A rapid, two-hour method for the enumeration of total viable bacteria in samples from commercial milk powder and whey protein concentrate powder manufacturing plants

Flint, Steve; Drocourt, Jean-Louis; Walker, Kylie; Stevenson, Barbara; Dwyer, Michelle; Clarke, Ian E.; Mcgill, D.

doi:10.1016/j.idairyj.2005.05.001

Cited by 38 publications

(25 citation statements)

References 5 publications

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“…This degree of correlation was not evident when FCM involved analysis of mixed strains in commercial products which may have been manufactured and stored under differing conditions. Further development and validation of an FCM based assay for total viable count enumeration of bacteria in milk and dairy products was reported by Flint et al (2006) using a commercial flow cytometry based system, D-Count from Chemunex S.A, Paris, France. The method involved adding a defined amount of sample (1 g) to a broth and incubating for 90 min at ∼63°C to enable outgrowth of thermophilic spores.…”

Section: Stains and Viabilitymentioning

confidence: 99%

Flow cytometry as a potential method of measuring bacterial viability in probiotic products: A review

Wilkinson

2018

Trends in Food Science & Technology

119

102

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Section: Stains and Viabilitymentioning

confidence: 99%

Flow cytometry as a potential method of measuring bacterial viability in probiotic products: A review

Wilkinson

2018

Trends in Food Science & Technology

119

102

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“…Recently, flow cytometry coupled with fluorescence techniques (to distinguish between cell types) has been used in a wide variety of biological assays in the milk dairy industry. 114,115 The microfluidic flow-cytometry systems have higher speed analytical capabilities, e.g., can count up to 25,000 cells per second, achieved by the cooperative use of microfluidics, optics and electronics ( Figure 12). 116 To this date, this microfluidic flow-cytometry system has been difficult to use for quantitative in vivo detection.…”

Section: Flow Cytometrymentioning

confidence: 99%

Applications of Microfluidic Devices in Food Engineering

2007

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The design of novel food micro-structures aimed at the quality, health and pleasure markets will probably require unit operations where the scale of the forming device is closer to the size of the structural elements (i.e., 1-100 μm). One emerging possibility is microfluidics or devices that employ small amounts of fluids (10 −6 to 10 −9 l) flowing in channels where at least one dimension is less than 1 mm. However, under these conditions, the predominant effects are not necessarily those present in conventional macroscopic unit operations. Dominant physical effects at the microfluidic scale are introduced through the use of dimensionless numbers. Different types of geometries to generate multi-phase flows in micro-channels, techniques and materials to construct the micro-devices, principally soft lithography and laser ablation, as well as methods used to modify surface properties of channels, are reviewed. The operation of micro-devices, the role of flow regimes, rheological behaviour of fluids in micro-channels and of transient time is discussed. Finally, systems developed to generate emulsions and foams, fluid mixing and dispersion, and future applications of these devices in food processing and food analysis are presented.

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“…FCM is a commonly used technique for quantifying bacteria in milk (Gunasekera et al, 2003;Holm et al, 2004), milk powder (Flint et al, 2006) and to monitor dairy starters during cheese ripening (Bunthof and Abee, 2002). Quantification of bacteria in these matrices are undertaken by several commercial instruments such as the BactoCount (Bentley Instruments Inc., Chaska, USA).…”

Section: Fcm Analysis Of Food Samplesmentioning

confidence: 99%

Application of Flow Cytometry to the Detection of Pathogenic Bacteria

Kennedy¹,

Wilkinson²

2017

Current Issues in Molecular Biology

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Outbreaks of infections have emphasized the necessity for rapid and economic detection methods for pathogens in samples ranging from those of clinical origin to food products during production and retail storage, and increasingly, in environmental samples. Flow cytometry (FCM) allows the rapid acquisition of multi-parametric data regarding cell populations within fluidised samples. However, the application of FCM to pathogen detection depends on the availability of specific fluorescent probes such as antibodies and RNA probes capable of detecting and isolating pathogens from these diverse samples. A particular issue for FCM methodology is the ability to recover and discriminate bacteria from the sample matrix which may pose a major technical hurdle towards accurate and sensitive analysis. This review article focuses on detection of pathogens using FCM in samples originating from food, water, environmental and clinical sources and outlines the current state of the art and potential future applications.

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A rapid, two-hour method for the enumeration of total viable bacteria in samples from commercial milk powder and whey protein concentrate powder manufacturing plants

Cited by 38 publications

References 5 publications

Flow cytometry as a potential method of measuring bacterial viability in probiotic products: A review

Flow cytometry as a potential method of measuring bacterial viability in probiotic products: A review

Applications of Microfluidic Devices in Food Engineering

Application of Flow Cytometry to the Detection of Pathogenic Bacteria

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