A re-usable wave bioreactor for protein production in insect cells

Scholz, Jochen; Suppmann, Sabine

doi:10.1016/j.mex.2016.08.001

Cited by 11 publications

(2 citation statements)

References 9 publications

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“…Batch cultivation of mammalian, insect, and plant cells have been reported in WAVE Bioreactors. 36,37 In this article, we have compared the WAVE bioreactor with static cultivation and summarized the implementation scheme used in both cultivation methods.…”

Section: Discussionmentioning

confidence: 99%

Rapid expansion in the WAVE bioreactor of clinical scale cells for tumor immunotherapy

Meng

Sun

et al. 2018

Human Vaccines & Immunotherapeutics

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Cell-based immunotherapy using natural killer (NK) cells, cytokine-induced killer (CIK) cells and dendritic cells (DCs) is emerging as a potential novel approach in the auxiliary treatment of a tumor. However, non-standard operation procedure, small-scale cell number, or human error may limit the clinical development of cell-based immunotherapy. To simplify clinical scale NK cells, CIK cells and DCs expansions, we investigated the use of the WAVE bioreactor, a closed system bioreactor that utilizes active perfusion to generate high cell numbers in minimal volumes. We developed an optimized rapid expansion protocol for the WAVE bioreactor that produces clinically relevant number of cells for our adoptive cell transfer clinical protocols. The high proliferative rate, surface phenotypes, and cytotoxicity of these immune cells, as well as the safety of cultivation were analyzed to illuminate the effect of WAVE bioreactor. The results demonstrated that the benefit of utilizing modern WAVE bioreactors in cancer immunotherapy was simple, safe, and flexible production.

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Section: Discussionmentioning

confidence: 99%

Rapid expansion in the WAVE bioreactor of clinical scale cells for tumor immunotherapy

Meng

Sun

et al. 2018

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

show abstract

“…However, for academic research or for cultivations that do not conform to GMP, re-usable options may be promising, e.g. the polycarbonate bioreactor proposed by Scholz and Suppmann [64].…”

Section: Wave-mixed Bag Bioreactormentioning

confidence: 99%