Annually resolved fossil records of nitrogen (N) inputs (as sedimentary d 15 N, N content), aquatic production (d 13 C, C content), and algal abundance and gross community composition (pigments, nonsiliceous microfossils) from Lough Neagh, Northern Ireland (NI), were compared with annual records of climatic variability, atmospheric and urban nutrient loading, whole-catchment nutrient budgets, and limnological monitoring data to identify the unique effects of N on the eutrophication of a phosphorus (P)-rich lake during ca. 1933-1995. Cluster analysis revealed two major biostratigraphic zones. Zone I (ca. 1933-1955) was characterized by moderate lake production, as inferred from low concentrations of most fossil pigments and reduced d 15 N signatures but elevated d 13 C values and chlorophyte microfossil concentrations. In contrast, Zone II (ca. 1955II (ca. -1995 exhibited greatly increased contents of 15 N, N, C, and algal pigments, combined with strongly reduced d 13 C ratios and chlorophyte fossil abundance, a pattern consistent with recent severe eutrophication. Overall, microfossils of diazotrophic cyanobacteria were most abundant during the transition period between zones (ca. 1955-1964). Regression analysis revealed that past N influx to the lake (as d 15 N; r 2 5 0.916, p , 0.0001), colonial cyanobacterial abundance (as myxoxanthophyll; r 2 5 0.837, p , 0.0001), and total algal standing crops (as b-carotene; r 2 5 0.388, p , 0.0001) were all strongly correlated to agricultural inputs of N to NI farmland, weakly correlated to P inputs to NI farmland (r 2 d15N 5 0.503, p , 0.0001; r 2 cyanobacteria 5 0.296, p , 0.0001; r 2 total algae 5 0.046, p . 0.05), and uncorrelated to most measures of climatic variability and atmospheric or urban nutrient inputs. Thus, degradation of water quality during the 20th century resulted from excessive loading of diffuse N to the lake from P-rich agricultural lands.