A Reliable Stem Cell Carrier: An Experimental Study in Wistar Rats

Stocchero, Ithamar Nogueira; Lizier, Nelson Foresto; Stelini, Rafael Fantelli; Oliveira, Olivia Capela Grimaldi; Oliveira, Paulo Roberto Grimaldi; Ayoub, Carlos Alexandre; Rotta, Tainah Desuó; Stocchero, Guilherme Flosi; Kharmandayan, Paulo

doi:10.1007/s00266-019-01469-4

Cited by 4 publications

(4 citation statements)

References 31 publications

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“…Studies on the transplantation of BMMSCs and UCMSCs for spinal cord injury, myocardial ischemia, liver failure, and diabetes all showed significant differences in various aspects of cell behavior such as cell migration [ 41 , 47 , 48 ]. As a result, the degrees of tissue repair and functional recovery are different, which may be related to cell number, survival time, cell activity, and the microenvironment [ 21 , 49 ]. Previous studies have reported that MSCs can be transplanted by intrauterine infusion, as well as by intravenous injection and direct intrauterine transplantation [ 42 , 44 , 49 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

“…As a result, the degrees of tissue repair and functional recovery are different, which may be related to cell number, survival time, cell activity, and the microenvironment [ 21 , 49 ]. Previous studies have reported that MSCs can be transplanted by intrauterine infusion, as well as by intravenous injection and direct intrauterine transplantation [ 42 , 44 , 49 , 50 ]. From the perspective of clinical application, because of the contraction of uterus, intrauterine infusion leads to cells easily flowing out of the uterine cavity.…”

Section: Discussionmentioning

confidence: 99%

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Human Acellular Amniotic Matrix with Previously Seeded Umbilical Cord Mesenchymal Stem Cells Restores Endometrial Function in a Rat Model of Injury

Wang

Shi

Cai

et al. 2021

Mediators of Inflammation

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Background. Abnormal endometrial repair after injury results in the formation of intrauterine adhesions (IUA) and a thin endometrium, which are key causes for implantation failure and infertility. Stem cell transplantation offers a potential alternative for some cases of severe Asherman’s syndrome that cannot be treated with surgery or hormonal therapy. Umbilical cord-derived mesenchymal stem cells (UCMSCs) have been reported to repair the damaged endometrium. However, there is no report on the effects of UCMSCs previously seeded on human acellular amniotic matrix (AAM) on endometrial injury. Methods. Absolute ethanol was injected into rat uteri to damage the endometrium. UCMSCs previously seeded on AAM were surgically transplanted. Using a variety of methods, the treatment response was assessed by endometrial thickness, endometrial biomarker expression, endometrial receptivity, cell proliferation, and inflammatory factors. Results. Endometrial thickness was markedly improved after UCMSC-AAM transplantation. The expression of endometrial biomarkers, namely, vimentin, cytokeratin, and integrin β3, in treated rats increased compared with untreated rats. In the UCMSC-AAM group, the VEGF expression decreased, whereas that of MMP9 increased compared with the injury group. Moreover, in the AAM group, the MMP9 expression increased. The expression of proinflammatory factors (IL-2, TNFα, and IFN-γ) in the UCMSC-AAM group decreased compared with the untreated group, whereas the expression of anti-inflammatory factors (IL-4, IL-10) increased significantly. Conclusions. UCMSC transplantation using AAM as the carrier can be applied to treat endometrial injury in rats. The successful preparation of lyophilized AAM provides the possibility of secondary infectious disease screening and amniotic matrix quality detection, followed by retrospective analysis. The UCMSC-AAM complex may promote the better application of UCMSCs on the treatment of injured endometrium.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Human Acellular Amniotic Matrix with Previously Seeded Umbilical Cord Mesenchymal Stem Cells Restores Endometrial Function in a Rat Model of Injury

Wang

Shi

Cai

et al. 2021

Mediators of Inflammation

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“…Specifically, STRO-1+cKit+FLK-1+CD34+ cells were reported to have the highest growth potential and neurogenic commitment, while STRO-1+cK-it+CD34+ alone express significantly more low affin- [11] general: STRO-1, CD29, CD44, CD73, CD90, CD105, CD146, CD166, CD271 hematopoietic: CD34 and CD117 stemness: OCT-3/4 and NANOG [25] CD13, CD29, CD105, CD146, CD166 [1] CD90, CD73, CD34 [49] STRO-1, c-Kit, CD29, CD34, CD44, CD73, CD105, CD146 [47] STRO-1, c-Kit, CD34 [15] CD90, CD73, CD34 ity nerve growth factor receptor (LNGFR) [47,48]. Therefore, mainly STRO-1+ DPSCs show generally firmer tendency for neurogenic commitment rather than other pulp cells expressing common mesenchymal markers [49,50]. Efficiency of novel DPSC derived treatments is strongly dependent on other cofactors, with root canal treatment recently improved through establishment of silicate -based root canal sealers that exhibited better mineralization activity compared with conventional resin -based sealers [43].…”

Section: Research Perspectivesmentioning

confidence: 99%

Human Dental Pulp Stem Cells: recent findings and current research

Janowicz

Mozdziak

Bryja

et al. 2019

Medical Journal of Cell Biology

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Prevalence of neurodegenerative diseases, most of which are life threatening and incurable, is an increasing clinical problem. To date, studies have demonstrated a superior proliferation rate of dental pulp stem cells (DPSCs) compared to other mesenchymal stem cells in vitro. DPSCs has recently been recognized as a novel treatment strategy for neurodegenerative disease, due to their advanced potential for neurogenic differentiation. The oral cavity has been described as a promising source of dental pulp stem cells. DPSCs are widely used in regenerative dentistry holding alternative capacity for osteogenic differentiation and therefore new promises for tissue and whole tooth regeneration. Dental stem cell banking offers a plentiful source of stem cells representing great potential for cell reprogramming and thus cell therapy. Recently, the association of pulp stem cells with three – dimensional scaffold templates allows for building up naturally derived implants. This review introduces to unique properties of DPSCs and biological factors influencing mineralization, proliferation and differentiation of pulp stem cells. Latest research studies are compared in terms of effectiveness and limitations of techniques for the isolation of pulp stem cells, including the enzymatic digestion and the explant culture methods. Moreover, a short overview of most recent findings and clinical application of DPSCs is proffered including progress of current research and limitations still to be addressed in the nearest future. Finally, the article presents new advances in the area of regenerative dentistry and regenerative medicine, including three dimensional printing and three dimensional analysis, emerged to deepen studies under procedures to replace the non patient specific artificial implants.Running title: DPSCs - review

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“…At present, many treatments, including pharmacotherapy, hyperbaric oxygen therapy, and neuromodulation techniques (deep brain electrical stimulation, 5,6 spinal cord electrical stimulation, 7,8 and cortical nerve stimulation 9 ), are used to treat DOC following TBI, but their efficacy is highly variable. With the development of stem cell technology, stem cells have taken on an important therapeutic role as "seed cells 10 " or "carrier cells 11,12 " in clinical practice. In fact, stem cell therapy has the potential as a treatment for DOC 13 .…”

Section: Introductionmentioning

confidence: 99%

Transplantation of glutamatergic neuronal precursor cells in the paraventricular thalamus and claustrum facilitates awakening with recovery of consciousness

Zhao

Wei

et al. 2023

CNS Neurosci Ther

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Background: Stem cells offer a promising therapeutic strategy for patients with disorders of consciousness (DOC) after severe traumatic brain injury (TBI), but the optimal transplantation sites and cells are not clear. Although the paraventricular thalamus (PVT) and claustrum (CLA) are associated with consciousness and are candidate transplantation targets, few studies have been designed to investigate this possibility. Methods: Controlled cortical injury (CCI) was performed to establish a mouse model of DOC. CCI-DOC paradigm was established to investigate the role of excitatory neurons of PVT and CLA in disorders of consciousness. The role of excitatory neuron transplantation in promoting arousal and recovery of consciousness was determined by optogenetics, chemogenetics, electrophysiology, Western blot, RT-PCR, double immunofluorescence labeling, and neurobehavioral experiments.Results: After CCI-DOC, neuronal apoptosis was found to be concentrated in the PVT and CLA. Prolonged awaking latency and cognitive decline were also seen after destruction of the PVT and CLA, suggesting that the PVT and CLA may be key nuclei in DOC. Awaking latency and cognitive performance could be altered by inhibiting or activating excitatory neurons, implying that excitatory neurons may play an important role in DOC. Furthermore, we found that the PVT and CLA function differently, with the PVT mainly involved in arousal maintenance while the CLA plays a role mainly in the generation of conscious content. Finally, we found that by transplanting excitatory neuron precursor cells in the PVT and CLA, respectively, we could facilitate

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A Reliable Stem Cell Carrier: An Experimental Study in Wistar Rats

Cited by 4 publications

References 31 publications

Human Acellular Amniotic Matrix with Previously Seeded Umbilical Cord Mesenchymal Stem Cells Restores Endometrial Function in a Rat Model of Injury

Human Acellular Amniotic Matrix with Previously Seeded Umbilical Cord Mesenchymal Stem Cells Restores Endometrial Function in a Rat Model of Injury

Human Dental Pulp Stem Cells: recent findings and current research

Transplantation of glutamatergic neuronal precursor cells in the paraventricular thalamus and claustrum facilitates awakening with recovery of consciousness

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