A recombinant-based ELISA evaluating the efficacy of netobimin and albendazole in ruminants with naturally acquired fascioliasis

Abstract

The therapeutic efficacy of albendazole and netobimin in ruminants with naturally occurring fascioliasis was investigated using a recombinant-based ELISA. The variation in the IgG response against a 2.9-kDa recombinant protein (FhrAPS), termed efficacy index (EI) 1, and the egg-output changes, termed EI 2, were used to evaluate drug efficacy. The values of EI 1 ranged between 0% and 50% in sheep, and between 0% and 30% in cattle after treatment with albendazole and netobimin. Similar EI 2 values were observed in sheep receiving albendazole or netobimin, but the highest values were found in cattle treated with netobimin. The significant reduction in the IgG response to FhrAPS found in this study shows promise in terms of developing alternative methods for evaluating the efficacy of chemotherapy against Fasciola hepatica in grazing ruminants.

Enzyme-linked immunosorbent assays for the detection of equine antibodies specific to a recombinant Fasciola hepatica surface antigen in an endemic area

“…There have been reported very low prevalences of natural infection by the analysis of faecal samples in some endemic areas of Chile (Apt et al 1993) and Egypt (Haridy et al 2002;Morsy et al 2005). An overall 60% of horses sensitized against the trematode was obtained, which is in agreement with previous works carried out on sheep and cattle in the same area (Paz-Silva et al 2003;Arias et al 2009). The foals reached the lowest values of seropositivity.…”

“…Five grams of each faecal sample was processed (in duplicate) by sedimentation (Arias et al 2009) with a sensitivity of 30 eggs per gram of faeces. The counts of trematode eggs were provided as counts of eggs per gram.…”

“…The values of seroprevalence oscillated between 65% and 92% (Sánchez-Andrade et al 2000Paz-Silva et al 2007;Arias et al 2009). …”

Prevalence of mixed trematode infections in an abattoir receiving cattle from northern Portugal and north‐west Spain

“…INFECTION of ruminants by the liver trematode parasite Fasciola hepatica is distributed worldwide, especially in areas under oceanic climate conditions (Arias and others 2009), where the presence of elevated humidity, frequent rainfall and mild temperatures provides a suitable environment for the development of its intermediate host, Galba truncatula (Rondelaud and others 2001, Phiri and others 2005). As Calicophoron daubneyi (Digenea: Paramphistomidae) (Dinnik 1962, Eduardo 1983) and F hepatica use the same lymnaeid ( G truncatula ) in their life cycle, the presence of mixed infections by liver and ruminant flukes has been reported both in the lymnaids and in the cattle (Abrous and others 1999, Dreyfuss and others 2008).…”

Fasciolosis in the Mediterranean island of Corsica (France): Insights from epidemiological and malacological investigations

“…Concerning those using flukicides, the frequency and timing of the treatment are factors as important as the type of treatment itself to take into account to achieve proper control of transmission. A variable efficacy against mature flukes have been reported for netobimin in the past ( Richards et al, 1987 ), while more recent studies in naturally-infected sheep recorded low or no change in F. hepatica egg outputs ( Sánchez-Andrade et al, 2001 ; Arias et al, 2009 ). Because in temperate regions transmission of F. hepatica follows a bi-seasonal pattern in summer and winter with prevalence in livestock increasing as the grazing season progresses ( Bloemhoff et al, 2015 ; Munita et al, 2019 ), it is particularly recommended to treat animals in late autumn and/or in winter, to reduce not only the parasite load in livestock but also the contamination of pastures with Fasciola eggs ( Fairweather et al, 2020 ).…”