A ratiometric near-infrared fluorescence strategy based on spiropyran in situ switching for tracking dynamic changes of live-cell lysosomal pH

Li, Jin; Li, Xiaokang; Jia, Jianbo; Chen, Xi; Lv, Yinjuan; Guo, Yuan; Li, Jian

doi:10.1016/j.dyepig.2019.03.060

Cited by 46 publications

(18 citation statements)

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“…Several lysosomal trackers have been developed and commercialized, such as LysoTracker Green (LTG), LysoTracker Red (LTR), and other LysoSensor dyes, − which greatly promoted the study of lysosomes. However, these LysoTrackers/Sensors have some obvious limitations, such as narrow Stokes shift (LTG, 7 nm; LTR, 13 nm), poor photostability, high background fluorescence staining, and high sensitivity to pH within the normal lysosomal pH range, which restricts them for long-time and high-fidelity lysosomal tracking. − To solve these problems, recently some new lysosomal probes based on nanoparticles, , polymers, metal complexes, aggregation-induced emission (AIE) molecules, − and others − have been reported one after another (some of them are shown in Table S1). However, some shortcomings also exist in these new probes, such as poor cell permeability, , difficult synthesis, poor biocompatibility, , and low water solubility. − With the deepening of lysosomal research, it is still very necessary to further develop new lysosomal probes, especially those with easy preparation, high photostability, long emission wavelength, high quantum yield, large Stokes shift, high-resolution imaging, and best use in a very low dose.…”

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confidence: 99%

Real-Time and High-Fidelity Tracking of Lysosomal Dynamics with a Dicyanoisophorone-Based Fluorescent Probe

Hong

Xia

2021

Anal. Chem.

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The development of high-performance probes that can visualize and track the dynamic changes of lysosomes is very important for the in-depth study of lysosomes. Herein, we report that a dicyanoisophorone-based probe (named DCIP) can be used for high-fidelity imaging of lysosomes and lysosomal dynamics. DCIP can be easily prepared and shows strong far-red to near-infrared emissions centered at 653 nm in water with a huge Stokes shift (224 nm), high quantum yield (Φ = 0.15), high pKa value (∼8.79), and good biocompatibility. DCIP also shows good cell permeability and can label lysosomes rapidly with bright fluorescence without a time-consuming washing process before imaging. DCIP also possesses good photostability and negligible background, making it effective for long-term and high spatiotemporal resolution (0.44 s of exposure) imaging of lysosomes. Moreover, DCIP achieved high-fidelity tracking of lysosomal dynamics at an extremely low concentration (1 nM). Finally, we also demonstrated that DCIP could real-time track the interactions of lysosomes with other organelles (damaged mitochondria as a model) and image the drug-escape processes from lysosomes. All of the results show that DCIP holds broad prospects in lysosome-related research.

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confidence: 99%

Real-Time and High-Fidelity Tracking of Lysosomal Dynamics with a Dicyanoisophorone-Based Fluorescent Probe

Hong

Xia

2021

Anal. Chem.

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“…and formylation [23,24] at position 8 and cyanation at position 4 [17] of 7-hydroxy-3-(benzothiazol-2-yl)coumarin were previously studied.…”

Section: -Benzothiazolylcoumarins Arementioning

confidence: 99%

Hetarenocoumarins based on 7-hydroxy-3-(benzothiazol-2-yl)coumarin

et al. 2021

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“…[10] In recent years, many excellent pH-dependent fluorescent probes with acidic response behaviours have been exploited to study biological systems. [12][13][14][15][16][17][18][19][20][21][22] However, the number of alkaline fluorescent pH probes is still relatively few. [11,[23][24][25][26] At present, a research hotspots is to develop efficient, near neutral to weakly alkaline, fluorescent pH probes.…”

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Ratiometric fluorimetric and colorimetric probe for sensing and imaging pH changes in living cells

et al. 2022

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Cell, enzyme, and tissue activity in living organisms are closely related to intracellular pH. Detecting the changes of intracellular pH is important to understanding the physiological and pathological changes in the process of crucial cell metabolism. A pH probe (HTBI) based on hemicyanine was synthesized. The probe solution displayed a marked colour change from yellow to amaranth with the pH increase from neutral to basic; simultaneously, the emission spectra showed a significant red shift. The probe exhibited a ratiometric fluorescence emission (F 586nm /F 542nm ) characteristic of pK a 8.82. As expected, HTBI exhibited high sensitivity and selectivity for pH, fine photostability, reversibility, and low cytotoxicity. Therefore, it would be a very useful tool for measuring the intracellular pH changes.

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A ratiometric near-infrared fluorescence strategy based on spiropyran in situ switching for tracking dynamic changes of live-cell lysosomal pH

Cited by 46 publications

References 54 publications

Real-Time and High-Fidelity Tracking of Lysosomal Dynamics with a Dicyanoisophorone-Based Fluorescent Probe

Real-Time and High-Fidelity Tracking of Lysosomal Dynamics with a Dicyanoisophorone-Based Fluorescent Probe

Hetarenocoumarins based on 7-hydroxy-3-(benzothiazol-2-yl)coumarin

Ratiometric fluorimetric and colorimetric probe for sensing and imaging pH changes in living cells

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