A ratiometric electrochemical DNA biosensor for detection of exosomal MicroRNA

Luo, Li-Pei; Wang, Liangliang; Zeng, Lupeng; Wang, Yuru; Weng, Yun-Ping; Liao, Yijuan; Chen, Tingting; Xia, Yaokun; Zhang, Jing; Chen, Jinghua

doi:10.1016/j.talanta.2019.120298

Cited by 77 publications

(47 citation statements)

References 39 publications

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“…The biosensor exhibited high specificity with a negligible response obtained for single‐ and double‐mismatched RNA sequences. The device exhibited high assay accuracy for the analysis of miRNA‐21 released from exosomes produced by a MCF‐7 cell line, as was confirmed by a reference analytical method …”

Section: Electrochemical Exosome Biosensingmentioning

confidence: 58%

“…In addition to CD63, quite a number of other exosomal proteins were targeted for biosensing purposes involving exosome‐surface‐confined proteins like EpCAM, CD24, CA125, HER2, MUC18, PSMA, FAM134B, GPC1, AFP, CEA, CD9 or exosomal phosphatidylserine (Table ). A typical cancer biomarker detected in the intra‐exosomal space (exosome interior i. e. exosome cargo) was miRNA‐21 and only one protein to date . In order to detect biomarkers from the intra‐exosomal space, detergents are usually used, but there is a possibility to induce lysis of vesicles using an electric field, making it possible to integrate lysis and the analysis of biomarkers present in the intra‐exosomal space even without surfactants.…”

Section: Discussionmentioning

confidence: 99%

“…Luo et al . developed a ratiometric biosensor device for the detection of miRNA‐21 isolated from exosomes (90–130 nm) . GCE was modified by a polylysine film, to which a DNA probe 1 labelled with MB was covalently attached, followed by hybridisation with a DNA probe 2 labelled with ferrocene (Fc).…”

Section: Electrochemical Exosome Biosensingmentioning

confidence: 99%

“… Methods for microvesicle isolation and selective for exosomal miRNA‐21 detection. Reprinted from, Copyright (2020), with permission from Elsevier.…”

Section: Electrochemical Exosome Biosensingmentioning

confidence: 99%

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Exosomes as a Source of Cancer Biomarkers: Advances in Electrochemical Biosensing of Exosomes

et al. 2020

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Exosomes are naturally produced biological nanoparticles secreted by cells into body fluids and responsible for intercellular communication. Exosomes can also carry cargo affecting neighbouring cells and forming pre‐metastatic niches. Hence, exosomes are behind localised tumour development, progression, but also the induction of distant tumours forming metastasis. A substantially higher cellular activity of tumour cells results in the production of a greater number of exosomes than in normal, healthy cells. Therefore, the number of exosomes present in body fluids can serve as a good diagnostic biomarker in itself, besides the presence of other tumour biomarkers, which can be substantially enriched in exosomes compared to parental cells. This review comprehensively summarises the development of electrochemical biosensors for the detection of exosome levels, exosome‐derived cancer biomarkers in cell lines and serum samples for the better diagnosis of various cancer types. The review provides information on the design and analytical parameters of such biosensors. The clinical utility of the level of exosomes or exosome‐derived biomarkers is also provided.

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Section: Electrochemical Exosome Biosensingmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Electrochemical Exosome Biosensingmentioning

confidence: 99%

“… Methods for microvesicle isolation and selective for exosomal miRNA‐21 detection. Reprinted from, Copyright (2020), with permission from Elsevier.…”

Section: Electrochemical Exosome Biosensingmentioning

confidence: 99%

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Exosomes as a Source of Cancer Biomarkers: Advances in Electrochemical Biosensing of Exosomes

et al. 2020

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“…Zhou and colleagues [ 46 ] studied a series of patients with ESCC and reported that two exosomal miRNAs, miR-223-3p and miR-584, may be satisfactorily used in disease detection. As novel methods are being developed to study miRNAs [ 47 , 48 ], further improvement in the diagnosis of ESCC will come, in part, from the detection of their exosomal population. Recent data lending further support for a role of EVs as a biomarker are provided by studies on patients with adenocarcinoma of the oesophagus [ 49 , 50 ].…”

Section: Evs In Gastrointestinal (Gi) and Liver Cancer Managementmentioning

confidence: 99%

EVs as Potential New Therapeutic Tool/Target in Gastrointestinal Cancer and HCC

Słomka

Mocan

Wang

et al. 2020

Cancers

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For more than a decade, extracellular vesicles (EVs) have been in focus of science. Once thought to be an efficient way to eliminate undesirable cell content, EVs are now well-accepted as being an important alternative to cytokines and chemokines in cell-to-cell communication route. With their cargos, mainly consisting of functional proteins, lipids and nucleic acids, they can activate signalling cascades and thus change the phenotype of recipient cells at local and systemic levels. Their substantial role as modulators of various physiological and pathological processes is acknowledged. Importantly, more and more evidence arises that EVs play a pivotal role in many stages of carcinogenesis. Via EV-mediated communication, tumour cells can manipulate cells from host immune system or from the tumour microenvironment, and, ultimately, they promote tumour progression and modulate host immunity towards tumour’s favour. Additionally, the role of EVs in modulating resistance to pharmacological and radiological therapy of many cancer types has become evident lately. Our understanding of EV biology and their role in cancer promotion and drug resistance has evolved considerably in recent years. In this review, we specifically discuss the current knowledge on the association between EVs and gastrointestinal (GI) and liver cancers, including their potential for diagnosis and treatment.

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Empowering Electrochemical Biosensing through Nanostructured or Multifunctional Nucleic Acid or Peptide Biomaterials

Campuzano

Pedrero

Barderas

et al. 2022

Adv Materials Technologies

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Electrochemical biosensors continue to evolve at an astonishing pace, consolidating as competitive tools for determining a wide range of targets and relentlessly strengthening their attributes in terms of sensitivity, selectivity, simplicity, response time, and antifouling ability, making them suitable for getting a foothold in real‐world applications. The design and exploitation of nanostructured or multifunctional nucleic acid or peptide biomaterials are playing a determinant role in these achievements. With the aim of highlighting the potential and opportunities of these biomaterials, this perspective article critically discusses and overviews the electrochemical biosensors reported since 2019 involving nanostructured and multifunctional DNA biomaterials, multifunctional aptamers, modern peptides, and CRISPR/Cas systems. The use of these biomaterials as recognition elements, electrode modifiers (acting as linkers or creating scaffolds with antifouling properties), enzyme substrates, and labeling/carrier agents for signal amplification is discussed through rationally and strategically selected examples, concluding with a personal perspective about the challenges to be faced and future lines of action.

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A ratiometric electrochemical DNA biosensor for detection of exosomal MicroRNA

Cited by 77 publications

References 39 publications

Exosomes as a Source of Cancer Biomarkers: Advances in Electrochemical Biosensing of Exosomes

Exosomes as a Source of Cancer Biomarkers: Advances in Electrochemical Biosensing of Exosomes

EVs as Potential New Therapeutic Tool/Target in Gastrointestinal Cancer and HCC

Empowering Electrochemical Biosensing through Nanostructured or Multifunctional Nucleic Acid or Peptide Biomaterials

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