A recombinant protein (MyxoTLm) for the serological diagnosis of acute and chronic Trypanosoma vivax infection in cattle

Pinheiro, Guilherme Rafael Gomide; Ferreira, Lorena Lopes; Silva, Ana Luíza Teixeira; Cardoso, Mariana Santos; Ferreira-Júnior, A.; Steindel, Mário; Grisard, Edmundo C.; Miletti, Luiz Cláudio; Bartholomeu, Daniella Castanheira; Bueno, Lilian Lacerda; Santos, Renato Lima; Fujiwara, Ricardo Toshio

doi:10.1016/j.vetpar.2021.109495

Cited by 3 publications

(6 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various antigens have been proposed for the diagnosis of bovine trypanosomiasis (20–24). Fleming et al (2016) identified two ISGs (TvY486_0019690 and TvY486_0045500) in African strain of T. vivax , with TvY486_0045500 showing the best diagnostic performance.…”

Section: Discussionmentioning

confidence: 99%

“…Cutoff values of the tests were 0.80 (indicated by broken lines). Samples from cattle infected with Babesia bovis (20), Anaplasma marginale (20), and Trypanosoma theileri (8).…”

Section: Supporting Informationmentioning

confidence: 99%

“…Assays employing recombinant proteins have eliminated the need for live parasite preparation. Different antigens have been proposed for the diagnosis of bovine trypanosomiasis, including MyxoTLm (20), GM6 (21,22), variant surface glycoproteins (VSG) (23) and invariant surface glycoproteins (ISGs) (24,25).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Improved Serodiagnosis ofTrypanosoma vivaxInfections in Cattle Reveals Higher Infection Rates in the Livestock Regions of Argentina

Bontempi,

Arias,

Castro

et al. 2024

Preprint

View full text Add to dashboard Cite

Bovine trypanosomiasis, caused by Trypanosoma vivax, currently affects cattle, resulting in significant economic consequences in sub-Saharan Africa and South America. The development of new diagnostic antigens is crucial for improving and refining existing methods. Our study assessed the effectiveness of two recombinant antigens in detecting specific antibodies in cattle. These antigens are derivatives of an invariant surface glycoprotein (ISG) from T. vivax. We evaluated a fraction of an antigen previously described (TvY486_0045500), referred to as TvISGAf, from an African strain, and identified a new ISG antigen from an American isolate, TvISGAm. ELISA evaluation using these antigens was conducted on 212 samples from cattle. The diagnostic performance was enhanced when utilizing a combination of both antigens (denominated TvISG-based ELISA), achieving a sensitivity of 89.6% and specificity of 93.8%. Following validation of the TvISG-based ELISA, we determined the seroprevalence of T. vivax infection in 892 field samples from cattle in the central region of Argentina. The average seroprevalence of T. vivax was 53%, with variation across the six surveyed departments ranging from 21% to 69%. These results support the use of the TvISG ELISA as a valuable serological tool for detecting and monitoring T. vivax infection in cattle. They also reported for the first time T. vivax seroprevalence in Argentina, highlighting the widespread endemic nature of the disease in the region. To effectively manage the increasing spread of T. vivax in the vast livestock production areas of South America, we support the need for consistent surveillance programs and implementation of preventive strategies.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Cutoff values of the tests were 0.80 (indicated by broken lines). Samples from cattle infected with Babesia bovis (20), Anaplasma marginale (20), and Trypanosoma theileri (8).…”

Section: Supporting Informationmentioning

confidence: 99%

See 1 more Smart Citation

Improved Serodiagnosis ofTrypanosoma vivaxInfections in Cattle Reveals Higher Infection Rates in the Livestock Regions of Argentina

Bontempi,

Arias,

Castro

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Several antigens have been proposed for the diagnosis of bovine trypanosomosis [20][21][22][23][24]. Fleming et al ( 2016) identified two ISGs (TvY486_0019690 and TvY486_0045500) from an African strain of T. vivax, with TvY486_0045500 demonstrating the most promising diagnostic performance [24].…”

Section: Discussionmentioning

confidence: 99%

“…Assays using recombinant proteins have eliminated the need for live parasite preparation. Several antigens have been proposed for the diagnosis of bovine trypanosomosis, including MyxoTLm [ 20 ], GM6 [ 21 , 22 ], variant surface glycoproteins (VSGs) [ 23 ] and invariant surface glycoproteins (ISGs) [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Improved serodiagnosis of Trypanosoma vivax infections in cattle reveals high infection rates in the livestock regions of Argentina

Bontempi,

Arias,

Castro

et al. 2024

PLoS Negl Trop Dis

View full text Add to dashboard Cite

Bovine trypanosomosis, caused by Trypanosoma vivax, currently affects cattle and has a significant economic impact in sub-Saharan Africa and South America. The development of new diagnostic antigens is essential to improve and refine existing methods. Our study evaluated the efficacy of two recombinant antigens in detecting specific antibodies in cattle. These antigens are derivatives of an invariant surface glycoprotein (ISG) from T. vivax. A fraction of a previously described antigen (TvY486_0045500), designated TvISGAf, from an African strain was evaluated, and a new ISG antigen from an American isolate, TvISGAm, was identified. The two antigens were expressed as fusion proteins in Escherichia coli: TvISGAf was fused to the MBP-His-tag, and TvISGAm was obtained as a His-tag fused protein. An ELISA evaluation was conducted using these antigens on 149 positive and 63 negative bovine samples. The diagnostic performance was enhanced by the use of a combination of both antigens (referred to as TvISG-based ELISA), achieving a sensitivity of 89.6% and specificity of 93.8%. Following the validation of the TvISG-based ELISA, the seroprevalence of T. vivax infection in 892 field samples from cattle in the central region of Argentina was determined. The mean seroprevalence of T. vivax was 53%, with variation ranging from 21% to 69% among the six departments studied. These results support the use of the TvISG ELISA as a valuable serological tool for the detection and monitoring of T. vivax infection in cattle. Furthermore, we report for the first time the seroprevalence of T. vivax in Argentina, which highlights the widespread endemic nature of the disease in the region. In order to effectively manage the increasing spread of T. vivax in the vast livestock production areas of South America, it is essential to implement consistent surveillance programs and to adopt preventive strategies.

show abstract

Methods Applied to the Diagnosis of Cattle Trypanosoma vivax Infection: An Overview of the Current State of the Art

Ferreira

Garcia

Araújo

et al. 2023

CPB

View full text Add to dashboard Cite

Trypanosoma vivax bovine trypanosomiasis is a relevant disease in domestic ungulates in Latin America, causing different types of livestock losses, particularly in African and South American countries, leading to loss of millions of dollars/year related to dairy and meat production. In addition, T. vivax trypanosomiasis requires intensive veterinary care. While vector control is a feasible measure to manage disease spreading, the search for accurate diagnostic tools still represents a gap in routine veterinary practices and a challenge for the scientific community. The parasite is mechanically transmitted by fomites or by the saliva of haematophagous, such as Stomoxys sp. and Tabanus sp., infecting cattle as well as a number of animal hosts. The main symptoms of T. vivax bovine trypanosomiasis are apathy, fever, restricted growth, miscarriage, progressive weakness, neurological signs, pale mucous, loss of appetite, lethargy, and substantial weight loss.In most cases, the presence of animals with subclinical infections, nonspecific symptoms and without apparent parasitaemia presents a challenge when making a diagnosis, which requires accurate methods. Herein, we review state of the art concerning current methods available for the diagnosis of T. vivax bovine trypanosomiasis, focusing on clinical, parasitological, immunological and molecular approaches, highlighting the main features of each method, including “pros and cons”. Overall, combining several diagnostic techniques is a better choice since it leads to fewer false negative results and contributes to better disease control.

show abstract

A recombinant protein (MyxoTLm) for the serological diagnosis of acute and chronic Trypanosoma vivax infection in cattle

Cited by 3 publications

References 24 publications

Improved Serodiagnosis ofTrypanosoma vivaxInfections in Cattle Reveals Higher Infection Rates in the Livestock Regions of Argentina

Improved Serodiagnosis ofTrypanosoma vivaxInfections in Cattle Reveals Higher Infection Rates in the Livestock Regions of Argentina

Improved serodiagnosis of Trypanosoma vivax infections in cattle reveals high infection rates in the livestock regions of Argentina

Methods Applied to the Diagnosis of Cattle Trypanosoma vivax Infection: An Overview of the Current State of the Art

Contact Info

Product

Resources

About