Our understanding of the mechanisms underlying mutant genes affecting the limb skeleton is very incomplete. At this stage description of new phenotypes is still valuable, especially when they differ markedly from those previously described and have counterparts in man.
The gene for polysyndactyly (Ps) arose during a neutron irradiation experiment at the M.R.C. Radiobiological Unit at Harwell (Batchelor, Phillips & Searle, 1966), and was offered to the author in November 1964. The mice from Harwell were outcrossed to CB ♀♀ (the F1 of CBA/Gr ♂♂ × C57 BL/Gr ♀♀). The stock was outcrossed again in late 1966 to improve fertility. Rigorous brother/sister mating has not been practised.
Embryos from Ps/ + × Ps/ + matings were fixed in Bouin's solution, sectioned at 7·5 or 10μ according to age and stained with H & E. In most cases only the limbs were sectioned (left fore and hind limbs transversely, right ones tangentially).