A recombinant DNA vaccine protects mice deficient in the alpha/beta interferon receptor against lethal challenge with Usutu virus

Martín-Acebes, Miguel A.; Blázquez, Ana-Belén; Cañas‐Arranz, Rodrigo; Vázquez-Calvo, Ángela; Merino-Ramos, Teresa; Escribano-Romero, Estela; Sánchez‐Madrid, Francisco; Saiz, Juan-Carlos

doi:10.1016/j.vaccine.2016.03.015

Cited by 35 publications

(42 citation statements)

References 46 publications

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“…The diagnosis of disease caused by USUV is further complicated by the resemblance of pathology to WNV cases and the serological cross-reactivity in diagnostic tests (11, 13, 18, 22–24, 97, 98). A mouse model for USUV infection has been recently established, and it represents a valuable tool to test the in vivo antiviral effect of 5-fluorouracil or novel antivirals (98, 99). These studies can be extended to the potential treatment of severe disease caused by closely related flaviviruses, such as WNV and Japanese encephalitis virus, for which antiviral therapies are not available.…”

Section: Discussionmentioning

confidence: 99%

Extinction of Zika Virus and Usutu Virus by Lethal Mutagenesis Reveals Different Patterns of Sensitivity to Three Mutagenic Drugs

Bassi

Sempere

Meyn

et al. 2018

Antimicrob Agents Chemother

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Flaviviruses constitute an increasing source of public health concern, with growing numbers of pathogens causing disease and geographic spread to temperate climates. Despite a large body of evidence supporting mutagenesis as a conceivable antiviral strategy, there are currently no data on the sensitivity to increased mutagenesis for Zika virus (ZIKV) and Usutu virus (USUV), two emerging flaviviral threats.

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Section: Discussionmentioning

confidence: 99%

Extinction of Zika Virus and Usutu Virus by Lethal Mutagenesis Reveals Different Patterns of Sensitivity to Three Mutagenic Drugs

Bassi

Sempere

Meyn

et al. 2018

Antimicrob Agents Chemother

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“…Similarly, wild-type mice showed nil or limited susceptibility when challenged with low or high doses of USUV via the intraperitoneal route (i.p.) [2,[21][22][23][24][25], including USUV prototype strain SAAR-1776 (GenBank: AY453412) [21,22,24], which was isolated by intracerebral inoculation of newborn mice [22]. However, in the study of Diagne et al [2], both subcutaneous and i.p.…”

Section: Introductionmentioning

confidence: 99%

New Insights into the Susceptibility of Immunocompetent Mice to Usutu Virus

Benzarti

Sarlet

Franssen

et al. 2020

Viruses

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Usutu virus (USUV) is a mosquito-borne flavivirus that shares many similarities with the closely related West Nile virus (WNV) in terms of ecology and clinical manifestations. Initially distributed in Africa, USUV emerged in Italy in 1996 and managed to co-circulate with WNV in many European countries in a similar mosquito–bird life cycle. The rapid geographic spread of USUV, the seasonal mass mortalities it causes in the European avifauna, and the increasing number of infections with neurological disease both in healthy and immunocompromised humans has stimulated interest in infection studies to delineate USUV pathogenesis. Here, we assessed the pathogenicity of two USUV isolates from a recent Belgian outbreak in immunocompetent mice. The intradermal injection of USUV gave rise to disorientation and paraplegia and was associated with neuronal death in the brain and spinal cord in a single mouse. Intranasal inoculation of USUV could also establish the infection; viral RNA was detected in the brain 15 days post-infection. Overall, this pilot study probes the suitability of this murine model for the study of USUV neuroinvasiveness and the possibility of direct transmission in mammals.

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“…A few research groups are currently working on developing laboratory animal models to study USUV virus. Adult mice are generally resistant to USUV infection but not the suckling mice . A recent study has shown that adult mice lacking interferon receptor alpha and beta are susceptible to USUV infections .…”

Section: Discussionmentioning

confidence: 99%

“…Adult mice are generally resistant to USUV infection but not the suckling mice . A recent study has shown that adult mice lacking interferon receptor alpha and beta are susceptible to USUV infections . The group also tested a DNA vaccine expressing the USUV envelope and pre‐M proteins and found that interferon receptor‐deficient adult mice could be protected against USUV infections .…”

Section: Discussionmentioning

confidence: 99%

Computational Prediction of Usutu Virus E Protein B Cell and T Cell Epitopes for Potential Vaccine Development

Palanisamy

Lennerstrand

2017

Scand J Immunol

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Usutu virus (family Flaviviridae), once confined to Africa, has emerged in Europe a decade ago. The virus has been spreading throughout Europe at a greater pace mostly affecting avian species. While most bird species remain asymptomatic carriers of this virus, few bird species are highly susceptible. Lately, Usutu virus (USUV) infections in humans were reported sporadically with severe neuroinvasive symptoms like meningoencephalitis. As so much is unknown about this virus, which potentially may cause severe diseases in humans, there is a need for more studies of this virus. In this study, we have used computational tools to predict potential B cell and T cell epitopes of USUV envelope (E) protein. We found that amino acids between positions 68 and 84 could be a potential B cell epitope, while amino acids between positions 53 and 69 could be a potential major histocompatibility complex (MHC) class I- and class II-restricted T cell epitope. By homology 3D modeling of USUV E protein, we found that the predicted B cell epitope was predominantly located in the coil region, while T cell epitope was located in the beta-strand region of the E protein. Additionally, the potential MHC class I T cell epitope (LAEVRSYCYL) was predicted to bind to nearly 24 human leucocyte antigens (HLAs) (IC ≤5000 nm) covering nearly 86.44% of the Black population and 96.90% of the Caucasoid population. Further in vivo studies are needed to validate the predicted epitopes.

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A recombinant DNA vaccine protects mice deficient in the alpha/beta interferon receptor against lethal challenge with Usutu virus

Cited by 35 publications

References 46 publications

Extinction of Zika Virus and Usutu Virus by Lethal Mutagenesis Reveals Different Patterns of Sensitivity to Three Mutagenic Drugs

Extinction of Zika Virus and Usutu Virus by Lethal Mutagenesis Reveals Different Patterns of Sensitivity to Three Mutagenic Drugs

New Insights into the Susceptibility of Immunocompetent Mice to Usutu Virus

Computational Prediction of Usutu Virus E Protein B Cell and T Cell Epitopes for Potential Vaccine Development

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