A reagentless nitric oxide biosensor based on haemoglobin/polyethyleneimine film

Abstract

An unmediated NO biosensor was prepared by co-immobilizing haemoglobin with polyethyleneimine at a pyrolytic graphite electrode surface. Haemoglobin exhibits direct electrochemistry in the membrane, and this modified electrode shows high stability and catalytic activity towards the reduction of NO. A linear relationship between the catalytic current and the concentration of NO was observed in the concentration ranges 1.0 x 10(-7)-8.0 x 10(-6) M and 1.0 x 10(-5)-2.5 x 10(-5) M, with a detection limit of 4.0 x 10(-8) M.

Nanomaterials-based electrochemical sensors for nitric oxide

“…The sensitive sensing system is also well-designed to further improve the analytical performance of NO sensor. There are two major channels for the design of NO sensitive surface, one is to employ redox mediator, such as macrocyclic metal complexes [31][32][33][34][35][36][37][38], watersoluble dyes [39][40][41][42][43] or metalloproteins [44][45][46][47][48][49], and the other is to introduce nanomaterials for the construction of the sensing interface.…”

Third-Generation Biosensors Based on the Direct Electron Transfer of Proteins

“…93 Hb incorporated in other polyelectrolytes, such as polyethyleneimine (PEI) films, also demonstrated nice catalytic activities for NO detection. 94 PC is a component of a biological membrane, and provides a mimic environment for the functioning of proteins and enzymes. A NO biosensor based on Hb incorporated in a PC film and immobilized at a pyrolytic graphite electrode has also been developed with the linear range of 0.1 -300 µM and a detection limit of 0.1 µM.…”

Electron transfer and interfacial behavior of redox proteins

“…Therefore, polyethyleneimine (PEI), polyethylene glycol (PEG) and poly-3-hydroxybutyrate (PHB) have all been used in this lab for the studies of ET and interfacial behavior of redox proteins, although PEI is highly positively charged, while the latter two are neutral. PEI is demonstrated to be able to facilitate the ET of heme proteins [55] and useful for other proteins as well, such as glucose oxidase (GOD). GOD is a structurally rigid glycoprotein with the molecular weight of 152000-186000 Da, which consists of two identical subunits, each containing a FAD prosthetic group.…”