A recessive gene Cmpmr2F confers powdery mildew resistance in melon (Cucumis melo L.)

Zhang, Taifeng; Cui, Haonan; Luan, Feishi; Liu, Hongyu; Ding, Zhuo; Amanullah, Sikandar; Zhang, Manlin; Ma, Tingting; Gao, Peng

doi:10.1007/s00122-023-04269-2

Cited by 8 publications

(6 citation statements)

References 70 publications

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“…To analyze the effects of domestication on GhCBSX3A locus, we focused on the VW DI of 340 cultivars and 37 wild/landrace accessions harboring either the GhCBSX3A_R or GhCBSX3A_S allele (Zhang, Zhang, Gao, et al, 2023). Our analysis showed that the GhCBSX3A_R allele was more common in wild/landrace accessions (81 of 201 accessions), but it was less frequent in improved upland cotton (50 of 370 accessions) (Figure 6a).…”

Section: Ghcbsx3a_r Haplotype Experienced Bi-direction Selection Duri...mentioning

confidence: 96%

Promotion of apoplastic oxidative burst by artificially selected GhCBSX3A enhances Verticillium dahliae resistance in upland cotton

Zhang,

Yuan,

et al. 2024

The Plant Journal

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SUMMARYVerticillium wilt (VW) is a devasting disease affecting various plants, including upland cotton, a crucial fiber crop. Despite its impact, the genetic basis underlying cotton's susceptibility or defense against VW remains unclear. Here, we conducted a genome‐wide association study on VW phenotyping in upland cotton and identified a locus on A13 that is significantly associated with VW resistance. We then identified a cystathionine β‐synthase domain gene at A13 locus, GhCBSX3A, which was induced by Verticillium dahliae. Functional analysis, including expression silencing in cotton and overexpression in Arabidopsis thaliana, confirmed that GhCBSX3A is a causal gene at the A13 locus, enhancing SAR‐RBOHs‐mediated apoplastic oxidative burst. We found allelic variation on the TATA‐box of GhCBSX3A promoter attenuated its expression in upland cotton, thereby weakening VW resistance. Interestingly, we discovered that altered artificial selection of GhCBSX3A_R (an elite allele for VW) under different VW pressures during domestication and other improved processes allows specific human needs to be met. Our findings underscore the importance of GhCBSX3A in response to VW, and we propose a model for defense‐associated genes being selected depending on the pathogen's pressure. The identified locus and gene serve as promising targets for VW resistance enhancement in cotton through genetic engineering.

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Section: Ghcbsx3a_r Haplotype Experienced Bi-direction Selection Duri...mentioning

confidence: 96%

Promotion of apoplastic oxidative burst by artificially selected GhCBSX3A enhances Verticillium dahliae resistance in upland cotton

Zhang,

Yuan,

et al. 2024

The Plant Journal

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“…The gene MELO3C004311, which encodes TMV resistance protein et al 2024). The recessive resistance gene for race 2F "Cmpmr2F" (MELO3C002403), encoding allantoate amidohydrolase, was identified on chromosome 12 (Zhang et al 2023a). FM: FM is incited by the fungal pathogen Fusarium oxysporum Schlechtend f. sp.…”

Section: Resistance To Other Diseasesmentioning

confidence: 99%

Possibility of genome editing for melon breeding

Nonaka,

Ezura

2024

Breed. Sci.

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Genome editing technologies are promising for conventional mutagenesis breeding, which takes a long time to remove unnecessary mutations through backcrossing and create new lines because they directly modify the target genes of elite strains. In particular, this technology has advantages for traits caused by the loss of function. Many efforts have been made to utilize this technique to introduce valuable features into crops, including maize, soybeans, and tomatoes. Several genome-edited crops have already been commercialized in the US and Japan. Melons are an important vegetable crop worldwide, produced and used in various areas. Therefore, many breeding efforts have been made to improve its fruit quality, resistance to plant diseases, and stress tolerance. Quantitative trait loci (QTL) analysis was performed, and various genes related to important traits were identified. Recently, several studies have shown that the CRISPR/Cas9 system can be applied to melons, resulting in its possible utilization as a breeding technique. Focusing on two productivity-related traits, disease resistance, and fruit quality, this review introduces the progress in genetics, examples of melon breeding through genome editing, improvements required for breeding applications, and the possibilities of genome editing in melon breeding.

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“…The cloning of the MLO genes sequences was performed for PM-resistant lines (MR-1 and PI124112) and PM-susceptible lines (X055 and Topmark), as earlier reported method (Zhang et al, 2023). The information of primer sequences for gene clone can be seen in Supplementary Table S1.…”

Section: Mlo Genes Cloning In Melonmentioning

confidence: 99%

Genome-wide identification, evolution, and expression analysis of MLO gene family in melon (Cucumis melo L.)

Zhang

Amanullah

et al. 2023

Front. Plant Sci.

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Powdery mildew (PM) is one of the main fungal diseases that appear during the cultivation of the melon fruit crop. Mildew Resistance Locus “O” (MLO) is known as a gene family and has seven conserved transmembrane domains. An induced functional loss of a specific MLO gene could mainly confer PM resistance to melons. However, the genomic structure of MLO genes and its main role in PM resistance still remain unclear in melon. In this study, bioinformatic analysis identified a total of 14 MLO gene family members in the melon genome sequence, and these genes were distributed in an uneven manner on eight chromosomes. The phylogenetic analysis divided the CmMLO genes into five different clades, and gene structural analysis showed that genes in the same clade had similar intron and exon distribution patterns. In addition, by cloning the CmMLO gene sequence in four melon lines, analyzing the CmMLO gene expression pattern after infection, and making microscopic observations of the infection pattern of PM, we concluded that the CmMLO5 (MELO3C012438) gene plays a negative role in regulating PM-resistance in the susceptible melon line (Topmark), and the critical time point for gene function was noticed at 24 and 72 hours after PM infection. The mutational analysis exhibited a single base mutation at 572 bp, which further results in loss of protein function, thus conferring PM resistance in melon. In summary, our research evidence provides a thorough understanding of the CmMLO gene family and demonstrates their potential role in disease resistance, as well as a theoretical foundation for melon disease resistance breeding.

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A recessive gene Cmpmr2F confers powdery mildew resistance in melon (Cucumis melo L.)

Cited by 8 publications

References 70 publications

Promotion of apoplastic oxidative burst by artificially selected GhCBSX3A enhances Verticillium dahliae resistance in upland cotton

Promotion of apoplastic oxidative burst by artificially selected GhCBSX3A enhances Verticillium dahliae resistance in upland cotton

Possibility of genome editing for melon breeding

Genome-wide identification, evolution, and expression analysis of MLO gene family in melon (Cucumis melo L.)

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