A refined sampling strategy for intra-tooth stable isotope analysis of mammalian enamel

Zazzo, Antoine; Bendrey, Robin; Vella, Dominic; Moloney, A.P.; Monahan, Frank J.; Schmidt, Olaf

doi:10.1016/j.gca.2012.01.012

Cited by 73 publications

(50 citation statements)

References 49 publications

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“…Tooth enamel is preferentially sampled for sequential analysis of bioapatite, as attempted for the first time by Fricke and O'Neil (1996). In spite of methodological issues due to the complexity of the process of enamel deposition and mineralization (Balasse, 2002(Balasse, , 2003Balasse et al, 2011;Fricke et al, 1998a;Blaise and Balasse, 2011;Hoppe et al, 2004;Cerling, 2002, 2004;Passey et al, 2005a;Zazzo et al, 2005Zazzo et al, , 2010Zazzo et al, , 2012, it has since been widely applied to modern and archaeological specimens including low and high crowned teeth, as well as evergrowing teeth, to investigate paleoecology and paleoclimatology (Bernard et al, 2009;Dam and Reichart, 2009;Botha et al, 2005;Fox and Fisher, 2001;Fricke et al, 1998b;Fraser et al, 2008;Nelson, 2005;Sharp and Cerling, 1998;Stuart-Williams et al, 1997;Zazzo et al, 2002), seasonal mobility of wild and domestic herds and associated human mobility Balasse et al, 2002;Bentley and Knipper, 2005;Britton et al, 2009Britton et al, , 2011Copeland et al, 2008;Henton et al, 2010;Pellegrini et al, 2008), diet (Balasse et al, , 2006(Balasse et al, , 2009Copeland et al, 2009;Cerling et al, 2008;Feranec et al, 2009) and birth seasonality (Balasse and Tresset, 2007;Balasse et al, 2003;…”

Section: Introductionmentioning

confidence: 99%

Seasonality of birth and diet of pigs from stable isotope analyses of tooth enamel (δ18O, δ13C): a modern reference data set from Corsica, France

Frémondeau

Cucchi

Casabianca

et al. 2012

Journal of Archaeological Science

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a b s t r a c tSince domestication during the mid 11th millennium BP in the Near East and the 8th millennium BP in China, the pig has played an important role in human diet and economy. The objective of this study is to evaluate the potential of stable oxygen and carbon isotopes sequential analyses of pig tooth enamel to investigate pig seasonality of birth and diet, as these are important parameters for the reconstruction of pig herding strategies in ancient societies.A pilot study was performed on a modern reference set composed of five free-range Corsican domestic pigs and four Corsican feral pigs. Tooth enamel from the mandibular incisors (I1, I2), molars (M2, M3) and canines (C) were sampled sequentially to map the stable oxygen and carbon isotope records in the pig mandible.The sequences of d 18 O published in this paper may be used as a reference data set for births grouped in mid-autumn and late winter. The d 13 C values measured in the wild pigs constitute the first reference set for a wild population living in a Mediterranean environment. Results show that among all sampled teeth (I1, I2, C, M1 and M2), the combination of the sequences of d 18 O measured in the first and second incisors provides the most effective data for determination of birth seasonality. The sequences of d 18 O measured in the second and third molars were too short and/or dampened to be interpreted in terms of seasonality. In domestic pigs, male evergrowing canines provide a one and a half year record, allowing observation of short-term seasonal variations in the isotopic composition of diet.Applied to archaeological assemblages, the joint stable isotope analysis of incisors and canines will enable the qualification of the seasonal rhythm of pig herding practices, including slaughtering strategies, in more concrete terms. These practices are directly linked to the modalities of pork production but also to the availability of food resources.

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Section: Introductionmentioning

confidence: 99%

Seasonality of birth and diet of pigs from stable isotope analyses of tooth enamel (δ18O, δ13C): a modern reference data set from Corsica, France

Frémondeau

Cucchi

Casabianca

et al. 2012

Journal of Archaeological Science

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“…Observation of developing third molars (M 3 ) in sheep shows that the growth rate is not constant but decreases exponentially (Zazzo et al . ). Here, we consider this question for horses, which are useful for such studies as the timespan of tooth growth is longer than for sheep or cattle, and so presents a more robust test of the model.…”

Section: Introductionmentioning

confidence: 97%

“…As already stated, this model has been successfully applied to sheep teeth (Zazzo et al . ), and horse teeth provide ideal material for the extension of research into hypsodont tooth growth, as horse teeth grow over a longer time period than those of sheep. This paper draws on tooth length measurements from modern domestic horses of known age and data collected from stable isotope analyses (δ 13 C and δ 18 O) on serial enamel microsamples from archaeological horse teeth from Iron Age burial mounds at Tsenghel Khairkhan, Mongolia (Bendrey et al .…”

Section: Introductionmentioning

confidence: 99%

Exponentially decreasing tooth growth rate in horse teeth: implications for isotopic analyses

Bendrey

Vella²,

Zazzo

et al. 2014

Archaeometry

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A range of archaeological and palaeoclimatic studies use isotopic analyses of ungulate hypsodont tooth enamel. Such studies commonly assume a constant growth rate, though this has not been fully tested. Here, we use stable isotope analyses of sequential enamel samples to study horse tooth growth. We fit the data using models corresponding to constant and exponentially decreasing rates of growth, and compare the results to metrical data showing the geometry and timing of apposition. The results indicate enamel apposition and maturation advances at an exponentially decreasing rate. An understanding of this variable growth rate is crucial for interpreting isotopic data from equid teeth.

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“…Tra di tional microsampling by microdrilling of mam ma lian tooth enamel al lowed pre par ing 3 to 10 microsamples (Wiedemann-Bidlack et al, 2008;Britton et al, 2009;Tian et al, 2013;Wright, 2013, Zazzo et al, 2012, that means an av er age dis tance be tween pits in the range of about 1.2-2.0 mm. In con se quence, con ven tional micro sampling of enamel pro vides an iso to pic aver age of dif fer ent in cre men tal lay ers.…”

Section: Discussionmentioning

confidence: 99%

Reconstructing seasonality using d18O in incremental layers of human enamel: a test of the analytical protocol developed for SHRIMP IIe/MC ion microprobe

Krzemińska

Sołtysiak

Czupyt

2017

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Re con struct ing sea son al ity us ing d 18 O in in cre men tal lay ers of hu man enamel: a test of the an a lyt i cal pro to col de vel oped for SHRIMP IIe/MC ion microprobe Ewa A num ber of re cent stud ies deal ing with palaeoclimate and en vi ron men tal re con struc tion in clude the mea sure ment of ox ygen iso tope com po si tion of mam ma lian teeth. Some of them ana lyse a tem po ral se quence of the changes re corded in bioapatite from enamel lay ers rep re sent ing the whole pe riod of tooth de vel op ment. Enamel sam ples dis play large intra-tooth d 18 O vari a tions that may re flect a sea sonal fluc tu a tion in the d 18 O of lo cal palaeoclimate pa ram e ters. The pres ent pa per provides an ef fec tive an a lyt i cal pro to col for se quen tial d 18 O anal y sis of hu man teeth us ing SHRIMP IIe/MC ion microprobe. It is pos si ble to fol low the in ner enamel layer along enamel-dentine junc tion on a high spa tial scale in a range about 0.02 mm of spot di am e ter and 0.12-0.14 mm of the dis tance be tween spots. Us ing the meth od ol ogy de scribed herein, we can achieve an ex ter nal pre ci sion for d 18 O anal y sis <0.2‰ (1s). The num ber of 60 to 90 sin gle anal y ses cov er ing the enamel layer between the incisal and api cal ends is enough to ob tain tem po ral res o lu tion of less than one month and to doc u ment pre cisely sea sonal fluc tu a tion caused by lo cal en vi ron men tal and cli mate fac tors. The meth od ol ogy of d 18 O in situ mea sure ments has been tested on hu man teeth from Tell Majnuna, a 4th mil len nium BCE cem e tery in North ern Mes o po ta mia, which is a rel atively arid area with high sea sonal dif fer ences in pre cip i ta tion and tem per a ture. Ob served pat tern of d 18 O vari a tions is consis tent with ex pected sea sonal fluc tu a tions, al though the over all ef fect is blurred by some in er tia in the enamel mat u ra tion.Key words: palaeoclimate, bioapatite, hu man teeth, se quen tial microsampling, Mid dle East, Mes o po ta mia. INTRODUCTIONOx y gen iso tope anal y sis of den tal enamel is a pow er ful tool for palaeo bio logi cal and ar chae o log i cal stud ies as an in di ca tor of the pa ram e ters of phys i ol ogy, nat u ral en vi ron ment and hab itat pref er ences. A par tic u larly prom is ing ap proach is to measure intra-tooth vari a tion (Ballase, 2003;Blaise and Balasse, 2011;Stevens et al., 2011), be cause teeth ac cu mu late a sequence of sig nif i cant iso to pic in for ma tion cov er ing the short time of enamel bioapatite pro gres sive for ma tion.The ox y gen iso tope in ves ti ga tions of mam ma lian den tal enamel are based on a few gen eral as sump tions that:-the tis sues of or gan ism iso to pi cally equil i brated with their en vi ron ment will re flect the lo cal en vi ron men tal oxy gen ra tio; -iso to pic sig na ture of skel e tal tis sues is di rectly re lated to that of the lo cal drink ing wa ter; -bioapatite, the main com po nent of teeth, is rel a tively most re sis tant to diagenetic pro cesses and pre serves it...

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A refined sampling strategy for intra-tooth stable isotope analysis of mammalian enamel

Cited by 73 publications

References 49 publications

Seasonality of birth and diet of pigs from stable isotope analyses of tooth enamel (δ18O, δ13C): a modern reference data set from Corsica, France

Seasonality of birth and diet of pigs from stable isotope analyses of tooth enamel (δ18O, δ13C): a modern reference data set from Corsica, France

Exponentially decreasing tooth growth rate in horse teeth: implications for isotopic analyses

Reconstructing seasonality using d18O in incremental layers of human enamel: a test of the analytical protocol developed for SHRIMP IIe/MC ion microprobe

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