A reliable method of liquid chromatography for the quantification of acetaminophen and identification of its toxic metabolite <i>N</i>‐acetyl‐<i>p</i>‐benzoquinoneimine for application in pediatric studies

Flores‐Pérez, Carmen; Chávez‐Pacheco, Juan Luis; Ramírez-Mendiola, Blanca; Alemón-Medina, Radamés; García-Álvarez, Raquel; Juárez-Olguı́n, Hugo; Flores‐Pérez, Janett

doi:10.1002/bmc.1511

Cited by 21 publications

(7 citation statements)

References 20 publications

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“…11) The concentrations of MDZ were determined by HPLC including an UV detector (SPD-20Av, Shimadzu) following an established method. 12) The APAP, and APAP-glucuronide concentrations were determined by HPLC via an UV detector as previously reported 13) with minor modifications. The sample was mixed with methanol and 1 mg/mL salicylamide (internal standard), and then centrifuged at 17860× g for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Effect of Chronic Kidney Disease on Hepatic Clearance of Drugs in Rats

Tokunaga

Miyamoto

Fumoto

et al. 2020

Biological & Pharmaceutical Bulletin

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The pharmacokinetics of some hepatically cleared drugs have been reported to fluctuate in patients with renal impairment, but the definitive factors have not been clarified. We compared the pharmacokinetics of some drugs with different hepatic elimination processes in a chronic kidney disease (CKD) rat model, to optimize their administration during kidney injury. We chose indocyanine green (ICG), midazolam (MDZ), and acetaminophen (APAP) as reference drugs to determine changes in hepatic clearance pathways in presence of CKD. Drugs were intravenously administered via the jugular vein to the CKD model rats, previously established by adenine administration, and then, blood, bile, and urine samples were collected. The plasma concentration of ICG, which is eliminated into the bile without biotransformation, increased; and its total body clearance (CL tot) significantly decreased in the CKD group compared to the control group. Moreover, the plasma concentrations of MDZ and APAP, metabolized in the liver by CYP3A and Ugt1a6 enzymes, respectively, were higher in the CKD group than in the control group. The biliary clearances of APAP and its derivative APAP-glucuronide increased in the CKD group, whereas their renal clearances were markedly decreased with respect to those in the control group. Altogether, plasma concentrations of some hepatically eliminated drugs increased in the CKD rat model, but depending on their pharmacokinetic characteristics. This study provides useful information for optimizing the administration of some hepatically cleared drugs in CKD patients.

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Section: Methodsmentioning

confidence: 99%

Effect of Chronic Kidney Disease on Hepatic Clearance of Drugs in Rats

Tokunaga

Miyamoto

Fumoto

et al. 2020

Biological & Pharmaceutical Bulletin

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“…After fasting for 24 h, the dogs were given 20 g bread and 200 ml milk containing acetaminophen at a dose of 0.025 g/kg body weight. After 45 min, blood samples were collected, and the acetaminophen concentration in blood was determined using high performance liquid chromatography (HPLC) [12].…”

Section: Methodsmentioning

confidence: 99%

Reconstruction of the Abdominal Vagus Nerve Using Sural Nerve Grafts in Canine Models

et al. 2013

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BackgroundRecently, vagus nerve preservation or reconstruction of vagus has received increasing attention. The present study aimed to investigate the feasibility of reconstructing the severed vagal trunk using an autologous sural nerve graft.MethodsTen adult Beagle dogs were randomly assigned to two groups of five, the nerve grafting group (TG) and the vagal resection group (VG). The gastric secretion and emptying functions in both groups were assessed using Hollander insulin and acetaminophen tests before surgery and three months after surgery. All dogs underwent laparotomy under general anesthesia. In TG group, latency and conduction velocity of the action potential in a vagal trunk were measured, and then nerves of 4 cm long were cut from the abdominal anterior and posterior vagal trunks. Two segments of autologous sural nerve were collected for performing end-to-end anastomoses with the cut ends of vagal trunk (8–0 nylon suture, 3 sutures for each anastomosis). Dogs in VG group only underwent partial resections of the anterior and posterior vagal trunks. Laparotomy was performed in dogs of TG group, and latency and conduction velocity of the action potential in their vagal trunks were measured. The grafted nerve segment was removed, and stained with anti-neurofilament protein and toluidine blue.ResultsLatency of the action potential in the vagal trunk was longer after surgery than before surgery in TG group, while the conduction velocity was lower after surgery. The gastric secretion and emptying functions were weaker after surgery in dogs of both groups, but in TG group they were significantly better than in VG group. Anti-neurofilament protein staining and toluidine blue staining showed there were nerve fibers crossing the anastomosis of the vagus and sural nerves in dogs of TG group.ConclusionReconstruction of the vagus nerve using the sural nerve is technically feasible.

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“…There are a number of published reports describing the determination of acetaminophen or its metabolites in biological samples based on high‐performance liquid chromatography with ultraviolet detection (HPLC‐UV) (Flores‐Perez et al, ; Goicoechea, De Alda, & Vila‐Jato, ; Helmy & El‐Bedaiwy, ; Ibanez et al, ; Jensen, Valentine, Milne, & Evans, ; Yin, Tomlinson, Chow, & Chow, ), liquid chromatography mass spectrometry (LC‐MS) (Teffera & Abramson, ) and liquid chromatography–tandem mass spectrometry (LC‐MS/MS) (Cook, King, Van der Anker, & Wilkins, ; Hewavitharana, Lee, Dawson, Markovich, & Shaw, ; Im et al, ; Liao et al, ; Swales, Temesi, Denn, & Murphy, ; Tan et al, ; Yin, Lam, & Chow, ; Zou et al, ). However, none of these studies demonstrate the quantification of acetaminophen or its metabolites in liver and kidney since these two organs are the major victims of acetaminophen overdose (Adil et al, ; Bunchorntavakul & Reddy, ; Inoue et al, ; James et al, ; Stollings et al, ).…”

Section: Introductionmentioning

confidence: 99%

A reliable LC‐MS/MS method for the quantification of N‐acetyl‐p‐benzoquinoneimine, acetaminophen glutathione and acetaminophen glucuronide in mouse plasma, liver and kidney: Method validation and application to a pharmacokinetic study

Zhang

et al. 2018

Biomedical Chromatography

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A rapid, specific, and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated to simultaneously quantify N-acetyl-p-benzoquinoneimine (NAPQI), acetaminophen-glutathione (acetaminophen-glut) and acetaminophen-glucuronide (acetaminophen-gluc) in mouse plasma, liver and kidney homogenates. Analytes were eluted by a binary gradient mobile phase composed of water (phase A) and methanol containing 0.1% formic acid (phase B) at a flow rate of 0.3 mL/min, which was performed on a CAPCELL PAK C MG II column. It took 3.2 min to detect three analytes in a single run. Quantification was carried out in positive mode combined with multiple reaction monitoring. The validation of the LC-MS/MS method consisted of specificity, linearity, precision, accuracy, protein precipitation recovery, matrix effect, dilution integrity and stability. The plasma and tissue homogenate calibration curves were linear over concentration ranges of 0.050-5.00, 0.050-5.00 and 0.100-40.0 μg/mL, with a lower limit of quantification of 0.050, 0.050, and 0.100 μg/mL for NAPQI, acetaminophen-glut and acetaminophen-gluc, respectively. The intra- and inter-run precision values were within 12.47% for NAPQI, 12.11% for acetaminophen-glut and 11.86% for acetaminophen-gluc at their lower limit of quantitation levels. The samples were stable under all tested conditions. This method was successfully applied to study the pharmacokinetics of NAPQI, acetaminophen-glut and acetaminophen-gluc in ICR mice following oral administration of 200 mg/kg of acetaminophen suspension.

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A reliable method of liquid chromatography for the quantification of acetaminophen and identification of its toxic metabolite N‐acetyl‐p‐benzoquinoneimine for application in pediatric studies

Cited by 21 publications

References 20 publications

Effect of Chronic Kidney Disease on Hepatic Clearance of Drugs in Rats

Effect of Chronic Kidney Disease on Hepatic Clearance of Drugs in Rats

Reconstruction of the Abdominal Vagus Nerve Using Sural Nerve Grafts in Canine Models

A reliable LC‐MS/MS method for the quantification of N‐acetyl‐p‐benzoquinoneimine, acetaminophen glutathione and acetaminophen glucuronide in mouse plasma, liver and kidney: Method validation and application to a pharmacokinetic study

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