“…However, it could not identify monosaccharide constituents, linkages, or the anomeric character of the linkage (alpha versus beta). The nearly 200 collected fractions were further analyzed by rapid throughput monosaccharide and linkage analysis to provide nearly complete structures of each oligosaccharide component 54 – 57 . Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Each fraction was analyzed for monosaccharide compositions using a recently described rapid throughput automated workflow (Fig. 8b ) 54 , 57 . The linkage information was similarly obtained using a separate recently reported workflow for comprehensive linkage analysis 55 , 56 .…”
Section: Resultsmentioning
confidence: 99%
“…Monosaccharide analysis was adapted by Amicucci et al with the following modifications 54 . Briefly, fractionated oligosaccharides underwent acid hydrolysis with 4 M TFA for 2 h at 100 °C.…”
Polysaccharides are the most abundant biomolecules in nature, but are the least understood in terms of their chemical structures and biological functions. Polysaccharides cannot be simply sequenced because they are often highly branched and lack a uniform structure. Furthermore, large polymeric structures cannot be directly analyzed by mass spectrometry techniques, a problem that has been solved for polynucleotides and proteins. While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion. We describe the development and application of a chemical method for producing oligosaccharides from polysaccharides. The released oligosaccharides are characterized by advanced liquid chromatography–mass spectrometry (LC–MS) methods with high sensitivity, accuracy and throughput. The technique is first used to identify polysaccharides by oligosaccharide fingerprinting. Next, the polysaccharide compositions of food and feces are determined, further illustrating the utility of technique in food and clinical studies.
“…However, it could not identify monosaccharide constituents, linkages, or the anomeric character of the linkage (alpha versus beta). The nearly 200 collected fractions were further analyzed by rapid throughput monosaccharide and linkage analysis to provide nearly complete structures of each oligosaccharide component 54 – 57 . Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Each fraction was analyzed for monosaccharide compositions using a recently described rapid throughput automated workflow (Fig. 8b ) 54 , 57 . The linkage information was similarly obtained using a separate recently reported workflow for comprehensive linkage analysis 55 , 56 .…”
Section: Resultsmentioning
confidence: 99%
“…Monosaccharide analysis was adapted by Amicucci et al with the following modifications 54 . Briefly, fractionated oligosaccharides underwent acid hydrolysis with 4 M TFA for 2 h at 100 °C.…”
Polysaccharides are the most abundant biomolecules in nature, but are the least understood in terms of their chemical structures and biological functions. Polysaccharides cannot be simply sequenced because they are often highly branched and lack a uniform structure. Furthermore, large polymeric structures cannot be directly analyzed by mass spectrometry techniques, a problem that has been solved for polynucleotides and proteins. While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion. We describe the development and application of a chemical method for producing oligosaccharides from polysaccharides. The released oligosaccharides are characterized by advanced liquid chromatography–mass spectrometry (LC–MS) methods with high sensitivity, accuracy and throughput. The technique is first used to identify polysaccharides by oligosaccharide fingerprinting. Next, the polysaccharide compositions of food and feces are determined, further illustrating the utility of technique in food and clinical studies.
“…Methods presented here for workup were based on work by Xu et al . then refined for monosaccharide composition of polysaccharides by Amicucci et al . For total monosaccharide analysis, frozen brewed coffee samples (fractions 1–9 from Table , along with the whole brew sample) were vortexed and aliquoted in a 96‐well plate for hydrolysis.…”
Section: Methodsmentioning
confidence: 99%
“…Analysis was performed using the following procedures provided by Amicucci et al . Samples were analyzed on an Agilent 6495 triple quadrupole mass spectrometer coupled to an Agilent 1290 Infinity II ultrahigh‐performance liquid chromatograph.…”
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
