A resilient and flexible chitosan/silk cryogel incorporated Ag/Sr co-doped nanoscale hydroxyapatite for osteoinductivity and antibacterial properties

Li, Pengfei; Jia, Zhanrong; Wang, Qun; Tang, Pengfei; Wang, Menghao; Wang, Kefeng; Fang, Ju; Zhao, Chong; Ren, Fuzeng; Ge, Xiang; Li, Xiong

doi:10.1039/c8tb01672k

Cited by 60 publications

(41 citation statements)

References 48 publications

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“…With respect to the above features, SF porous scaffolds with high porosity, suitable mechanical properties, and biodegradability facilitate cell adhesion, proliferation, and migration as well as transmission of nutrients and wastes. , Although progress has been made recently in improving the pore structure and mechanical properties of these scaffolds, novel methods of generating pores, especially nanoscale pores, without sacrificing mechanical properties are still desired. − Freeze-drying is a commonly used method for preparing SF porous scaffolds ,− where pore size, microstructure, and porosity can be modulated by controlling the freezing temperature, , cooling rate, and concentration of the SF solution used in the process. ,− Post-treatments including rinsing with methanol and water vapor annealing are needed to make the freeze-dried sponges water-insoluble. − Methanol is the lowest-molecular weight alcohol and can rapidly permeate the protein structure to induce the transformation of SF from a random coil to a thermodynamically stable β-sheet structure, resulting in water insolubility of the SF scaffolds. However, methanol-treated scaffolds are relatively stiff and brittle and lack a high elastic modulus, which is critical in many areas of application for these scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Flexible Water-Absorbing Silk-Fibroin Biomaterial Sponges with Unique Pore Structure for Tissue Engineering

Liu

Chen

Wang

et al. 2020

ACS Biomater. Sci. Eng.

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Silk fibroin (SF) scaffolds are widely used in tissue engineering due to their biocompatibility and slow biodegradability. However, the relatively stiff mechanical properties and low permeability of these systems can limit some applications. In this study, a new type of water-stable silk sponge (ASF-PEG-S) was obtained by inducing nanoparticle (50–300 nm in diameter) formation in SF solution by autoclaving followed by freeze-drying and rinsing the dry sponges with low-molecular weight (400 Da) polyethylene glycol (PEG400) to induce SF β-sheet structure formation and thus stability in water. With further extraction, the SF nanoparticles embedded in the sponges were removed, leaving nanopores in the walls of round-shaped micro-size pores. The unique pore structure resulted in enhanced permeability and flexibility of ASF-PEG-S when compared to other types of SF sponges, especially with respect to commonly used methanol-annealed SF sponges. In addition, ASF-PEG-S absorbed water nearly 40 times more than its dry weight, while the methanol-annealed sponges absorbed half this amount. When human fibroblasts were seeded and cultured on ASF-PEG-S versus traditional SF methanol-processed sponges, improved cell encapsulation, distribution, and consistency in growth were observed, suggesting utility in tissue engineering and tissue repair applications in the future.

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Section: Introductionmentioning

confidence: 99%

Flexible Water-Absorbing Silk-Fibroin Biomaterial Sponges with Unique Pore Structure for Tissue Engineering

Liu

Chen

Wang

et al. 2020

ACS Biomater. Sci. Eng.

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“…Given the global threat and increasing influence of antibiotic resistance, there is an urgent demand to explore novel antibacterial strategies other than using antibiotics [[12], [13], [14], [15], [16], [17], [18], [19], [20]]. Recently, using a certain surface topography to provide a more persistent antibacterial solution attracts more and more attention [5,[21], [22], [23], [24], [25], [26]].…”

Section: Introductionmentioning

confidence: 99%

Micro/nano-structured TiO2 surface with dual-functional antibacterial effects for biomedical applications

Ren

Ding

et al. 2019

Bioactive Materials

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Implant-associated infections are generally difficult to cure owing to the bacterial antibiotic resistance which is attributed to the widespread usage of antibiotics. Given the global threat and increasing influence of antibiotic resistance, there is an urgent demand to explore novel antibacterial strategies other than using antibiotics. Recently, using a certain surface topography to provide a more persistent antibacterial solution attracts more and more attention. However, the clinical application of biomimetic nano-pillar array is not satisfactory, mainly because its antibacterial ability against Gram-positive strain is not good enough. Thus, the pillar array should be equipped with other antibacterial agents to fulfill the bacteriostatic and bactericidal requirements of clinical application. Here, we designed a novel model substrate which was a combination of periodic micro/nano-pillar array and TiO2 for basically understanding the topographical bacteriostatic effects of periodic micro/nano-pillar array and the photocatalytic bactericidal activity of TiO2. Such innovation may potentially exert the synergistic effects by integrating the persistent topographical antibacterial activity and the non-invasive X-ray induced photocatalytic antibacterial property of TiO2 to combat against antibiotic-resistant implant-associated infections. First, to separately verify the topographical antibacterial activity of TiO2 periodic micro/nano-pillar array, we systematically investigated its effects on bacterial adhesion, growth, proliferation, and viability in the dark without involving the photocatalysis of TiO2. The pillar array with sub-micron motif size can significantly inhibit the adhesion, growth, and proliferation of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Such antibacterial ability is mainly attributed to a spatial confinement size-effect and limited contact area availability generated by the special topography of pillar array. Moreover, the pillar array is not lethal to S. aureus and E. coli in 24 h. Then, the X-ray induced photocatalytic antibacterial property of TiO2 periodic micro/nano-pillar array in vitro and in vivo will be systematically studied in a future work. This study could shed light on the direction of surface topography design for future medical implants to combat against antibiotic-resistant implant-associated infections without using antibiotics.

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“…Figure demonstrates the live/dead ratio , for Gram-positive [Figure a] and Gram-negative [Figure b] bacterial cells on unpolarized and polarized BG, BG–10 NKN, BG–20 NKN, and BG–30 NKN samples. It has been observed that the live/dead ratio for both types of bacterial cells decreases with addition of the piezoelectric NKN as the secondary phase in BG [Figure ].…”

Section: Results and Discussionmentioning

confidence: 99%

Electro-mechanical and Polarization-Induced Antibacterial Response of 45S5 Bioglass–Sodium Potassium Niobate Piezoelectric Ceramic Composites

Verma

Singh

Kumar

et al. 2020

ACS Biomater. Sci. Eng.

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Besides the excellent osteoconductivity and biocompatibility of 45S5 bioglass (BG), poor mechanical and electrical properties as well as susceptibility toward bacterial adhesion limit its widespread clinical applications. In this context, the present study investigates the effect of addition of piezoelectric sodium potassium niobate (Na0.5K0.5NbO3; NKN) on mechanical, dielectric, and antibacterial response of BG. BG–xNKN (x = 0, 10, 20, and 30 vol%) composites were synthesized at 800 °C for 30 min. The phase analyses using spectral techniques revealed the formation of the composite without any reaction between BG and piezoelectric ceramic NKN. The dielectric and electrical measurements were performed over a wide range of temperature (30–500 °C) and frequency (1 Hz–1 MHz) which suggests that space charge and dipolar polarizations are the dominant polarization mechanisms. The complex impedance analyses suggest that the average activation energies for grain and grain boundary resistances for BG–xNKN (x = 10, 20, and 30 vol%) composites are 0.59, 0.87, 0.94 and 0.76, 0.93, 1.06 eV, respectively. The issue of bacterial infection has been addressed by electrical polarization of the developed composite samples, at 20 kV for 30 min. Statistical analyses reveal that the viability of Gram-positive (S. aureus) and Gram-negative (E. coli) bacterial cells has been reduced significantly on positively and negatively charged BG–NKN composite samples, respectively. The qualitative analyses using the Kirby–Bauer test supports the above findings. Nitro blue tetrazolium and lipid peroxide assays were performed to understand the mechanism of such antibacterial response, which suggested that the combined effect of NKN addition and polarization significantly enhances the superoxide production, which kills the bacterial cells. Overall, incorporation of NKN in BG enhances the mechanical, electrical, and dielectric properties as well as improves the antibacterial response of polarized BG–xNKN composites.

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A resilient and flexible chitosan/silk cryogel incorporated Ag/Sr co-doped nanoscale hydroxyapatite for osteoinductivity and antibacterial properties

Abstract: A resilient and flexible chitosan/silk cryogel incorporated Ag and Sr co-doped hydroxyapatite exhibits good mechanical, antibacterial and osteoinductive properties.

Cited by 60 publications

References 48 publications

Flexible Water-Absorbing Silk-Fibroin Biomaterial Sponges with Unique Pore Structure for Tissue Engineering

Flexible Water-Absorbing Silk-Fibroin Biomaterial Sponges with Unique Pore Structure for Tissue Engineering

Micro/nano-structured TiO2 surface with dual-functional antibacterial effects for biomedical applications

Electro-mechanical and Polarization-Induced Antibacterial Response of 45S5 Bioglass–Sodium Potassium Niobate Piezoelectric Ceramic Composites

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