A Recombinant Avian Leukosis Virus Subgroup J for Directly Monitoring Viral Infection and the Selection of Neutralizing Antibodies

Wang, Qi; Li, Xiaofei; Ji, Xiaolin; Wang, Jingfei; Shen, Nan; Gao, Yulong; Qi, Xiaole; Wang, Yongqiang; Gao, Honglei; Zhang, Shide; Wang, Xiaomei

doi:10.1371/journal.pone.0115422

Cited by 10 publications

(5 citation statements)

References 19 publications

(21 reference statements)

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“…Polymerase chain reaction (PCR) primers were employed to amplify the complete sequence, as described previously ( 16 ). Samples were examined by blood agglutination assay, PCR, or reverse transcription (RT) -PCR for the presence of DNA or RNA viruses, including avian reovirus ( 17 ), infectious bursal disease virus ( 18 ), reticuloendotheliosis virus ( 19 ), avian influenza virus ( 20 ), Newcastle disease virus ( 21 ), avian leukosis virus ( 22 ), and avian adenovirus 4 ( 23 ), according to previously described methods.…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China

DongDong

Liu

et al. 2022

Front. Vet. Sci.

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Budgerigar fledgling disease virus (BFDV) is the causative polyomavirus of budgerigar fledgling disease, an important avian immunosuppressive disease in budgerigars (Melopsittacus undulatus). In the current study, we explored the etiological role and molecular characteristics of BFDV. We identified a novel BFDV strain, designated as SC-YB19, belonging to a unique cluster with three other domestic strains (WF-GM01, SD18, and APV-P) and closely related to Polish isolates based on complete sequences. Sequence analysis showed that SC-YB19 had an 18-nucleotide (nt) deletion in the enhancer region, corresponding to the sequence position 164–181 nt, which differed significantly from all other BFDV strains. Based on sequence alignment, three unique nucleotide substitutions were found in VP4 (position 821), VP1 (position 2,383), and T-antigen (position 3,517) of SC-YB19, compared with SD18, WF-GM01, QDJM01, HBYM02, APV7, and BFDV1. Phylogenetic analyses based on complete sequences suggested that SC-YB19, along with the domestic WF-GM01, SD18, and APV-P strains, formed a single branch and were closely related to Polish, Japanese, and American isolates. These results demonstrate that BFDV genotype variations are co-circulating in China, thus providing important insight into BFDV evolution.

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Section: Methodsmentioning

confidence: 99%

Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China

DongDong

Liu

et al. 2022

Front. Vet. Sci.

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“…Depending on the humoral immune response and the presence of a detectable virus in the blood, susceptible and infected ALV chickens can be classified in several conventional categories: viremia with (V+A+) or without (V+A−) neutralizing antibodies, and no viremia with neutralizing antibodies (V−A+) [15,200,201]. Most of the infected chickens are V−A+.…”

Section: Humoral Immune Response: the Role Of Antibodiesmentioning

confidence: 99%

Avian Leukosis: Will We Be Able to Get Rid of It?

Fandiño,

Gomez-Lucia,

Benítez

et al. 2023

Animals

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Avian leukosis viruses (ALVs) have been virtually eradicated from commercial poultry. However, some niches remain as pockets from which this group of viruses may reemerge and induce economic losses. Such is the case of fancy, hobby, backyard chickens and indigenous or native breeds, which are not as strictly inspected as commercial poultry and which have been found to harbor ALVs. In addition, the genome of both poultry and of several gamebird species contain endogenous retroviral sequences. Circumstances that support keeping up surveillance include the detection of several ALV natural recombinants between exogenous and endogenous ALV-related sequences which, combined with the well-known ability of retroviruses to mutate, facilitate the emergence of escape mutants. The subgroup most prevalent nowadays, ALV-J, has emerged as a multi-recombinant which uses a different receptor from the previously known subgroups, greatly increasing its cell tropism and pathogenicity and making it more transmissible. In this review we describe the ALVs, their different subgroups and which receptor they use to infect the cell, their routes of transmission and their presence in different bird collectivities, and the immune response against them. We analyze the different systems to control them, from vaccination to the progress made editing the bird genome to generate mutated ALV receptors or selecting certain haplotypes.

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“…The PCR procedure consisted of an initial incubation for 5 min at 95°C; 35 cycles of 30 sec at 95°C, annealing for 60 sec at 55°C, and extension for 60 sec at 72°C; and a final extension for 5 min at 72°C. Samples were examined by blood agglutination assay, PCR, or RT-PCR for the presence of other DNA or RNA viruses, including avian reovirus (ARV) (Zhong et al, 2016), Marek's disease virus (MDV) (Lv et al, 2016), infectious bursal disease virus (IBDV) (Lu et al, 2015), reticuloendotheliosis virus (REV) (Jiang et al, 2013), avian influenza virus (AIV) (Li et al, 2006), Newcastle disease virus (NDV) (Zhu et al, 2016), and avian leukosis virus (ALV) (Wang et al, 2014), according to previously described methods.…”

Section: Nucleic Acid Extraction and Pcr Assaymentioning

confidence: 99%

The first whole genome sequence and pathogenicity characterization of a fowl adenovirus 4 isolated from ducks associated with inclusion body hepatitis and hydropericardium syndrome

et al. 2017

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In June 2015, an infectious disease with high prevalence causing severe hydropericardium syndrome (HPS) first appeared in duck farms of northeast China. The disease showed high morbidity of 35% and mortality of 15% in a commercial duck farm with 200,000 45-day-old ducks. One strain of hypervirulent fowl adenovirus serotype 4 was identified and designated as HLJDAd15. The whole genome of the duck isolate was sequenced and found to contain the same large deletions as a genotype that has become prevalent in chickens in China recently, indicating that this disease might be transmitted from chickens to ducks. The pathogenicity of HLJDAd15 was evaluated in SPF chickens and ducks. The results showed that chickens were more susceptible to this new genotype of fowl adenovirus, and it was more difficult to infect ducks than chickens with the duck origin virus. Thus, it appears that this severe HPS in ducks is far more likely to have been transmitted from chickens to ducks than from ducks to ducks. Therefore, transmission from chickens to ducks constitutes a threat to the duck farming industry, and this transmission route is a very important consideration for the prevention and control of the new genotype of fowl adenovirus. This is the first whole genome sequence of a FAdV-4 isolated from ducks, and this information is important for understanding the molecular characteristics and evolution of aviadenoviruses. The potential risks of infection with this new hypervirulent FAdV-4 genotype in chickens and ducks urgently require an effective vaccine.

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A Recombinant Avian Leukosis Virus Subgroup J for Directly Monitoring Viral Infection and the Selection of Neutralizing Antibodies

Cited by 10 publications

References 19 publications

Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China

Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China

Avian Leukosis: Will We Be Able to Get Rid of It?

The first whole genome sequence and pathogenicity characterization of a fowl adenovirus 4 isolated from ducks associated with inclusion body hepatitis and hydropericardium syndrome

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