A ratiometric fluorescent probe for real-time monitoring of intracellular glutathione fluctuations in response to cisplatin

Abstract

Real-time imaging of fluctuations in intracellular glutathione (GSH) concentrations is critical to understanding the mechanism of GSH-related cisplatin-resistance. Here, we describe a ratiometric fluorescence probe based on a reversible Michael...

Probing Cell Redox State and Glutathione-Modulating Factors Using a Monochlorobimane-Based Microplate Assay

“…In spite of the unflagging interest in the development of new luminescence probes for GSH, such as bis-pyrene-Cu(II) complex [ 17 ], vinyl-functionalized boron-dipyrromethene (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) (BODIPY) [ 18 , 19 ] and sulfinyl naphthalimides [ 20 ], monochlorobimane (MCB) remains one of the most selective and available fluorescent dyes for this purpose. It has been widely used to assess physiological variations of GSH in different types of mammalian cells, mainly using sensitive fluorescence microscopy techniques [ 21 , 22 , 23 , 24 , 25 ].…”

Orthogonally aligned cyclic BODIPY arrays with long-lived triplet excited states as efficient heavy-atom-free photosensitizers

“…It features a rigid structure, easy accessibility, reasonably good stability and excellent photophysical properties, including strong photon capture ability which is easily tuneable from the visible to near infrared range. 4 c – g It also has rich chemistry and multiple sites (six pyrrolic, one meso - and one boron) for the facile incorporation of various functionalities at different positions of the BODIPY platform to meet a set of specific requirements for different applications, from fluorescence probes (for metal ions, 5 biomolecules, 6 viscosity, 7 polarity 8 and temperature 9 within living cells) to photosensitizers for photodynamic therapy (PDT). 10 Therefore, BODIPY is an appealing platform for the construction of chromophore arrays.…”

Reductase and Light Programmatical Gated DNA Nanodevice for Spatiotemporally Controlled Imaging of Biomolecules in Subcellular Organelles under Hypoxic Conditions

“…Owing to the unique advantages of high spatiotemporal resolution, sensitivity, and simplicity imparted by optical imaging, extensive efforts have been made in recent decades to develop various fluorescent probes for detection of intracellular biomolecules. − Since it can remain stable during circulation and easily be activated at desired sites of action, on-demand activated fluorescent probes with spatiotemporal resolution serve as a promising tool in basic research and therapeutic applications. − Light is the most attractive noninvasive regulatory entity because it can be controlled spatially and temporally without contaminating the reaction system. − Recently, many strategies have been proposed to design spatiotemporally gated fluorescent probes by incorporating photosensitive groups into DNA assembly to control cell function or affect biological pathways or gene expression. − For example, Li and co-workers reported a series of near-infrared (NIR) light-mediated, spatiotemporally controlled sensing technology for intracellular or subcellular organelle imaging and therapy. − However, some major challenges still exist. Since the probes used in previous reports lack specific targeting ability to organelles and can only sense biomolecules in organelles under normoxia, there is still much room for improvement in the study of spatiotemporally controlled imaging of biomolecules in subcellular organelles under hypoxic conditions.…”