Ultra-performance liquid chromatography (UPLC) was used to detect six fluoroquinolones (FQs) in live aquatic produce sampled from the region surrounding the Dongting Lake in Hunan, China. Results showed that 8.3% of samples were contaminated with norfloxacin in the range of 4.5 to 10.3 µg/kg, and 7.3% of samples carried residual ofloxacin in the range of 6.7 to 76.0 µg/kg. One crucian carp sample was pefloxacin-positive, and one blunt snout bream sample was lomefloxacin-positive, and the level in these two samples were 18.3 and 25.1 µg/kg, respectively. Further, 3.1% of samples contained residues of enrofloxacin and (or) ciprofloxacin higher than their maximum residue limits. This survey demonstrated that banned drugs were occasionally used, and that enrofloxacin was occasionally used in excess in aquaculture in the surveyed area.