A rational approach for generating cardiac troponin I selective Spiegelmers

Szeitner, Zsuzsanna; Lautner, Gergely; Nagy, Szilvia K.; Gyurcsányi, Róbert E.; Mészáros, Tamás

doi:10.1039/c4cc00447g

Cited by 18 publications

(15 citation statements)

References 23 publications

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“…We have focused in particular on surface imprinting technologies to overcome the limitations imposed by the small diffusivity of proteins in the highly cross-linked MIP structures, by generating binding sites exclusively on the surface of the MIPs (Menaker et al, 2009) and simultaneously providing high surface area/volume MIP micro-or nanostructures (Bognár et al, 2013;Ceolin et al, 2013;Lautner et al, 2011). However, the development of protein MIPs would have to benefit clearly from high throughput synthesis and testing methodologies as commonly used in development of various bioreceptors, e.g., aptamers (Szeitner et al, 2014). Therefore, here we propose microelectrospotting as a new methodology to prepare by electrosynthesis arrays of surface-imprinted polymers for protein recognition on bare gold surface plasmon resonance imaging (SPRi) chips.…”

Section: Introductionmentioning

confidence: 99%

Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition

Bosserdt

Erdőssy²,

Lautner

et al. 2015

Biosensors and Bioelectronics

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Here we introduce microelectrospotting as a new approach for preparation of protein-selective molecularly imprinted polymer microarrays on bare gold SPR imaging chips. During electrospotting both the gold chip and the spotting tip are electrically connected to a potentiostat as working and counter electrodes, respectively. The spotting pin encloses the monomer-template protein cocktail that upon contacting the gold surface is in-situ electropolymerized resulting in surface confined polymer spots of ca. 500 µm diameter. By repeating this procedure at preprogrammed locations for various composition monomer-template mixtures microarrays of nanometer-thin surface-imprinted films are generated in a controlled manner. We show that the removal and rebinding kinetics of the template and various potential interferents to such microarrays can be monitored in real-time and multiplexed manner by SPR imaging. The proof of principle for microelectrospotting of electrically insulating surface-imprinted films is made by using scopoletin as monomer and ferritin as protein template. It is shown that microelectrospotting in combination with SPR imaging can offer a versatile platform for label-free and enhanced throughput optimization of the molecularly imprinted polymers for protein recognition and for their analytical application.

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Section: Introductionmentioning

confidence: 99%

Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition

Bosserdt

Erdőssy²,

Lautner

et al. 2015

Biosensors and Bioelectronics

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“…The resulting L-aptamers, called Spiegelmers, have shown good resistance to nuclease degradation, and, as a result, they have been selected to target a variety of small molecules, including gonadotropin-releasing hormone [60], ghrelin [61], and more recently, proteins such as cardiac troponin I [62].…”

Section: Selection Of Stable Aptamersmentioning

confidence: 99%

The Clinical Application of Aptamers: Future Challenges and Prospects

Song

Zhang

Zhu

et al. 2015

Aptamers Selected by Cell-Selex for Theranostics

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This final chapter attempts to search for reasons to explain why so little progress has been made in the practical clinical application of aptamers and propose potential solutions to the problem. The advantages and limitations of aptamers in clinical settings are first carefully evaluated. It is suggested that in order to increase the clinical application of aptamers, new selection methods are needed to further improve the success rate of aptamer selection and to efficiently generate stable aptamers for in vivo application with low cost. Several new and promising aptamer selection methods are then reviewed. Strategies for improving selection success rate are highlighted. Finally, efforts leading to the selection of stable aptamers and, hence, increasing the potential for the practical use of aptamer-based technology in clinical settings, are discussed.Keywords Aptamer Á Base modification Á Spiegelmer Á Microfluidics Á SELEX IntroductionBased on their unique functions and features, as discussed throughout the chapters of this book, aptamers have been developed over the past 25 years for applications in chemical sensing, clinical diagnosis, targeted drug delivery, and therapy [1][2][3][4][5][6]. Moreover, the utility of aptamers has been amplified by their compatibility with such detection schemes as electrochemistry [7], fluorescence [8,9], colorimetrics [6,[10][11][12], chemiluminescence [13], field effect transistors [14], and surface plasmon resonance (SPR) [15,16]. However, while aptamer development has flourished in the laboratory, the commercialization of aptamers, especially for biomedical applications, has lagged far behind. Since the invention of aptamer

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“…Furthermore, the custom synthesis of nucleic acid aptamers enables their chemical modification for finely tuned physical chemical properties, stabilization and increased functionality. In particular, the in vivo stability and the half-life of aptamers can be enhanced by the chemical modification of nucleotides [ 92 ] or conjugation of molecules like polyethylene glycol (PEG) [ 93 ]. The integration of reporter groups like fluorescent dyes or biotin at well-defined sites of the aptamer makes them useful in various sensing applications.…”

Section: Preparation and Performance Of Aptamers For The Recognitimentioning

confidence: 99%

MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing

et al. 2016

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Biomimetic binders and catalysts have been generated in order to substitute the biological pendants in separation techniques and bioanalysis. The two major approaches use either “evolution in the test tube” of nucleotides for the preparation of aptamers or total chemical synthesis for molecularly imprinted polymers (MIPs). The reproducible production of aptamers is a clear advantage, whilst the preparation of MIPs typically leads to a population of polymers with different binding sites. The realization of binding sites in the total bulk of the MIPs results in a higher binding capacity, however, on the expense of the accessibility and exchange rate. Furthermore, the readout of the bound analyte is easier for aptamers since the integration of signal generating labels is well established. On the other hand, the overall negative charge of the nucleotides makes aptamers prone to non-specific adsorption of positively charged constituents of the sample and the “biological” degradation of non-modified aptamers and ionic strength-dependent changes of conformation may be challenging in some application.

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A rational approach for generating cardiac troponin I selective Spiegelmers

Cited by 18 publications

References 23 publications

Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition

Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition

The Clinical Application of Aptamers: Future Challenges and Prospects

MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing

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