A rapidly prototyped lung-on-a-chip model using 3D-printed molds

Shrestha, Jesus; Ghadiri, Maliheh; Shanmugavel, Melane; Bazaz, Sajad Razavi; Vasilescu, Steven; Ding, Lin; Warkiani, Majid Ebrahimi

doi:10.1016/j.ooc.2020.100001

Cited by 68 publications

(52 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inertial microfluidics exploits the interplay of focusing forces present within spiral microchannels, leveraging the variability in size and deformability of different cell types to perform high throughout cell separation. The microfluidic separation device used in this study was a 3D printed spiral microchannel, which was developed using previously reported fabrication methods 30 , 35 , 36 . This 3D printed microfluidic device not only allows for high throughout and efficient sperm recovery, but also presents new focusing behaviour for cells of a certain size, not seen in flexible PDMS microchannels.…”

Section: Resultsmentioning

confidence: 99%

A microfluidic approach to rapid sperm recovery from heterogeneous cell suspensions

Vasilescu

Khorsandi

Ding

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

The isolation of sperm cells from background cell populations and debris is an essential step in all assisted reproductive technologies. Conventional techniques for sperm recovery from testicular sperm extractions stagnate at the sample processing stage, where it can take several hours to identify viable sperm from a background of collateral cells such as white bloods cells (WBCs), red blood cells (RBCs), epithelial cells (ECs) and in some cases cancer cells. Manual identification of sperm from contaminating cells and debris is a tedious and time-consuming operation that can be suitably addressed through inertial microfluidics. Microfluidics has proven an effective technology for high-quality sperm selection based on motility. However, motility-based selection methods cannot cater for viable, non-motile sperm often present in testicular or epididymal sperm extractions and aspirations. This study demonstrates the use of a 3D printed inertial microfluidic device for the separation of sperm cells from a mixed suspension of WBCs, RBCs, ECs, and leukemic cancer cells. This technology presents a 36-fold time improvement for the recovery of sperm cells (> 96%) by separating sperm, RBCS, WBCs, ECs and cancer cells into tight bands in less than 5 min. Furthermore, microfluidic processing of sperm has no impact on sperm parameters; vitality, motility, morphology, or DNA fragmentation of sperm. Applying inertial microfluidics for non-motile sperm recovery can greatly improve the current processing procedure of testicular sperm extractions, simplifying the fertility outcomes for severe forms of male infertility that warrant the surgery.

show abstract

Section: Resultsmentioning

confidence: 99%

A microfluidic approach to rapid sperm recovery from heterogeneous cell suspensions

Vasilescu

Khorsandi

Ding

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Shrestha and co-workers [ 36 ] presented a protocol for surface modification of 3D-printed molds by DLP, in order to fabricate a lung-on-a-chip with high resolution by using PDMS casting. Initially, the 3D-printed mold is washed with isopropanol and then, after the print, by high-pressure air drying.…”

Section: Resultsmentioning

confidence: 99%

“…However, attention must be taken in the selection of the 3D printing technique to obtain molds for PDMS casting. For example, molds printed via SLA/DLP methods may not be appropriate for PDMS casting because residual oligomers and monomers on the top of the 3D-printed pieces hamper PDMS polymerization [ 36 ]. Hence, the development of optimized surface treatments is crucial for ensuring long-term cell viability in OoC devices.…”

Section: Resultsmentioning

confidence: 99%

3D Printing Techniques and Their Applications to Organ-on-a-Chip Platforms: A Systematic Review

Carvalho

Gonçalves

Lage

et al. 2021

Sensors

View full text Add to dashboard Cite

Three-dimensional (3D) in vitro models, such as organ-on-a-chip platforms, are an emerging and effective technology that allows the replication of the function of tissues and organs, bridging the gap amid the conventional models based on planar cell cultures or animals and the complex human system. Hence, they have been increasingly used for biomedical research, such as drug discovery and personalized healthcare. A promising strategy for their fabrication is 3D printing, a layer-by-layer fabrication process that allows the construction of complex 3D structures. In contrast, 3D bioprinting, an evolving biofabrication method, focuses on the accurate deposition of hydrogel bioinks loaded with cells to construct tissue-engineered structures. The purpose of the present work is to conduct a systematic review (SR) of the published literature, according to the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses, providing a source of information on the evolution of organ-on-a-chip platforms obtained resorting to 3D printing and bioprinting techniques. In the literature search, PubMed, Scopus, and ScienceDirect databases were used, and two authors independently performed the search, study selection, and data extraction. The goal of this SR is to highlight the importance and advantages of using 3D printing techniques in obtaining organ-on-a-chip platforms, and also to identify potential gaps and future perspectives in this research field. Additionally, challenges in integrating sensors in organs-on-chip platforms are briefly investigated and discussed.

show abstract

“…However, a laboratory model is still essential for airway phage therapy application and AEC ALI cultures still remain the most relevant model that mimics key features of the airway epithelium. Recent developments in 3D printing of organs is rapidly evolving and may be able to add further complexity whilst remaining accurate in its recapitulation of the human lung ( Grigoryan et al, 2019 ; Shrestha et al, 2019 ).…”

Section: The Potential Of Phage Therapy In Cfmentioning

confidence: 99%

Overcoming Challenges to Make Bacteriophage Therapy Standard Clinical Treatment Practice for Cystic Fibrosis

Tai

Chang

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Individuals with cystic fibrosis (CF) are given antimicrobials as prophylaxis against bacterial lung infection, which contributes to the growing emergence of multidrug resistant (MDR) pathogens isolated. Pathogens such as Pseudomonas aeruginosa that are commonly isolated from individuals with CF are armed with an arsenal of protective and virulence mechanisms, complicating eradication and treatment strategies. While translation of phage therapy into standard care for CF has been explored, challenges such as the lack of an appropriate animal model demonstrating safety in vivo exist. In this review, we have discussed and provided some insights in the use of primary airway epithelial cells to represent the mucoenvironment of the CF lungs to demonstrate safety and efficacy of phage therapy. The combination of phage therapy and antimicrobials is gaining attention and has the potential to delay the onset of MDR infections. It is evident that efforts to translate phage therapy into standard clinical practice have gained traction in the past 5 years. Ultimately, collaboration, transparency in data publications and standardized policies are needed for clinical translation.

show abstract

A rapidly prototyped lung-on-a-chip model using 3D-printed molds

Cited by 68 publications

References 52 publications

A microfluidic approach to rapid sperm recovery from heterogeneous cell suspensions

A microfluidic approach to rapid sperm recovery from heterogeneous cell suspensions

3D Printing Techniques and Their Applications to Organ-on-a-Chip Platforms: A Systematic Review

Overcoming Challenges to Make Bacteriophage Therapy Standard Clinical Treatment Practice for Cystic Fibrosis

Contact Info

Product

Resources

About