A rapid urine creatinine assay by capillary zone electrophoresis

Gatti, Rosalba; Lazzarotto, V; Palo, C. B. De; Cappellin, Enrico; Spinella, Paolo; Palo, Elio Franco De

doi:10.1002/(sici)1522-2683(19991001)20:14<2917::aid-elps2917>3.0.co;2-w

Cited by 38 publications

(10 citation statements)

References 29 publications

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“…Urinary creatinine (crea) was measured by a capillary electrophoresis method [20] and the urinary total protein (uTp) concentration was measured by the Coomassie method using a commercial kit purchased from Sigma Diagnostics (Sigma-Aldrich, St. Louis, MO, USA).…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

Erratum to “Urinary high performance reverse phase chromatography cortisol and cortisone analyses before and at the end of a race in elite cyclists”

Gatti¹,

Cappellin²,

Zecchin³

et al. 2005

Journal of Chromatography B

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A functional and basic method for the quantitative analysis of urine cortisol (F) and cortisone (E) using a solid-phase extraction (SPE) column and HPLC with ultraviolet detection is here described and validated to analyse urine samples. Urine specimens were analysed to study F and E relation and ratio in athletes and healthy sedentary subjects. The F and E concentrations in random urine specimens were significantly higher in the post-exercise versus pre exercise condition in cyclists (F: 136 ± 93 nmol/l versus 67 ± 50 nmol/l (p < 0.001); E: 797 ± 400 nmol/l versus 408 ± 252 nmol/l (p < 0.001)). The F/E ratio was 0.18 ± 0.11 versus 0.16 ± 0.07, respectively, and a significant difference was only demonstrated comparing sedentary (0.11 ± 0.07) and cyclist individuals at rest (p < 0.05). . was mistakenly published in uncorrected form in Volume 824, Issues 1-2, September 2005. It can be viewed by visiting the following link: http://dx.

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Section: Chemicals and Reagentsmentioning

confidence: 99%

Erratum to “Urinary high performance reverse phase chromatography cortisol and cortisone analyses before and at the end of a race in elite cyclists”

Gatti¹,

Cappellin²,

Zecchin³

et al. 2005

Journal of Chromatography B

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“…An alternative way of determining the creatinine content of such samples is to use HPLC-MS. We have developed a reliable HPLC-MS/MS method using an isotopically labeled standard for this purpose [15]. Another method reported to quantify creatinine in urine is the use of capillary zone electrophoresis [16], but these analyses are costly to perform. Therefore, we aimed to develop a fast and less expensive 1 H-NMR method for quantitative determination of creatinine in urine samples.…”

Section: Introductionmentioning

confidence: 99%

A <sup>1</sup>H-NMR based Method for Quantifying Creatinine in Urine Samples from Human Intervention Studies

Mueller¹,

Kelm²,

Ullrich³

et al. 2018

Eurasian J Anal Chem

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To overcome problems arising from the coloration of urine samples and the limitations of the picric acid method when determining creatinine in urine, we have established an alternative method using 1 H-NMR. The method is practically straight forward, provides good recoveries of 105 ± 2%, and a linear regression yielded an R² value of 1. The method revealed a similar precision to the standard procedure based on the Jaffé reaction for urine samples without interfering components.

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“…Numerous approaches for creatinine assay have been reported in the literature, such as enzymatic methods , strip assay , HPLC , MS , CZE , potentiometric biosensors , flow , and sequential injection analysis systems . Therefore, the development of an accurate and reliable method for routine creatinine measurements remains an important objective to improve the care of renal patient across the world .…”

Section: Introductionmentioning

confidence: 99%

Point of care creatinine measurement for diagnosis of renal disease using a disposable microchip

et al. 2013

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A point-of-care device for the determination of elevated creatinine levels in blood is reported. This device potentially offers a new and simple clinical regime for the determination of creatinine that will give huge time savings and removal of several steps of determination. The test employs a disposable prefilled microchip and the handheld Medimate Multireader®. By optimizing the analytical conditions it was found that the LOD of the proposed method was 87 μM creatinine, close to the normal human serum levels that are in the range of 60 to 100 μM. A statistical analysis of the residual shows a normal distribution, indicating the absence of systematic errors in the proposed method. The test can be used to distinguish patients with renal insufficiency (creatinine levels >100 μM) from healthy persons. Long-term monitoring could furthermore distinguish between acute renal failure and chronic kidney disease by the rate of creatinine concentration rise.

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A rapid urine creatinine assay by capillary zone electrophoresis

Cited by 38 publications

References 29 publications

Erratum to “Urinary high performance reverse phase chromatography cortisol and cortisone analyses before and at the end of a race in elite cyclists”

Erratum to “Urinary high performance reverse phase chromatography cortisol and cortisone analyses before and at the end of a race in elite cyclists”

A <sup>1</sup>H-NMR based Method for Quantifying Creatinine in Urine Samples from Human Intervention Studies

Point of care creatinine measurement for diagnosis of renal disease using a disposable microchip

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