A rat mammary gland cancer cell with stem cell properties of self-renewal and multi-lineage differentiation

Abstract

The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing cancer-initiating cells with stem cell properties at the single cell level has proven technically difficult because of the scarcity of the cancer stem cells in the tissue of origin and the lack of specific markers for cancer stem cells. Here we show that a single LA7 cell, derived from rat mammary adenocarcinoma has: the ability to serially re-generate mammospheres in long-term non-adherent cultures, the differentiation potential to generate all the cell lineages of the mammary gland and branched duct-like structures that recapitulate morphologically and functionally the ductal-alveolar-like architecture of the mammary tree. The properties of self-renewal, extensive capacity for proliferation, multi-lineage differentiation and the tubular-like structure formation potential suggest that LA7 cells is a cancer stem model system to study the dynamics of tumor formation at the single cell level.

Chemopreventive Activity of Ferulago angulate against Breast Tumor in Rats and the Apoptotic Effect of Polycerasoidin in MCF7 Cells: A Bioassay-Guided Approach

“…LA7 is a mammary tumor cell line derived from a Sprague-Dawley adult female rat. This cell line is a clonal derivative of the Rama 25 line obtained from a rat treated with dimethylbenz(a)anthracene [ 30 , 31 ].…”

Identification of functionally related genes using data mining and data integration: a breast cancer case study

“…This type of analysis can be used to identify putative TBX3 protein interaction regulatory regions and enhancer sequences on chromatin that modulate cell function [ 23 ]. This assay was performed using two different cell lines, the LA7 cell line [ 24 - 26 ] and a well known human breast cancer cell line, BT474. Primer pairs were selected to amplify beta-actin which was used as a negative control for both cell lines (Figure 4A and Figure 4B , upper left and right panels), and GATA3 (Figure 4A , middle panel) or GLI-Kruppel family member (GLI3) (Figure 4A and Figure 4B , lower left and right panels) regions containing the potential binding sites for TBX3 protein.…”

Paclitaxel-Loaded Colloidal Silica and TPGS-Based Solid Self-Emulsifying System Interferes Akt/mTOR Pathway in MDA-MB-231 and Demonstrates Anti-tumor Effect in Syngeneic Mammary Tumors

“…For the in vivo anticancer studies, we have chosen syngeneic mammary tumor model in Sprague Dawley rats. The proposed model has various advantages, viz (i) single-cell tumor initiation and multi-lineage differentiation potential, (ii) prompt proliferation and self-renewal characteristics, (iii) transplantation ability, (iv) simplicity of tumor induction, and (v) their similarity in hormone sensitivity and histology to human breast cancer (38). As described in the experimental part after the development of the tumor, animals were treated with various dosage regimens and anti-tumor efficacy was evaluated.…”