A reliable liquid chromatography–tandem mass spectrometry method for simultaneous determination of multiple mycotoxins in fresh fish and dried seafoods

Sun, Wenshuo; Han, Zheng; Aerts, Johan; Nie, Dongxia; Jin, Mengtong; Shi, Wentao; Zhao, Zhiyong; Saeger, Sarah De; Yong, Zhao; Wu, Aibo

doi:10.1016/j.chroma.2015.01.071

Cited by 52 publications

(39 citation statements)

References 32 publications

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“…The results showed that within the wide‐testing concentration range of each analyte, a good linear relationship existed, with coefficient of determination ( R 2 ) between 0.9992 (AFG 1 and OTA) and 0.9997 (AFG 2 ). In general, nice and satisfactory sensitivities were obtained with LODs varying from 0.031 to 2.5 μg/kg and LOQs in the range of 0.078–6.25 μg/kg for all the seven analytes, which are obviously better than the sensitivities of the previously established methods for determination of multiple mycotoxins in Lentinula edodes , fish, seafood, and egg samples .…”

Section: Resultsmentioning

confidence: 73%

Development of an economic ultrafast liquid chromatography with tandem mass spectrometry method for trace analysis of multiclass mycotoxins in Polygonum multiflorum

Huang

Liu

Wang

et al. 2018

J of Separation Science

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Rapid, economic, and highly effective determination of multiple mycotoxins in complex matrices has given huge challenges for the analytical method. In this study, an economic analytical strategy based on sensitive and rapid ultrafast liquid chromatography coupled to hybrid triple quadrupole/linear ion trap mass spectrometry technique was developed for the determination of seven mycotoxins of different chemical classes (aflatoxin B1, B2, G1, and G2, ochratoxin A, T‐2 toxin, and HT‐2 toxin) in Polygonum multiflorum. Target mycotoxins were completely extracted using a modified quick, easy, cheap effective, rugged, and safe method without additional clean‐up steps. The types of extraction solvents and adsorbents for the extraction procedure were optimized to achieve high recoveries and reduce coextractives in the final extracts. Due to significant matrix effects for all analytes (≤68.9% and ≥110.0%), matrix‐matched calibration curves were introduced for reliable quantification, exploring excellent linearity for the seven mycotoxins with coefficients of determination >0.9992. The method allowed high sensitivity with limit of detection in the range of 0.031–2.5 μg/kg and limit of quantitation in the range of 0.078–6.25 μg/kg, as well as satisfactory precision with relative standard deviations lower than 8%. Recovery rates were between 74.3 and 119.8% with relative standard deviations below 7.43%. The proposed method was successfully applied for 24 batches of P. multiflorum samples, and six samples were found to be positive with aflatoxin B1, B2, G1, or ochratoxin A. The method with significant advantages, including minimum analytical time, low time and solvent consumption, and high sensitivity, would be a preferred candidate for economic analysis of multiclass mycotoxins in complex matrices.

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Section: Resultsmentioning

confidence: 73%

Development of an economic ultrafast liquid chromatography with tandem mass spectrometry method for trace analysis of multiclass mycotoxins in Polygonum multiflorum

Huang

Liu

Wang

et al. 2018

J of Separation Science

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show abstract

“…In previous literature [39,40], often an additional matrix-matched calibration experiment was required to evaluate matrix effect. However, due to the extremely complex and varying interferences in different cereals, this method is not appropriate any more.…”

Section: Matrix Effectmentioning

confidence: 99%

“…Analytical methods for determination of mycotoxins have been reviewed [10,11], among them liquid chromatographic (LC) coupled with various detectors (e.g. fluorescent or tandem mass spectrometry detector) are the most widely used methods at present [12][13][14][15][16], in particular, liquid chromatography with tandem mass spectrometry (LC-MS 2 ) is recognized as the "gold standard" approach for mycotoxin analysis due to the natural advantages of its high sensitivity and selectivity.…”

Section: Introductionmentioning

confidence: 99%

Chemometrics-enhanced liquid chromatography-full scan-mass spectrometry for interference-free analysis of multi-class mycotoxins in complex cereal samples

Liu

Xie

et al. 2017

Chemometrics and Intelligent Laboratory Systems

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“…Instrumental methods produce sensitive and reliable results but they are laborious, costly and conducted in a sequential manner, making them unsuitable for routine analysis of large scale of samples [19,20].…”

Section: Introductionmentioning

confidence: 99%

Anti-idiotypic VHH phage display-mediated immuno-PCR for ultrasensitive determination of mycotoxin zearalenone in cereals

Wang

et al. 2016

Talanta

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A reliable liquid chromatography–tandem mass spectrometry method for simultaneous determination of multiple mycotoxins in fresh fish and dried seafoods

Cited by 52 publications

References 32 publications

Development of an economic ultrafast liquid chromatography with tandem mass spectrometry method for trace analysis of multiclass mycotoxins in Polygonum multiflorum

Development of an economic ultrafast liquid chromatography with tandem mass spectrometry method for trace analysis of multiclass mycotoxins in Polygonum multiflorum

Chemometrics-enhanced liquid chromatography-full scan-mass spectrometry for interference-free analysis of multi-class mycotoxins in complex cereal samples

Anti-idiotypic VHH phage display-mediated immuno-PCR for ultrasensitive determination of mycotoxin zearalenone in cereals

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