A rat model of mild intestinal inflammation induced by<i>Staphylococcus aureus</i>enterotoxin B

Pérez-Bosque, Anna; Moretó, Miquel

doi:10.1017/s0029665110001849

Cited by 22 publications

(19 citation statements)

References 51 publications

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“…In mice, the SEB challenge induces intestinal inflammation with features that are similar to those previously described in the rat [12]. In both species, SEB increases infiltration of immune cells into intestinal mucosa and enhances production of pro-inflammatory cytokines, without affecting the histology of the jejunum or systemic (immune) variables.…”

Section: Discussionmentioning

confidence: 53%

“…The limited magnitude of the GALT response to the SEB challenge makes this model appropriate for the study of dietary approaches that may have modulatory effects on signaling pathways. As a result of immune activation, the expression of junctional proteins, such as zonula occludens-1 and β-catenin, is reduced, which is consistent with the increased in luminal water contents and mucosal permeability [12]. SEB also reduces the expression of mucosal defensins [13] and SGLT1 abundance in villous apex [14].…”

Section: Introductionmentioning

confidence: 85%

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The Anti-Inflammatory Effect of Spray-Dried Plasma Is Mediated by a Reduction in Mucosal Lymphocyte Activation and Infiltration in a Mouse Model of Intestinal Inflammation

et al. 2016

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Spray-dried preparations from porcine and bovine plasma can alleviate mucosal inflammation in experimental models and improve symptoms in patients with enteropathy. In rodents, dietary supplementation with porcine spray-dried plasma (SDP) attenuates intestinal inflammation and improves the epithelial barrier function during intestinal inflammation induced by Staphylococcus aureus enterotoxin B (SEB). The aim of this study was to discern the molecular mechanisms involved in the anti-inflammatory effects of SDP. Male C57BL/6 mice were fed with 8% SDP or control diet (based on milk proteins) for two weeks, from weaning until day 33. On day 32, the mice were given a SEB dose (i.p., 25 µg/mouse) or vehicle. SEB administration increased cell recruitment to mesenteric lymph nodes and the percentage of activated Th lymphocytes and SDP prevented these effects). SDP supplementation increased the expression of interleukin 10 (IL-10) or transforming growth factor- β (TGF-β) compared to the SEB group. The SEB challenge increased six-fold the expression of mucosal addressin cell adhesion molecule 1 (MAdCAM-1) and intercellular adhesion molecule 1 (ICAM-1); and these effects were attenuated by SDP supplementation. SEB also augmented NF-κB phosphorylation, an effect that was prevented by dietary SDP. Our results indicate that the anti-inflammatory effects of SDP involve the regulation of transcription factors and adhesion molecules that reduce intestinal cell infiltration and the degree of the inflammatory response.

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Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 85%

The Anti-Inflammatory Effect of Spray-Dried Plasma Is Mediated by a Reduction in Mucosal Lymphocyte Activation and Infiltration in a Mouse Model of Intestinal Inflammation

et al. 2016

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“…This therapeutic effect is fully manifested when experimental animals are exposed to bacteria and bacterial toxins (28). Immunoglobulin concentrates from animal plasma can reduce both the expression of mucosal proinflammatory cytokines (4) and the activation of Th subsets in lamina propria and epithelium (31). At the same time they can enhance the expression of anti-inflammatory cytokines (29), thereby contributing to the restoration of intestinal homeostasis.…”

Section: Fitc-dextranmentioning

confidence: 99%

Dietary intervention with serum-derived bovine immunoglobulins protects barrier function in a mouse model of colitis

Pérez-Bosque

Miró

Maijó

et al. 2015

American Journal of Physiology-Gastrointestinal and Liver Physi

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Dietary supplementation with immunoglobulins from animal plasma has anti-inflammatory effects on intestinal and lung models of acute inflammation. Here, we aimed to establish whether dietary intervention with serum-derived bovine immunoglobulin (SBI) can prevent alterations in intestinal barrier function in a mouse model with a genetic predisposition to inflammatory bowel disease (IBD). Wild-type (WT) mice and mice lacking the mdr1a gene (KO) were fed diets supplemented with either SBI (2% wt/wt) or milk proteins (control diet), from day 21 (weaning) until day 56. The epithelial permeability of distal colon crypts was measured by confocal microscopy using a fluorescent marker. The expression of junctional epithelial E-cadherin and β-catenin proteins were determined by Western blot and zonula occludens-1 (ZO-1) by immunofluorescence. Mucins (MUC1, MUC2, MUC4), TFF3, cytokines (TNF-α, IFN-γ), and inducible nitric oxide synthase RNA expression were quantified by real-time PCR. SBI blocked the increase in colon crypt permeability and partially prevented the reduction in E-cadherin and ZO-1 expression that characterize the KO mouse model (both P < 0.05). SBI inclusion also reduced the mucosal expression of the inflammatory markers TNF-α, IFN-γ, and inducible nitric oxide synthase (all P < 0.005). The number of goblet cells in the colon of KO mice was low and correlated well with MUC2 and TFF3 expression (P < 0.001), whereas dietary supplementation with SBI attenuated these effects (all P < 0.05). In short, dietary SBI ameliorated colonic barrier alterations and reduced the expression of mucosal inflammatory markers in a genetic model of IBD.

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“…The mechanism of action of the antiinflammatory effects of oral plasma proteins, though still controversial, involves the mucosal organized and diffuse GALT. In rats, administration of SEB produces mild intestinal inflammation (7) and this response is partially blocked by dietary supplementation with animal plasma proteins, either SDP or an Ig-enriched plasma fraction (IC) (8)(9)(10). In both cases, there is a reduction in the GALT mucosal response (11).…”

Section: Introductionmentioning

confidence: 99%

Dietary Plasma Proteins Modulate the Adaptive Immune Response in Mice with Acute Lung Inflammation

Maijó

Miró

Polo

et al. 2012

The Journal of Nutrition

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We examined the effects of oral plasma protein supplements on the pulmonary adaptive immune response in mice challenged with intranasal LPS. C57BL/6 mice were fed a control diet or a diet supplemented with plasma proteins [spray-dried plasma (SDP) 80 g/kg] or with an Ig concentrate [(IC) 20 g/kg] from postnatal d 19 (weaning) until d 34. Mice were challenged with PBS or LPS from Escherichia coli at d 33 and killed 24 h later for leukocyte analyses or at d 34 and killed 6 h later for cytokine determination. LPS induced the activation of T helper (Th) lymphocytes in lung and blood and this response was reduced by SDP and IC (P < 0.05). In both tissues, LPS increased the Th1 and Th2 subpopulations and this effect was inhibited by the two plasma protein supplements (P < 0.05). The LPS challenge increased the expression of all the cytokines studied (P < 0.01). SDP and IC reduced the expression of IFNγ, IL-5, IL-12p40, IL-12p70, IL-13, and IL-17 in both tissues, whereas they increased the percentage of regulatory Th lymphocytes in lung, even in PBS-treated mice (P < 0.05). LPS reduced the concentration of mature TGFβ1 (P < 0.05) in the lung but did not modify the expression of IL-10. Mice exposed to LPS and supplemented with SDP or IC showed an increased expression of the anti-inflammatory cytokine IL-10 (P < 0.05). Moreover, the two supplements increased the concentration of IL-10 in intestinal mucosa (P < 0.05). Our results show that plasma supplementation reduces the immune response that characterizes the acute lung inflammation syndrome.

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A rat model of mild intestinal inflammation induced byStaphylococcus aureusenterotoxin B

Cited by 22 publications

References 51 publications

The Anti-Inflammatory Effect of Spray-Dried Plasma Is Mediated by a Reduction in Mucosal Lymphocyte Activation and Infiltration in a Mouse Model of Intestinal Inflammation

The Anti-Inflammatory Effect of Spray-Dried Plasma Is Mediated by a Reduction in Mucosal Lymphocyte Activation and Infiltration in a Mouse Model of Intestinal Inflammation

Dietary intervention with serum-derived bovine immunoglobulins protects barrier function in a mouse model of colitis

Dietary Plasma Proteins Modulate the Adaptive Immune Response in Mice with Acute Lung Inflammation

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