A reliable and accurate UHPLC-MS/MS method for screening of Aspergillus, Penicillium and Alternaria mycotoxins in orange, grape and apple juices

Guo, Wenbo; Yang, Junhua; Niu, Xue-Ke; Tangni, Emmanuel K.; Zhao, Zhihui; Han, Zheng

doi:10.1039/d0ay01787f

Cited by 19 publications

(8 citation statements)

References 28 publications

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“…3, we can visualize the detection rate and concentration range of different kinds of toxins, among which the detection rate and residual amount of TeA are relatively high compared with the other four kinds of toxins. This is consistent with previous reports 15,16,18,20,22,44‐48 . Previously reported data on Alternaria toxins content in fruits and products are presented in Table 8.…”

Section: Resultssupporting

confidence: 93%

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Saturated brine dissolution and liquid–liquid extraction combined with UPLC–MS/MS for the detection of typical Alternaria toxins in pear paste

Jiang

Zang

et al. 2023

J Sci Food Agric

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BACKGROUNDAlternaria can infest pears to produce metabolites, which can contaminate pears and their processed products. Pear paste, one of the most important pear‐based products, is popular among Chinese consumers especially for its cough relieving and phlegm removal properties. Although people are concerned about the risk of Alternaria toxins in many agro‐foods and their products, little is known about the toxins in pear paste.RESULTSA method was developed for the determination of tenuazonic acid, alternariol, alternariol menomethyl ether, altenuene and tentoxin in pear paste by ultra‐performance liquid chromatography tandem mass spectrometry with saturated sodium sulphate dissolution and acidified acetonitrile extraction. The mean recoveries of the five toxins were 75.3–113.8% with relative standard deviations of 2.8–12.2% at spiked levels of 1.0–100 μg kg−1. Alternaria toxins were detected in 53 out of 76 samples, with a detection rate of 71.4%. Tenuazonic acid (67.1%), alternariol (35.5%), tentoxin (23.7%) and alternariol monomethyl ether (7.9%) were detected in all samples at concentrations of < limit of quantification (LOQ)–105.0 μg kg−1, < LOQ–32.1 μg kg−1, < LOQ–74.2 μg kg−1 and < LOQ–15.1 μg kg−1, respectively. Altenuene was never found in pear paste samples. Tenuazonic acid, alternariol, tentoxin and alternariol menomethyl ether should be focused on due to their toxicity and detection rates.CONCLUSIONTo the best of our knowledge, this is the first report on the detection method and residue levels of Alternaria toxins in pear paste. The proposed method and research data can provide technical support for the Chinese government to continuously monitor and control Alternaria toxins in pear paste, especially tenuazonic acid. It can also provide a useful reference for related researchers. © 2023 Society of Chemical Industry.

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Section: Resultssupporting

confidence: 93%

“…This is consistent with previous reports. 15,16,18,20,22,[44][45][46][47][48] Previously reported data on Alternaria toxins content in fruits and products are presented in Table 8. TeA and TEN are commonly found in fresh fruits and vegetables.…”

Section: Analysis Of Pear Paste Samplesmentioning

confidence: 99%

Saturated brine dissolution and liquid–liquid extraction combined with UPLC–MS/MS for the detection of typical Alternaria toxins in pear paste

Jiang

Zang

et al. 2023

J Sci Food Agric

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“…Among Alternaria toxins, AOH is the most predominantly occurring in food and is related with mutagenicity and genotoxicity [ 25 ]. Both AFB1 and AOH have been reported by several authors in different types of juices [ 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 96%

High Pressure Processing Impact on Alternariol and Aflatoxins of Grape Juice and Fruit Juice-Milk Based Beverages

et al. 2021

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High-pressure processing (HPP) has emerged over the last 2 decades as a good alternative to traditional thermal treatment for food safety and shelf-life extension, supplying foods with similar characteristics to those of fresh products. Currently, HPP has also been proposed as a useful tool to reduce food contaminants, such as pesticides and mycotoxins. The aim of the present study is to explore the effect of HPP technology at 600 MPa during 5 min at room temperature on alternariol (AOH) and aflatoxin B1 (AFB1) mycotoxins reduction in different juice models. The effect of HPP has also been compared with a thermal treatment performed at 90 °C during 21 s. For this, different juice models, orange juice/milk beverage, strawberry juice/milk beverage and grape juice, were prepared and spiked individually with AOH and AFB1 at a concentration of 100 µg/L. After HPP and thermal treatments, mycotoxins were extracted from treated samples and controls by dispersive liquid–liquid microextraction (DLLME) and determined by HPLC-MS/MS-IT. The results obtained revealed reduction percentages up to 24% for AFB1 and 37% for AOH. Comparing between different juice models, significant differences were observed for AFB1 residues in orange juice/milk versus strawberry juice/milk beverages after HPP treatment. Moreover, HPP resulted as more effective than thermal treatment, being an effective tool to incorporate to food industry in order to reach mycotoxins reductions.

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“…Fruit juice and purées are a food category of interest for the development of multi-analyte methods, in consideration of the reported increasing fungal infections due to climate change. Guo et al (2021) presented a LC-MS/MS protocol for the quantification of 15 major mycotoxins produced by Aspergillus, Penicillium and Alternaria species in orange, grape, and apple juices. The extraction procedure was based on the use of QuEChERS, and allowed for satisfactory recoveries (74-110%) and sensitivity (LODs: 0.05-0.1 µg/l).…”

Section: Chromatography With Ms/msmentioning

confidence: 99%

Developments in mycotoxin analysis: an update for 2020-2021

Tittlemier¹,

Cramer

Dall’Asta

et al. 2022

WMJ

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This review summarises developments published in the period from mid-2020 to mid-2021 on the analysis of a number of diverse matrices for mycotoxins. Notable developments in all aspects of mycotoxin analysis, from sampling and quality assurance/quality control of analytical results, to the various detection and quantitation technologies ranging from single mycotoxin biosensors to comprehensive instrumental methods are presented and discussed. The summary and discussion of this past year’s developments in detection and quantitation technology covers chromatography with targeted or non-targeted high resolution mass spectrometry, tandem mass spectrometry, detection other than mass spectrometry, biosensors, as well as assays using alternatives to antibodies. This critical review aims to briefly present the most important recent developments and trends in mycotoxin determination, as well as to address limitations of the presented methodologies.

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A reliable and accurate UHPLC-MS/MS method for screening of Aspergillus, Penicillium and Alternaria mycotoxins in orange, grape and apple juices

Abstract: An ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) method was developed for simultaneous determination of 15 mycotoxins, including aflatoxins (B1, B2, G1, G2), ochratoxins (A, B, C), citrinin, patulin, and...

Cited by 19 publications

References 28 publications

Saturated brine dissolution and liquid–liquid extraction combined with UPLC–MS/MS for the detection of typical Alternaria toxins in pear paste

Saturated brine dissolution and liquid–liquid extraction combined with UPLC–MS/MS for the detection of typical Alternaria toxins in pear paste

High Pressure Processing Impact on Alternariol and Aflatoxins of Grape Juice and Fruit Juice-Milk Based Beverages

Developments in mycotoxin analysis: an update for 2020-2021

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