A Rapid Vascular Anastomosis Technique for Hind-Limb Transplantation in Rats

Sucher, Robert; Oberhuber, Rupert; Rumberg, Guido; Hautz, Theresa; Zelger, Bettina; Glodny, Bernhard; Jindal, Rishi; Pulikkottil, Benson J.; Gorantla, Vijay S.; Brandacher, Gerald; Margreiter, Raimund; Lee, W. P. Andrew; Schneeberger, Stefan

doi:10.1097/prs.0b013e3181e5fb11

Cited by 11 publications

(14 citation statements)

References 9 publications

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“…Microsurgical anastomosis of the femoral artery was performed with interrupted 11-0 nylon stitches, whereas a polyamide tube (RiverTech Medical, Chattanooga, TN) was used as cuff for the femoral vein, as reported earlier. 17…”

Section: Surgical Technique and Procedural Detailmentioning

confidence: 99%

Adipose- and Bone Marrow–Derived Mesenchymal Stem Cells Prolong Graft Survival in Vascularized Composite Allotransplantation

et al. 2015

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Section: Surgical Technique and Procedural Detailmentioning

confidence: 99%

Adipose- and Bone Marrow–Derived Mesenchymal Stem Cells Prolong Graft Survival in Vascularized Composite Allotransplantation

et al. 2015

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“…Recipient Lewis rats were similarly prepped, and the femoral vessels were dissected. The allograft was transplanted by using a polyimide cuff for venous anastomosis and 10‐0 nylon suture for the arterial anastomosis . The allograft was inset into the recipient groin area, and skin was closed using 3‐0 silk sutures.…”

Section: Methodsmentioning

confidence: 99%

Ex vivo allotransplantation engineering: Delivery of mesenchymal stem cells prolongs rejection-free allograft survival

Soares

Massie

Rifkin

et al. 2018

American Journal of Transplantation

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Current pharmacologic regimens in transplantation prevent allograft rejection through systemic recipient immunosuppression but are associated with severe morbidity and mortality. The ultimate goal of transplantation is the prevention of allograft rejection while maintaining recipient immunocompetence. We hypothesized that allografts could be engineered ex vivo (after allotransplant procurement but before transplantation) by using mesenchymal stem cell-based therapy to generate localized immunomodulation without affecting systemic recipient immunocompetence. To this end, we evaluated the therapeutic efficacy of bone marrow-derived mesenchymal stem cells in vitro and activated them toward an immunomodulatory fate by priming in inflammatory or hypoxic microenvironments. Using an established rat hindlimb model for allotransplantation, we were able to significantly prolong rejection-free allograft survival with a single perioperative ex vivo infusion of bone marrow-derived mesenchymal stem cells through the allograft vasculature, in the absence of long-term pharmacologic immunosuppression. Critically, transplanted rats rejected a second, nonengineered skin graft from the same donor species to the contralateral limb at a later date, demonstrating that recipient systemic immunocompetence remained intact. This study represents a novel approach in transplant immunology and highlights the significant therapeutic opportunity of the ex vivo period in transplant engineering.

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“…The transected donor and recipient muscle bellies, proximal to the musculotendinous junctions, were then approximated with interrupted 6‐0 Vicryl SUTURES (Ethicon, Inc., Sommerville, NJ). The vessels were anastomosed using a nonsuture cuff technique, as previously described (Figures C – E) . Donor vessel ends were everted over cuffs during the donor procedure to ensure direct endothelial contact at the anastomosis site.…”

Section: Methodsmentioning

confidence: 99%

A Novel Rodent Orthotopic Forelimb Transplantation Model That Allows for Reliable Assessment of Functional Recovery Resulting From Nerve Regeneration

Kern

Budihardjo

Mermulla

et al. 2017

American Journal of Transplantation

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Improved nerve regeneration and functional outcomes would greatly enhance the utility of vascularized composite allotransplantation (VCA) such as hand and upper extremity transplantation. However, research aimed at achieving this goal has been limited by the lack of a functional VCA animal model. We have developed a novel rat midhumeral forelimb transplant model that allows for the characterization of upper extremity functional recovery following transplantation. At the final end point of 12 weeks, we found that animals with forelimb transplantation including median, ulnar and radial nerve coaptation demonstrated significantly improved grip strength and forelimb function as compared to forelimb transplantation without nerve approximation (grip strength: 1.71N ± 0.57 vs. no appreciable recovery; IBB scale: 2.6 ± 0.7? vs. 0.8 ± 0.40; p = 0.0005), and similar recovery to nerve transection-and-repair only (grip strength: 1.71N ± 0.57 vs. 2.03 ± 0.42.6; IBB scale: 2.6 ± 0.7 vs. 2.8 ± 0.8; p = ns). Moreover, all forelimb transplant animals with nerve coaptation displayed robust axonal regeneration with myelination and reduced flexor muscle atrophy when compared to forelimb transplant animals without nerve coaptation. In conclusion, this is the first VCA small-animal model that allows for reliable and reproducible measurement of behavioral functional recovery in addition to histologic evaluation of nerve regeneration and graft reinnervation.

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A Rapid Vascular Anastomosis Technique for Hind-Limb Transplantation in Rats

Cited by 11 publications

References 9 publications

Adipose- and Bone Marrow–Derived Mesenchymal Stem Cells Prolong Graft Survival in Vascularized Composite Allotransplantation

Adipose- and Bone Marrow–Derived Mesenchymal Stem Cells Prolong Graft Survival in Vascularized Composite Allotransplantation

Ex vivo allotransplantation engineering: Delivery of mesenchymal stem cells prolongs rejection-free allograft survival

A Novel Rodent Orthotopic Forelimb Transplantation Model That Allows for Reliable Assessment of Functional Recovery Resulting From Nerve Regeneration

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