A reagentless nitric oxide biosensor based on the direct electrochemistry of hemoglobin adsorbed on the gold colloids modified carbon paste electrode

Xu, Yanxia; Hu, Chengguo

doi:10.1016/j.snb.2010.05.028

Cited by 32 publications

(15 citation statements)

References 38 publications

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“…In addition, the substrate diffusion should take place easily. The employed approaches for preparation of third‐generation biosensors for NO detection (Table ) are mainly based on adsorption (physical and layer‐by‐layer ), entrapment or covalent bonding . Each method possesses its particular benefits and disadvantages.…”

Section: Direct Electron Transfer Behavior Of Heme Proteins and Porphmentioning

confidence: 99%

“…Each method possesses its particular benefits and disadvantages. Adsorption have been mostly used due to its simplicity and because loss of activity is not significant . However, leaching of the biocomponent has been reported as a problem due to the weak Van der Waal′s forces and electrostatic or hydrophobic interactions established .…”

Section: Direct Electron Transfer Behavior Of Heme Proteins and Porphmentioning

confidence: 99%

“…The choice of the working electrode is also crucial and several aspects, such as appropriate potential window, easy surface renewal, fast redox processes, reproducibility, easy miniaturization, low cost, the immobilized molecule and the substrate, should be considered. The privileged working electrodes have been glassy carbon (41 %) (GCE) and graphite pyrolytic (PGE) (31 %) electrodes, although gold electrodes (AuE) (9 %), powder microelectrode (PME) , carbon paste electrodes (CPE) , SnO 2 and TiO 2 electrodes , platinum (Pt) microelectrode and carbon fiber microelectrodes (CFM) have been also explored but in a much lesser extend (Table ).…”

Section: Direct Electron Transfer Behavior Of Heme Proteins and Porphmentioning

confidence: 99%

“…Xu et al. developed a novel biosensor based on Hb adsorbed on gold colloids modified carbon paste electrode (Hb/Au‐CPE) with a E°′ of −0.224 V vs. SCE . Guo et al.…”

Section: Direct Electron Transfer Behavior Of Heme Proteins and Porphmentioning

confidence: 99%

“… reported ‐0.351 V vs. SCE for a modified PME (Hb/didodecyldimethylammonium bromide (DDAB)/PME i.e. a similar E°′ as some GCE and PGE based transducers . PME can be a promising electrode because it can contribute to decrease interferences from capacitive currents and uncompensated iR .…”

Section: Direct Electron Transfer Behavior Of Heme Proteins and Porphmentioning

confidence: 99%

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Nitric Oxide Detection Using Electrochemical Third‐generation Biosensors – Based on Heme Proteins and Porphyrins

et al. 2018

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Nitric oxide radical (NO) is a signalling molecule involved in virtually all forms of life. Its relevance has been leading to the development of different analytical methodologies to assess the temporal and spatial fluxes of NO under the complex biological milieu. Third‐generation electrochemical biosensors are promising tools for in loco and in vivo NO quantification and, over the past years, heme proteins and porphyrins have been used in their design. Since there are some limitations with the biorecognition element directly adsorbed onto the electrode surface, nanomaterials (carbon nanotubes, gold nanoparticles, etc.) and polymers (cellulose, chitosan, nafion®, polyacrylamide, among others) have been explored to achieve high kinetics and better biosensor performance. In this review, a broad overview of the field of electrochemical third‐generation biosensors for NO electroanalysis is presented, discussing their main characteristics and aiming new outlooks and advances in this field.

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Section: Direct Electron Transfer Behavior Of Heme Proteins and Porphmentioning

confidence: 99%

Section: Direct Electron Transfer Behavior Of Heme Proteins and Porphmentioning

confidence: 99%

Section: Direct Electron Transfer Behavior Of Heme Proteins and Porphmentioning

confidence: 99%

“…Xu et al. developed a novel biosensor based on Hb adsorbed on gold colloids modified carbon paste electrode (Hb/Au‐CPE) with a E°′ of −0.224 V vs. SCE . Guo et al.…”

Section: Direct Electron Transfer Behavior Of Heme Proteins and Porphmentioning

confidence: 99%

Section: Direct Electron Transfer Behavior Of Heme Proteins and Porphmentioning

confidence: 99%

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Nitric Oxide Detection Using Electrochemical Third‐generation Biosensors – Based on Heme Proteins and Porphyrins

et al. 2018

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Recent advances in graphite powder-based electrodes

et al. 2013

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Graphite powder-based electrodes have the electrochemical performance of quasi-noble metal electrodes with intrinsic advantages related to the possibility of modification to enhance selectivity and their easily renewable surface, with no need for hazardous acids or bases for their cleaning. In contrast with commercial electrodes, for example screen-printed or sputtered-chip electrodes, graphite powder-based electrodes can also be fabricated in any laboratory with the form and characteristics desired. They are also readily modified with advanced materials, with relatively high reproducibility. All these characteristics make them a very interesting option for obtaining a large variety of electrodes to resolve different kinds of analytical problems. This review summarizes the state-of-the-art, advantages, and disadvantages of graphite powder-based electrodes in electrochemical analysis in the 21st century. It includes recent trends in carbon paste electrodes, devoting special attention to the use of emergent materials as new binders and to the development of other composite electrodes. The most recent advances in the use of graphite powder-modified sol-gel electrodes are also described. The development of sonogel-carbon electrodes and their use in electrochemical sensors and biosensors is included. These materials extend the possibilities of applications, especially for industrial technology-transfer purposes, and their development could affect not only electroanalytical green chemistry but other interesting areas also, for example catalysis and energy conversion and storage.

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Direct electrochemistry and electrocatalysis of hemoglobin on a glassy carbon electrode modified with poly(ethylene glycol diglycidyl ether) and gold nanoparticles on a quaternized cellulose support. A sensor for hydrogen peroxide and nitric oxide

Nie

et al. 2014

Microchim Acta

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A reagentless nitric oxide biosensor based on the direct electrochemistry of hemoglobin adsorbed on the gold colloids modified carbon paste electrode

Cited by 32 publications

References 38 publications

Nitric Oxide Detection Using Electrochemical Third‐generation Biosensors – Based on Heme Proteins and Porphyrins

Nitric Oxide Detection Using Electrochemical Third‐generation Biosensors – Based on Heme Proteins and Porphyrins

Recent advances in graphite powder-based electrodes

Direct electrochemistry and electrocatalysis of hemoglobin on a glassy carbon electrode modified with poly(ethylene glycol diglycidyl ether) and gold nanoparticles on a quaternized cellulose support. A sensor for hydrogen peroxide and nitric oxide

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