A ratiometric fluorescence probe for the selective detection of H<sub>2</sub>S in serum using a pyrene-DPA–Cd<sup>2+</sup> complex

Kim, Jihoon; Oh, Jinyoung; Han, Min Su

doi:10.1039/d1ra04277g

Cited by 6 publications

(2 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From the perspective of practical sensors, the Turn-ON response is generally preferable because it can be clearly distinguished from the background emission. 24 In addition, because Cd is highly toxic, we selected Zn-dpa-C4Py as the best candidate for practical usage, and the feasibility of this probe was further investigated, as described below.…”

Section: Resultsmentioning

confidence: 99%

Simple and Rapid Endotoxin Recognition Using a Dipicolylamine-Modified Fluorescent Probe with Picomolar-Order Sensitivity

et al. 2022

View full text Add to dashboard Cite

Endotoxin is a lipopolysaccharide (LPS) that is found in the outer membrane of the cell wall of Gram-negative bacteria. Due to its high toxicity, the allowable endotoxin limit for water for injection is set at a very low value. Conventional methods for endotoxin detection are time-consuming and expensive and have low reproducibility. A previous study has shown that dipicolylamine ( dpa )-modified pyrene-based probes exhibit fluorescence enhancement in response to LPS; however, the application of such probes to the sensing of LPS is not discussed. Against this backdrop, we have developed a simple and rapid endotoxin detection method using a dpa -modified pyrenyl probe having a zinc(II) center ( Zn-dpa-C4Py ). When LPS was added into Zn-dpa-C4Py solution, excimer emission of the pyrene moiety emerged at 470 nm. This probe can detect picomolar concentrations of LPS (limit of detection = 41 pM). The high sensitivity of the probe is ascribed to the electrostatic and hydrophobic interactions between the probe and LPS, which result in the dimer formation of the pyrene moieties. We also found that Zn-dpa-C4Py has the highest selectivity for LPS compared with other phosphate derivatives, which is probably caused by the co-aggregation of the probe with LPS. We propose that Zn-dpa-C4Py is a promising chemical sensor for the detection of endotoxin in medical and pharmaceutical applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Simple and Rapid Endotoxin Recognition Using a Dipicolylamine-Modified Fluorescent Probe with Picomolar-Order Sensitivity

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The electron-withdrawing group becomes converted into an electron donor group, which leads to the spectral shift [78]. The pyrene-containing probes responding by H 2 S-induced monomer-excimer transition were also suggested [79]. The λ-ratiometric probes for H 2 S detection become available for the near-IR region [80].…”

Section: Signaling Molecules In Living Cellsmentioning

confidence: 99%

Dual emission and its λ-ratiometric detection in analytical fluorimetry Pt. II. Exploration in sensing and imaging

Demchenko

2023

Methods Appl. Fluoresc.

View full text Add to dashboard Cite

The wavelength-ratiometric techniques demonstrate strong advantages in fluorescence sensing and imaging over techniques employing variations of intensity at single wavelength. We present different possibilities for realization of these advantages in different simplified, miniaturized and multiplexing devices. They include the smartphone-based detection systems and strips, in which the color changes are observed with naked eye. The array-based techniques and different immunoassays with λ-ratiometric detection demonstrate strongly increased stability and sensitivity. The application areas extend from on-site monitoring of environment and point-of-care diagnostics to testing in personal need. Selected examples of sensing different analytes in chemical and biological systems demonstrate multiple possibilities of coupling the analyte-sensor interaction with the generation of λ-ratiometric output signal. Among them, simultaneous detection of several analytes and performing logical operations that can be useful in analysis. Finally, the benefits of multicolor ratiometric fluorescence imaging are demonstrated by visualization the functionally important parameters of biological membranes.

show abstract

Progress on the reaction-based methods for detection of endogenous hydrogen sulfide

et al. 2021

View full text Add to dashboard Cite

A ratiometric fluorescence probe for the selective detection of H₂S in serum using a pyrene-DPA–Cd²⁺ complex

Abstract: The pyrene-DPA–Cd2+ complex is demonstrated as a ratiometric fluorescence probe for selective hydrogen sulfide detection in serum based on a metal displacement approach.

Cited by 6 publications

References 35 publications

Simple and Rapid Endotoxin Recognition Using a Dipicolylamine-Modified Fluorescent Probe with Picomolar-Order Sensitivity

Simple and Rapid Endotoxin Recognition Using a Dipicolylamine-Modified Fluorescent Probe with Picomolar-Order Sensitivity

Dual emission and its λ-ratiometric detection in analytical fluorimetry Pt. II. Exploration in sensing and imaging

Progress on the reaction-based methods for detection of endogenous hydrogen sulfide

Contact Info

Product

Resources

About

A ratiometric fluorescence probe for the selective detection of H2S in serum using a pyrene-DPA–Cd2+ complex

Abstract: The pyrene-DPA–Cd2+ complex is demonstrated as a ratiometric fluorescence probe for selective hydrogen sulfide detection in serum based on a metal displacement approach.

Cited by 6 publications

References 35 publications

Simple and Rapid Endotoxin Recognition Using a Dipicolylamine-Modified Fluorescent Probe with Picomolar-Order Sensitivity

Simple and Rapid Endotoxin Recognition Using a Dipicolylamine-Modified Fluorescent Probe with Picomolar-Order Sensitivity

Dual emission and its λ-ratiometric detection in analytical fluorimetry Pt. II. Exploration in sensing and imaging

Progress on the reaction-based methods for detection of endogenous hydrogen sulfide

Contact Info

Product

Resources

About

A ratiometric fluorescence probe for the selective detection of H₂S in serum using a pyrene-DPA–Cd²⁺ complex