A reliable and sensitive method for fluorescent photoconversion

Singleton, Chasidy; Casagrande, Vivien A.

doi:10.1016/0165-0270(95)00097-6

Cited by 34 publications

(27 citation statements)

References 13 publications

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“…After culture, embryos were placed in phosphate buffered saline (PBS) and observed with an inverted microscope (Leica) under light or Ultra Violet and pictured. In few cases, the DiI signal was photoconverted to diaminobenzidine staining (Singleton and Casagrande, 1996).…”

Section: Cell Lineage Analysismentioning

confidence: 99%

The DVE changes distal epiblast fate from definitive endoderm to neurectoderm by antagonizing nodal signaling

Miura¹,

Mishina²

2007

Developmental Dynamics

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To assess the function of the distal visceral endoderm (DVE) of embryonic day 5.5 (E5.5) embryos, we established a system to directly ablate the DVE and observe the consequences after culture. When the DVE was successfully ablated, such embryos (DVE-ablated embryos) showed deregulated expression of Nodal and Wnt3 and ectopically formed the primitive streak at the proximal portion of the embryo. The DVE and anterior visceral endoderm (AVE) are implicated in the development of neurectoderm. We found that the distal epiblast of E5.

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Section: Cell Lineage Analysismentioning

confidence: 99%

The DVE changes distal epiblast fate from definitive endoderm to neurectoderm by antagonizing nodal signaling

Miura¹,

Mishina²

2007

Developmental Dynamics

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“…To aid in analysis, some sections were also counterstained with Giemsa to reveal the cell layers in the LGN. Giemsa counterstaining was carried out according to the protocol of Singleton and Casagrande (1996).…”

Section: Histological Proceduresmentioning

confidence: 99%

Organization of the feedback pathway from striate cortex (V1) to the lateral geniculate nucleus (LGN) in the owl monkey (Aotus trivirgatus)

Ichida

Casagrande

2002

J of Comparative Neurology

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The function of the corticogeniculate feedback pathway from the striate cortex (V1) to the lateral geniculate nucleus (LGN) in primates is not well understood. Insight into possible function can be gained by studying the morphology and projection patterns of corticogeniculate axons in the LGN. The goal of this research was to examine how corticogeniculate axons innervate the functionally specific (e.g., parvocellular [P], magnocellular [M], and koniocellular [K]) and eye-specific layers of the LGN. Pressure injections of biotinylated dextran were made into owl monkey V1, and the resulting labeled axons were reconstructed through serial sections of the LGN. All of the corticogeniculate axons, regardless of termination pattern, were thin with boutons en passant or at the ends of small stalks, as described in cats. Axons were found in all layers of the LGN, and two main patterns of innervation were observed. In the first pattern, axons terminated in individual M or P LGN layers. In the second pattern of innervation, axons terminated in pairs of functionally matched layers. Examples of this type were seen within pairs of M, P, or K layers. In most cases, both classes of axons contain arbors focused within the P or M layers but also had collateral side branches in neighboring K layers. Unlike corticogeniculate axons seen in the cat, corticogeniculate axons in the owl monkey maintained topographic innervation in the LGN layers that was consistent with receptive field sizes represented in V1. The patterns of layer projections along with the retinotopic match of corticogeniculate axons within the LGN suggest that in primates V1 can modulate activity in the LGN through functionally specific projections in a more tightly tuned retinotopic fashion than previously believed.

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“…Photoconversion was carried out essentially as previously described using 1.5 mg/ml diaminobenzidine (Kirkegaard & Perry) in Tris buffer (pH 8.2) [5] , however, we carried out our photoconversion on intact ganglia (prior to cryosectioning). DAB precipitation did not occur in unlabeled ganglia.…”

Section: Neurotrophic Factor Receptor Mrna Expression Analysismentioning

confidence: 99%

Neurotrophic Factor Receptor Expression and in vitro Nerve Growth of Geniculate Ganglion Neurons That Supply Divergent Nerves

Yamout

Spec²,

Cosmano³

et al. 2005

Dev Neurosci

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We investigated which neurotrophic factors may contribute to the divergence of two peripheral nerves emanating from the geniculate ganglion. We compared receptor mRNA profiles of the neurons that supply the nerves, and also the growth of their neurites in response to neurotrophic factors in culture. Three mRNAs, Gfra2, TrkA, and TrkC, were differentially expressed. Only one ligand, Neurturin, promoted substantially different nerve regrowth from the nerves, and therefore may contribute to nerve divergence. Three receptor mRNAs were expressed in 100% of the neurons: TrkB, TrkB.T2 (kinase-lacking isoform), and NCAM-140. Ligands for these Trks and FRα-1 promoted more outgrowth than ligands for the other receptors. NT-3 and BDNF synergistically promoted outgrowth. Finally, receptors are coexpressed at random rates, arguing against the existence of neuronal subtypes defined by a combinatorial code of these receptors.

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A reliable and sensitive method for fluorescent photoconversion

Cited by 34 publications

References 13 publications

The DVE changes distal epiblast fate from definitive endoderm to neurectoderm by antagonizing nodal signaling

The DVE changes distal epiblast fate from definitive endoderm to neurectoderm by antagonizing nodal signaling

Organization of the feedback pathway from striate cortex (V1) to the lateral geniculate nucleus (LGN) in the owl monkey (Aotus trivirgatus)

Neurotrophic Factor Receptor Expression and in vitro Nerve Growth of Geniculate Ganglion Neurons That Supply Divergent Nerves

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