by PCR amplification.…”

“…DArT linkage maps of potato were created for Solanum   ×   michoacanum ( mch ) (Śliwka et al 2012a), Solanum ruiz - ceballosii (Śliwka et al 2012b) and a doubled haploid DM1–3 of Solanum phureja , for which a physical map is available, too (Sharma et al 2013). Three additional DArT maps of diploid potato (Sołtys-Kalina et al 2015; Śliwka et al 2016; data unpublished, personal communication with Agnieszka Hara-Skrzypiec) are available.…”

“…Using the DArT method, it is possible to generate several hundred to several thousand markers in a single assay (Kilian et al 2005; Wittenberg et al 2005). For example, 846 DArT markers for mch and 1827 DArT markers for S. phureja have been mapped on genetic maps of potato (Śliwka et al 2012a; Sharma et al 2013). …”