A real-time PCR analyzer compatible with high-throughput automated processing of 2-/spl mu/L reactions in glass capillaries

Ngatchou, P.N.; Holl, Mark R.; Fisher, C. H.; Saini, Mohan S.; Dong, Jianchun; Ren, Tao; Pence, William H.; Cunningham, D.L.; Moody, Stephen E.; Donaldson, D.A.; Meldrum, Deirdre R.

doi:10.1109/tase.2006.871479

Cited by 11 publications

(6 citation statements)

References 25 publications

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“…Heating and cooling operation is managed by a thermoelectric module named Peltier. [2][3][4] The amplification of DNA samples is performed in Thermal Cycler through multiple temperature cycles, with each cycle consisting of three preset temperatures: high (typically 92°C-96°C), low (typically 45°C-65°C) and medium (typically 68°C-74°C). Then, this desirable temperature-time path is given to machine as an input by biologist, which will be tracked by Peltier thermal function.…”

Section: Introductionmentioning

confidence: 99%

Optimization of nonlinear controller design for thermal management in polymerase chain reaction amplification

Nia

Kharrati

2014

Proceedings of the Institution of Mechanical Engineers, Part I:

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In this study, a new optimized nonlinear controller is designed for heat transfer part of a polymerase chain reaction machine (Thermal Cycler). Polymerase chain reaction is an important molecular technique which is performed in a Thermal Cycler to amplify a specific region of DNA. The main part of Thermal Cycler is a Peltier, which raises and lowers the temperature of the block in discrete, pre-programmed steps in several cycles. Based on the theoretical model, the goal was achieved using genetic algorithm in extended feedback linearization control system. The new optimized control structure was verified with improved temperature-tracking performance compared to earlier designs on reduced overshoot (0.3°C) and settling time (3.5-4.5 s faster). Also, the validation of the proposed method has been performed through simulation.

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Section: Introductionmentioning

confidence: 99%

Optimization of nonlinear controller design for thermal management in polymerase chain reaction amplification

Nia

Kharrati

2014

Proceedings of the Institution of Mechanical Engineers, Part I:

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“…Its usage is often considered as premium where their performance in rapid heating and cooling outweigh cost and inefficiencies in the areas such as DNA thermal cyclers, modern polymerase chain reaction (PCR) and alike [1]- [4]. This lack of application is partly due to the unavailability of high efficiency thermoelectric materials, and partly due to high power requirement of biomedical devices.…”

Section: Introductionmentioning

confidence: 99%

“…For a maximum thermoelectric device efficiency, the thermoelectric material been used must have high zT value [4]. The zT is given by: ( 1 ) where is the Seebeck coefficient; T is the temperature difference between the hot and cold terminal; is the electrical resistivity of the material being used to connect between two junctions; and k is the thermal conductivity of the material. To achieve high energy conversion efficiency, thermoelectric device must have high Seebeck coefficient while the electrical resistivity and thermal conductivity must be kept at minimum.…”

Section: Introductionmentioning

confidence: 99%

The effect of aluminum nanoparticle on the seebeck coefficient of biomedical thermoelectric devices

Azaddin

Ayub

Azman

et al. 2015

2015 IEEE Regional Symposium on Micro and Nanoelectronics (RSM)

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The Seebeck coefficient is a parameter to measure the efficiency of materials in generating thermoelectric voltage from certain temperature gradient. In this work, the effect of aluminum nano particle on the Seebeck coefficient for silicon based thermoelectric power generator is investigated. Thermoelectric devices which consist of arrays of doped phosphorus and boron channels have been fabricated on silicon wafers via standard CMOS fabrication processes. Aluminum nano particles were then integrated into the doped n-channel, before the fabrication step is completed with the metalization process. Temperature gradients across the two device terminals were created by resistive heating and the output voltages were measured. The results show that there is a substantial improvement in the calculated Seebeck coefficient where the device which been incorporated with 40 nm aluminum nano particles yields the average value of 3390 V/K to that of control device which yields 784 V/K. Furthermore, the output voltage is measured to be in the range of 40 mV for the temperature gradient of 12 K, making it to be very viable and attractive for biomedical applications.

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“…A stroke volume ΔV of reagent will be pumped out. (d) A peristaltic micro-pump actuated and controlled via the periodic generation of electrolytic bubbles [42]. The indirect air bubble pushes the solution from inlet to outlet.…”

mentioning

confidence: 99%

“…Most peristaltic micro-pumps are fabricated from polydimethylsiloxane (PDMS)with an external pneumatic source for actuation[20, 39]. Chiu et al developed a peristaltic micro-pump that is actuated and controlled via the periodic generation of electrolytic bubbles[42] (Figure 1.3(d)). The indirect air bubble pushes the solution from inlet to outlet.…”

mentioning

confidence: 99%

Microfluidic devices for gene preparation and detection

Xu¹

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First and foremost, I would like to thank my supervisor, Professor Jackie Y. Ying, for her close guidance, encouragement and scientific direction. This research would not have been possible without her help. Professor Ying was not only a supervisor, but also a mentor who gave me great support during the difficulties faced throughout my Ph.D. research. I am grateful to Ms Noreena AbuBakar; she provided me the privilege of working in a world-class research institute. Thanks also to Dr Mo-Huang Li for his ideas and suggestions during the course of my thesis research. The funding of Institute of Bioengineering and Nanotechnology (IBN) (Biomedical Research Council, Agency for Science and Technology and Research, Singapore) and the Ph.D. scholarship under the IBN Scientific Staff Development Scheme are much appreciated.

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A real-time PCR analyzer compatible with high-throughput automated processing of 2-/spl mu/L reactions in glass capillaries

Cited by 11 publications

References 25 publications

Optimization of nonlinear controller design for thermal management in polymerase chain reaction amplification

Optimization of nonlinear controller design for thermal management in polymerase chain reaction amplification

The effect of aluminum nanoparticle on the seebeck coefficient of biomedical thermoelectric devices

Microfluidic devices for gene preparation and detection

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