A rat model for dose-response relationships of Salmonella Enteritidis infection

Havelaar, Arie H.; Garssen, Johan; Takumi, Katsuhisa; Koedam, M.A.; Dufrenne, J.; Leusden, F. M. Van; Fonteyne, Liset de la; Bousema, Teun; Vos, J.G.

doi:10.1046/j.1365-2672.2001.01399.x

Cited by 45 publications

(47 citation statements)

References 22 publications

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“…Enteritidis strains LA5 and S1400 colonized and invaded in a manner generally similar to that seen with other S. Enteritidis or S. Typhimurium strains (Naughton et al, 1995(Naughton et al, , 1996a(Naughton et al, , 2000Havelaar et al, 2001;Islam et al, 2000). Thus, there was rapid distribution throughout the gastrointestinal tract, translocation to the mesenteric lymph nodes and spread to the liver and spleen of the rats.…”

Section: Discussionmentioning

confidence: 58%

“…Salmonella sp. colonize the whole gastrointestinal tract of the rat and cause disruption of the small intestine epithelium, epithelial cell hyperplasia and infiltration by inflammatory cells (Naughton et al, 1995(Naughton et al, , 1996a(Naughton et al, , 2000Havelaar et al, 2001;Islam et al, 2000). Invasion is mainly via the ileum; large numbers of salmonella reach the mesenteric lymph nodes and moderate numbers spread to and persist in the liver and spleen.…”

Section: Introductionmentioning

confidence: 99%

“…Rats continue to grow, albeit slowly, and death due to infection is rare, unless health or immune status or gut integrity has been compromised by other factors. Salmonellosis in the rat thus has many similarities to the self-limiting gastroenteritis-type infection common in humans and domesticated animals (Naughton et al, 1995(Naughton et al, , 1996a(Naughton et al, , 2000Havelaar et al, 2001;Islam et al, 2000). Defined fimbriae-(SEF14, SEF17, SEF21, pef and lpf) and/or flagella-deleted mutants of S. Enteritidis and their wild-type parent strains were tested in this infection model.…”

Section: Introductionmentioning

confidence: 99%

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Lack of flagella disadvantages Salmonella enterica serovar Enteritidis during the early stages of infection in the rat

Robertson¹,

Duncan²,

Allen‐Vercoe³

et al. 2003

Journal of Medical Microbiology

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The roles of flagella and five fimbriae (SEF14, SEF17, SEF21, pef, lpf) in the early stages (up to 3 days) of Salmonella enterica serovar Enteritidis (S. Enteritidis) infection have been investigated in the rat. Wild-type strains LA5 and S1400 (fim+/fla+) and insertionally inactivated mutants unable to express the five fimbriae (fim2/fla+), flagella (fim+/fla2) or fimbriae and flagella (fim2/fla2) were used. All wild-type and mutant strains were able to colonize the gut and spread to the mesenteric lymph nodes, liver and spleen. There appeared to be little or no difference between the fim2/fla+ and wild-type (fim+/fla+) strains. In contrast, the numbers of aflagellate (fim+/fla2 or fim2/fla2) salmonella in the liver and spleen were transiently reduced. In addition, fim+/fla2 or fim2/fla2 strains were less able to persist in the upper gastrointestinal tract and the inflammatory responses they elicited in the gut were less severe. Thus, expression of SEF14, SEF17, SEF21, pef and lpf did not appear to be a prerequisite for induction of S. Enteritidis infection in the rat. Deletion of flagella did, however, disadvantage the bacterium. This may be due to the inability to produce or release the potent immunomodulating protein flagellin.

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Section: Discussionmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lack of flagella disadvantages Salmonella enterica serovar Enteritidis during the early stages of infection in the rat

Robertson¹,

Duncan²,

Allen‐Vercoe³

et al. 2003

Journal of Medical Microbiology

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“…However, many aspects, such as time-course effects of Salmonella colonization and translocation, are largely similar in this rat model compared with humans (3,5,15).…”

Section: Discussionmentioning

confidence: 88%

Gene expression response of the rat small intestine following oral Salmonella infection

Rodenburg

Bovee‐Oudenhoven

Kramer

et al. 2007

Physiological Genomics

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Data on the molecular response of the intestine to the food-borne pathogen Salmonella are derived from in vitro studies, whereas in vivo data are lacking. We performed an oral S. enteritidis infection study in Wistar rats to obtain insight in the in vivo response in time. Expression profiles of ileal mucosa (IM) and Peyer's patches (PP) were generated using DNA microarrays at days 1, 3, and 6 postinfection. An overview of Salmonella-regulated processes was obtained and confirmed by quantitative real-time PCR on pooled and individual samples. Salmonella-induced gene expression responses in vivo are fewer and smaller than observed in vitro, and the response develops over a longer period of time. Few effects are seen at day 1 and mainly occur in IM, suggesting the mucosa as the primary site of invasion. Later, a bigger response is observed, especially in PP. Decreased expression of anti-microbial peptides genes (in IM at day 1) suggests inhibition of this process by Salmonella. Newly identified target processes are carbohydrate transport (increased expression in IM at day 1) and phase I and phase II detoxification (decreased expression at days 3 and 6). Increase of cytokine and chemokine expression occurs at later time points, both in PP and IM. Pancreatitis-associated protein, lipocalin 2, and calprotectin, potential inflammatory marker proteins, showed induced expression from day 3 onward. We conclude that the in vivo gene expression response of the ileum to Salmonella differs to a large extent from the response seen in vitro.

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“…El bazo ha sido reportado como el órgano más sensible y útil para la detección de Salmonella enterica en infecciones sistémicas aislada en roedores (Havelaar et al, 2001 Además, se encontraron cepas resistentes a furazolidona (15.0%) y colistina (12.5%) entre otras. Por otro lado, el 42.5% de las cepas mostraron una susceptibilidad intermedia contra oxitetraciclina (Cuadro 1).…”

Section: Resultados Y Discusiónunclassified

SUSCEPTIBILIDAD A ANTIBACTERIANOS in vitro DE Salmonella enterica AISLADA DE CUYES DE CRIANZA FAMILIAR-COMERCIAL EN LA PROVINCIA DE CARHUAZ, ÁNCASH

et al. 2011

Rev. investig. vet. Perú

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RESUMENEl objetivo del presente estudio fue determinar la sensibilidad de Salmonella enterica aislada de cuyes, con signos clínicos sugerentes de salmonelosis, frente a 12 antibacterianos. Los cuyes fueron criados bajo un sistema familiar-comercial en la provincia de Carhuaz, Áncash, entre mayo a julio de 2007. Se colectó muestras de bazo, hígado y otros órganos con lesión aparente (n=65). Para el aislamiento de S. enterica se utilizaron métodos convencionales y el análisis de sensibilidad a antibacterianos fue mediante el método de difusión por discos de Kirby-Bauer. Cepas de S. enterica se aislaron en el 61.5% de los cuyes. El 100% de estas cepas (40/40) fue sensible a enrofloxacina, sulfatrimetoprim, estreptomicina y amoxicilina, el 97.5% al cloranfenicol y gentamicina, y el 92.5% a fosfomicina. Se encontraron cepas resistentes a furazolidona (15.0%) y colistina (12.5%) entre otras. Los resultados indican que la sensibilidad de S. enterica a enrofloxacina y sulfatrimetoprim, así como al cloranfenicol, gentamicina y fosfomicina, hacen de estos antibacterianos buenas alternativas para el tratamiento de la salmonelosis en las granjas estudiadas.

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A rat model for dose-response relationships of Salmonella Enteritidis infection

Cited by 45 publications

References 22 publications

Lack of flagella disadvantages Salmonella enterica serovar Enteritidis during the early stages of infection in the rat

Lack of flagella disadvantages Salmonella enterica serovar Enteritidis during the early stages of infection in the rat

Gene expression response of the rat small intestine following oral Salmonella infection

SUSCEPTIBILIDAD A ANTIBACTERIANOS in vitro DE Salmonella enterica AISLADA DE CUYES DE CRIANZA FAMILIAR-COMERCIAL EN LA PROVINCIA DE CARHUAZ, ÁNCASH

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