A reagentless aptasensor based on intrinsic aptamer redox activity for the detection of tetracycline in water

Alawad, Ahmad; Istamboulié, Georges; Calas‐Blanchard, Carole; Noguer, Thierry

doi:10.1016/j.snb.2019.02.103

Cited by 42 publications

(27 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figure 2‐b shows the mapping of the electrode surface after its modification by 4‐carboxyphenyl layer (4‐CP) which was achieved by the reduction of the corresponding diazonium salt. This latter step led to a decrease in the measured current down to values close to 0 pA over the scanning area, consistent with the passivation of the carbon surface occurring after diazonium grafting [8, 9]. After immobilization of anti‐TET aptamer by carbodiimide coupling, a new SECM experiment was performed.…”

Section: Figuresupporting

confidence: 58%

“…The sensing layer involved a specific anti‐TET aptamer consisting of a 76‐mer DNA oligonucleotide [10] that was immobilized by carbodiimide coupling on a screen‐printed carbon electrode (SPCE). Cyclic voltammetry experiments carried out for sensor characterization revealed a quasi‐reversible signal, with oxidation and reduction peaks located at +0.19 and −0.25 V vs silver/silver chloride (Ag/AgCl) reference electrode, respectively [9]. This very unusual and unexpected signal has been attributed to the intrinsic redox activity of the anti‐TET aptamer.…”

Section: Figurementioning

confidence: 96%

“…These results confirmed that green and red spots in figure 2‐c and 3‐a were due to the proper immobilization of the electroactive anti‐TET aptamer on the SPCE surface. Our previous study using the TET aptasensor demonstrated that the aptamer electrochemical response decreased in presence of TET [9], probably due to a conformational change upon target binding. In order to confirm this observation, we evaluated the effect of TET binding using SECM by scanning the sensing area after one hour of incubation with 45 nM of TET.…”

Section: Figurementioning

confidence: 96%

“…Among various aptasensors reported in literature, electrochemical aptasensors have been demonstrated as rapid, sensitive, selective and low‐cost devices [7, 8]. We have recently described the development of a label‐free electrochemical aptasensor for the reagentless determination of tetracycline (TET) antibiotic in water [9]. The sensing layer involved a specific anti‐TET aptamer consisting of a 76‐mer DNA oligonucleotide [10] that was immobilized by carbodiimide coupling on a screen‐printed carbon electrode (SPCE).…”

Section: Figurementioning

confidence: 99%

“…Further experiments showed a specific decrease of this signal in the presence of TET, demonstrating its proper binding by the aptamer. This remarkable behavior was exploited to detect TET at concentrations ranging from 0.11 to 45 nM, with a calculated limit of detection of 0.078 nM [9]. In the present work, we used scanning electrochemical microscopy (SECM) as an imaging technique to confirm the proper immobilization of intrinsically active anti‐TET aptamers and to evaluate their repartition on the surface of a SPCE‐based sensor.…”

Section: Figurementioning

confidence: 99%

See 4 more Smart Citations

SECM for Studying the Immobilization and Repartition of a Redox Anti‐tetracycline Aptamer on Screen‐printed Carbon Electrodes

et al. 2020

Self Cite

View full text Add to dashboard Cite

Scanning electrochemical microscopy (SECM) is discussed as a versatile tool to provide a unique approach of localized electrochemical information in the context of biosensing research. The step‐by‐step immobilization of DNA aptamer with intrinsic redox activity on screen‐printed carbon electrode (SPCE) was successfully monitored using SECM imaging tool. Control experiments were performed with a non‐electroactive aptamer. After immobilization of these aptamers, SECM images showed the repartition of the electroactive anti‐tetracycline aptamer when comparing with images produced for control and for all modification steps of SPCE. The possibility of tetracycline detection was also proved by causing a decrease in recorded current.

show abstract

Section: Figuresupporting

confidence: 58%

Section: Figurementioning

confidence: 96%

Section: Figurementioning

confidence: 96%

Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

See 3 more Smart Citations

SECM for Studying the Immobilization and Repartition of a Redox Anti‐tetracycline Aptamer on Screen‐printed Carbon Electrodes

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

Electrochemical aptasensors for pathogenic detection toward point‐of‐care diagnostics

Kızılkurtlu

Demirbaş

Ağel

2023

Biotech and App Biochem

View full text Add to dashboard Cite

A biosensor system refers to a biomedical device, which detects biological, chemical, or biochemical components by converting those signals to an electrical signal by utilizing and uniting physical or chemical transducer with biorecognition elements. An electrochemical biosensor is generally based on the reaction of either production or consumption of electrons under a three‐electrode system. Biosensor systems are exploited in a wide range of areas, such as medicine, agriculture, husbandry, food, industry, environment protection, quality control, waste disposal, and the military. Pathogenic infections are the third leading cause of death worldwide after cardiovascular diseases and cancer. Therefore, there is an urgent need for effective diagnostic tools to control food, water, and soil contamination result in protecting human life and health. Aptamers are peptide or oligonucleotide‐based molecules that show very high affinity to their targets that are produced from large pools of random amino acid or oligonucleotide sequences. Generally, aptamers have been utilized for fundamental sciences and clinical implementations for their target‐specific affinity and have been intensely exploited for different kinds of biosensor applications for approximately 30 years. The convergence of aptamers with biosensor systems enabled the construction of voltammetric, amperometric, and impedimetric biosensors for the detection of specific pathogens. In this review, electrochemical aptamer biosensors were evaluated by discussing the definition, types, and production techniques of aptamers, the advantages of aptamers as a biological recognition element against their alternatives, and a wide range of aptasensor examples from literature in the detection of specific pathogens.

show abstract

Synthesis and Characterization of Bis‐1,2,3‐Triazole Ligand and its Corresponding Copper Complex for the Development of Electrochemical Affinity Biosensors

Kanso

Jrad

Inguimbert

et al. 2021

Chemistry A European J

Self Cite

View full text Add to dashboard Cite

The bis‐triazole ligand and its corresponding copper complexes were synthesized and characterized for the first time and proposed as new labels for the development of electrochemical aptasensors. The bis‐triazole ligand was prepared from methyl 1,6‐heptadiyne‐4‐carboxylate and 2‐(azidomethyl)phenol using classical CuAAC in presence of different copper salts. The X‐ray structure of bis‐triazole showed a symmetry center (C1). UV‐Vis and X‐band EPR spectra showed that the coordination capacity of the bis‐triazole ligand was improved in the presence of triethylamine due to deprotonation of the triazole and phenolate moieties. After complexation with copper, the obtained complex was successfully attached to an anti‐estradiol aptamer through thiol‐maleimide coupling, and the resulting labelled aptamer was immobilized on a carbon screen‐printed electrode by carbodiimide coupling. The electrochemical response of the resulting sensor was shown to decrease in the presence of estradiol, demonstrating that the developed complexes can be applied for the development of aptasensors.

show abstract

A reagentless aptasensor based on intrinsic aptamer redox activity for the detection of tetracycline in water

Cited by 42 publications

References 43 publications

SECM for Studying the Immobilization and Repartition of a Redox Anti‐tetracycline Aptamer on Screen‐printed Carbon Electrodes

SECM for Studying the Immobilization and Repartition of a Redox Anti‐tetracycline Aptamer on Screen‐printed Carbon Electrodes

Electrochemical aptasensors for pathogenic detection toward point‐of‐care diagnostics

Synthesis and Characterization of Bis‐1,2,3‐Triazole Ligand and its Corresponding Copper Complex for the Development of Electrochemical Affinity Biosensors

Contact Info

Product

Resources

About