A replicative form of the DNA of minute virus of mice

Dobson, Phyllis R.; Helleiner, C. W.

doi:10.1139/m73-005

Cited by 10 publications

(7 citation statements)

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“…The specific S phase dependence of viral DNA synthesis suggests that a homology between viral DNA and host cell DNA may exist and results in a coordination of the initiation of viral DNA synthesis with certain replicons of the host cell. During the preparation of this manuscript a report appeared describing the isolation of RF DNA for MVM virus and evidence for homology between MVM and L-cell DNA (6). Experiments are in progress to determine if H-1 DNA has homology to late replicating hamster embryo DNA.…”

Section: Resultsmentioning

confidence: 99%

Replication Process of the Parvovirus H-1 II. Isolation and Characterization of H-1 Replicative Form DNA

Rhode

1974

J Virol

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The Parvovirus H-1 replicates autonomously in hamster embryo cells. A DNA synthetic event, called HA-DNA synthesis, upon which subsequent viral RNA and viral hemagglutinin synthesis is dependent, is initiated in late S phase of the infected cell (18). It was postulated that HA-DNA represents parental viral replicative form DNA (RF DNA). This study describes the isolation and characterization of H-1 RF DNA as part of the continuing study of the mechanisms and control of DNA replication in the eukaryotic cell. The H-1 RF DNA is a linear duplex molecule containing the viral strand and its complement. The complementary strands of the RF DNA have been separated by equilibrium density gradient centrifugation. The RF DNA has a buoyant density of 1.705 in neutral CsCl and an estimated guanine plus cytosine (GC) content of 45.9%. It has a sedimentation coefficient of 17 S . The calculated molecular weight of 3.7 × 10 6 is twice that of the single-stranded virion DNA. H-1 virions contain DNA that is homogeneous and free of complementary strands.

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Section: Resultsmentioning

confidence: 99%

Replication Process of the Parvovirus H-1 II. Isolation and Characterization of H-1 Replicative Form DNA

Rhode

1974

J Virol

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“…Following infection, the ss viral DNA is converted to a linear, double-stranded (ds) replicative form (RF) DNA molecule (7)(8)(9)(10) by the host replicative machinery, presumably using the 3' hairpin terminus as a primer-template. This duplex RF DNA is then further replicated and acts as template for the synthesis of the viral DNA strand from a proposed unique replication origin at the 3' end of the complementary strand (11,12).…”

mentioning

confidence: 99%

Proteins tightly associated with the termini of replicative form DNA of Kilham rat virus, an autonomous parvovirus.

Wobbe¹,

Mitra²

1985

Proc. Natl. Acad. Sci. U.S.A.

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Revie et al. [Revie, D., Tseng, B. Y., Grafstrom, R. H. & Goulian, M. (1979) Proc. Nail. Acad. Sci. USA 76,[5539][5540][5541][5542][5543] have proposed that the double-stranded replicative form (RF) DNA of the autonomous rodent parvovirus H-1 has protein of 60 kDa covalently bound at its 5' termini. We present evidence that the RF DNA of a similar rodent parvovirus, Kilham rat virus (KRV), also has covalently bound protein. NaDodSO4/polyacrylamide gel electrophoresis of purified, 1251-labeled RF DNA shows that proteins of 68-72, 66, 64, and 55 kDa copurify with the DNA during velocity and equilibrium sedimentation in the presence of detergents and 4 M guanidine HCL. Phenol extraction in the presence of 2-mercaptoethanol removes the 68-to 72-kDa proteins, but the 66-, 64-, and 55-kDa proteins remain tightly, but noncovalently, bound. The latter polypeptides also appear to associate with protease-treated RF DNA when mixed with uninfected cell extract. Following removal of these proteins by electrophoresis in NaDodSO4/agarose gels, two proteins (called RF TP-90 and RF TP-40), of about 90 and 40 kDa, become evident. These remain bound to the DNA and are released only after nuclease digestion of the DNA. These two proteins, apparently not of viral origin, are associated with terminal restriction fragments of the RF DNA and appear to be covalently bound to the 5' termini of both strands.The commonly studied autonomous rodent parvoviruses [H-1, minute virus of mice (MVM), and Kilham rat virus (KRV)] have a number of properties in common. They have =5000 nucleotide-containing linear single-stranded (ss) DNA genomes with short terminal hairpins (1, 2). The DNA is packaged into an icosahedral capsid about 240 A in diameter (3)(4)(5). The viruses replicate only in host cells undergoing DNA synthesis (6) and are presumed to rely largely, if not entirely, on host factors for DNA synthesis, although no reconstituted replication system using purified proteins has been developed to demonstrate this. Because of their dependence on host S phase, autonomous parvoviruses provide a model system for studying cellular DNA replication.Following infection, the ss viral DNA is converted to a linear, double-stranded (ds) replicative form (RF) DNA molecule (7-10) by the host replicative machinery, presumably using the 3' hairpin terminus as a primer-template. This duplex RF DNA is then further replicated and acts as template for the synthesis of the viral DNA strand from a proposed unique replication origin at the 3' end of the complementary strand (11, 12). These observations have led to the proposal of a "rolling hairpin" model for parvovirus replication (13-16).Based on its aberrant electrophoretic mobility in agarose and an increase in its buoyant density in CsCl following protease treatment, most of the intracellular RF DNAs of H-1 and MVM appear to have protein covalently bound at its termini (16,17 Following the indicated labeling periods, RF DNA was purified from infected cells by a modification of the procedure of Lavelle ...

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“…The replication model described above, which explains these data on MVL51 replication, is similar to the replication systems of other single-stranded bacteriophage (8,10) and animal viruses (1). The fact that these diverse single-stranded DNA viruses (all with DNA molecular weights about 2 x 106) follow the same basic replication scheme must reflect the selective advantage of this replication mechanism; a slight modification to the general model has been found for two parvoviruses (the adenoassociated satellite virus and densonucleosis virus) which contain separately encapsidated complementary single DNA strands (reviewed by Tinsley and Longworth,ref.…”

Section: Smentioning

confidence: 52%

“…The progeny DNA is assembled into mature 4X174 particles, which are then released from the infected cell. The same general model seems to apply to the replication of the single-stranded DNA filamentous bacteriophage (8) and to a single-stranded DNA parvovirus (1).…”

mentioning

confidence: 97%

Intracellular Replication of Mycoplasmavirus MVL51

Liss

Maniloff

1974

J Virol

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The intracellular replication of MVL51, a group Li mycoplasmavirus, was investigated. The single-stranded parental DNA was found to enter the cell and become converted to double-stranded DNA. This replicated to yield additional double-stranded DNA molecules. The parental viral DNA was found to leave the replication complex and become associated with large molecular weight DNA not involved with viral replication. Progeny viral DNA formed from the doublestranded DNA and an intracellular accumulation of virus chromosome size DNA was observed. The interpretation of this data and a suggested model for the viral replication are discussed and compared to viral DNA replication models for other single-stranded DNA viruses.

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A replicative form of the DNA of minute virus of mice

Cited by 10 publications

References 0 publications

Replication Process of the Parvovirus H-1 II. Isolation and Characterization of H-1 Replicative Form DNA

Replication Process of the Parvovirus H-1 II. Isolation and Characterization of H-1 Replicative Form DNA

Proteins tightly associated with the termini of replicative form DNA of Kilham rat virus, an autonomous parvovirus.

Intracellular Replication of Mycoplasmavirus MVL51

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