A report on identification of sequence polymorphism in barcode region of six commercially important Cymbopogon species

Abstract

CYMBOPOGON: is an important member of grass family Poaceae, cultivated for essential oils which have greater medicinal and industrial value. Taxonomic identification of Cymbopogon species is determined mainly by morphological markers, odour of essential oils and concentration of bioactive compounds present in the oil matrices which are highly influenced by environment. Authenticated molecular marker based taxonomical identification is also lacking in the genus; hence effort was made to evaluate potential DNA barcode loci in six commercially important Cymbopogon species for their individual discrimination and authentication at the species level. Four widely used DNA barcoding regions viz., ITS 1 & ITS 2 spacers, matK, psbA-trnH and rbcL were taken for the study. Gene sequences of the same or related genera of the concerned loci were mined from NCBI domain and primers were designed and validated for barcode loci amplification. Out of the four loci studied, sequences from matK and ITS spacer loci revealed 0.46% and 5.64% nucleotide sequence diversity, respectively whereas the other two loci i.e., psbA-trnH and rbcL showed 100% sequence homology. The newly developed primers can be used for barcode loci amplification in the genus Cymbopogon. The identified Single Nucleotide Polymorphisms from the studied sequences may be used as barcodes for the six Cymbopogon species. The information generated can also be utilized for barcode development of the genus by including more number of Cymbopgon species in future.

Genetic Diversity Analysis Revealed the Hot Spot of Acmella Paniculata Existing in Natural Populations of Gujarat

“…The traditional identi cation method becomes less effective for A. paniculata and A. radicans because of similarities in morphological features except for ower color (the white color ower is A. paniculata and the yellow ower colour is A radicans), owering season, and its habitat. Hence we opted for the DNA barcoding technique; which has been proved as a more reliable, authenticate and accurate tool for the identi cation of an organism (Kress et al 2007;Hebert et al 2009;Bishoyi et al 2017;Li et al 2021) Molecular markers are employed to assess genetic diversity which will reveal the distribution and extent of genetic variation within the species (Nybom and Bartish 2000). DNA-based molecular markers are the best choice for accurately assessing genetic diversity among the population (Bishoyi et al 2018;Nadeem et al 2018).…”