A refined technique for sciatic denervation in a golden-mantled ground squirrel (Callospermophilus lateralis) model of disuse atrophy

Abstract

Disuse atrophy of both muscle and bone can occur rapidly during periods of inactivity. In several rodent models developed for the study of disuse atrophy, immobilization is induced by prolonged cage restraint, hind limb unloading, tenotomy, sciatic nerve block or sciatic denervation. In less tractable species such as wild-caught hibernating rodents, the sciatic denervation model is superior in terms of both animal welfare and applicability to the characteristics of natural cases of disuse atrophy. The authors describe a refined surgical approach to sciatic denervation in golden-mantled ground squirrels (Callospermophilus lateralis), a hibernating species, that improves animal welfare and reduces the incidence of post-operative complications such as autotomy.

Maslinic Acid Attenuates Denervation-Induced Loss of Skeletal Muscle Mass and Strength

“…The sciatic-nerve transection model is commonly employed to induce denervation [ 24 ]. In the present study, we confirmed that dietary intake of MA effectively prevented the loss of muscle weight and strength on day 14 after sciatic nerve denervation ( Figure 1 C–E).…”