A Reliable Preclinical Model to Study the Impact of Cigarette Smoke in Development and Disease

Abstract

The World Health Organization has estimated that, worldwide, cigarette smoking has caused more than 100 million deaths in the last century, a number that is expected to increase in the future. Understanding cigarette smoke toxicity is key for research and development of proper public health policies. The current challenge is to establish a reliable preclinical model to evaluate the effects of cigarette smoke. In this work, we describe a simple method that allows for quantifying the toxic effects of cigarette smoke using zebrafish. Here, viability of larvae and adult fish, as well as the effects of cigarette smoke extracts on vascular development and tissue regeneration, can be easily assayed. © 2019 by John Wiley & Sons, Inc.

Modeling the effects of cigarette smoke extract on influenza B virus infections in mice

“…Cigarette smoke extract was prepared as previously described ( 55 , 56 ). Briefly, cigarette smoke from 40 commercially available Marlboro Class A Cigarettes were filtered through 12.5ml of sterile 1xPBS at a rate of 1 cigarette every 1 minutes in a chemical hood.…”

Adult and Developing Zebrafish as Suitable Models for Cardiac Electrophysiology and Pathology in Research and Industry

“…One area of research with zebrafish is the study of the adverse effects of xenobiotics on animal growth and development. For decades, zebrafish embryos/larvae have been used as biosensors in ecotoxicity studies to test the presence of contaminants and to understand how environmental endocrine disruptors, contaminants and drugs affect human development and health (Vliegenthart et al, 2014;Aluru, 2017;Aedo et al, 2019;Silveira et al, 2019). The model is useful to study the cardiac toxicity induced by pollutants from embryonic to adult stages, showing cardiac malformations and changes in heart rate and in ejection fraction (Wang et al, 2020).…”