A rational and iterative process for targeted nanoparticle design and validation

Abstract

The lack of understanding of fundamental nano-bio interactions, and difficulties in designing particles stable in complex biological environments are major limitations to their translation into biomedical clinical applications. Here we present a multi-parametric approach to fully characterize targeted nanoparticles, and emphasizes the significant effect that each detail in the synthetic process can have on downstream in vitro results. Through an iterative process, particles were designed, synthesized and tested for physico-chemical and bio-interactive properties which allowed the optimization of nanoparticle functionality. Taken together all interative steps demonstrate that we have synthesized a multifunctional gold nanoparticles that can detect ERBB2-positive breast cancer cells while showing stealth-like behavior toward ERBB2-negative cells and excellent physicochemical stability.

Developing an improved optical biosensing system based on gold nanoparticles acting as interferometric enhancers in Lactoferrin detection

“…This demonstrated not only Au surface was efficiently coated with a dense PEG layer, but also the PEG chains presented a brush conformation as reported previously. 52 Then, a slight decrease in the hydrodynamic size was observed after Protein G conjugation, which could be attributed to the reduction of the electrostatic repulsion between the PEG chains in agreement with the zeta potential values (Table 1). Finally, an increase of ∼32 nm in the hydrodynamic size was recorded after antibody conjugation, which demonstrated that the antibody was efficiently conjugated since the typical size of the IgG is approx.…”

The Choice of Nanoparticle Surface‐Coupled Fluorescent Dyes Impacts Cellular Interaction

“…Upon functionalization, an expected increase in size of ∼20 nm was observed in PBS for the NPs (Table 1), indicating a PEG coating with a brush configuration as previously reported [11] . Furthermore, hydrodynamic diameters are comparable for all dye labelled and unlabelled (i. e. no dye, ND) PEGylated AuNPs.…”

Gold nanoparticles as cell regulators: beneficial effects of gold nanoparticles on the metabolic profile of mice with pre-existing obesity

“…receptor-mediated endocytosis or pinocytosis or phagocytosis or other) along with their subsequent intracellular fate [ 53 ]. As such, the initial nanoparticle synthesis itself ultimately dictates the downstream in vivo outcomes, as shown in a recent study by Rodriguez-Lorenzo et al [ 54 ].…”