A Rapid Test for Beta-Lactamase Production by Hæmophilus Influenzæ

Slack, MaryP.E.; Wheldon, D. B.; Turk, D. C.

doi:10.1016/s0140-6736(77)90836-4

Cited by 30 publications

(11 citation statements)

References 10 publications

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“…In 1977, Slack et al reported a rapid test for Plactamase production by H. influenzae, using paper strips impregnated with penicillin and the pH indicator dye bromocresol purple (26). Later studies demonstrated that this method could detect resistance to 3-lactam antibiotics in Staphylococcus aureus, Neisseria gonorrhea, and certain Pseudomonas aeruginosa strains (25).…”

Section: Resultsmentioning

confidence: 99%

“…Later studies demonstrated that this method could detect resistance to 3-lactam antibiotics in Staphylococcus aureus, Neisseria gonorrhea, and certain Pseudomonas aeruginosa strains (25). The paper strip assay was designed to rapidly check small numbers of clinical isolates in the diagnostic laboratory (26). We report here a modified method in which bromocresol purple is used and that allows rapid screening of large numbers of E. coli colonies for the presence of a variety of 1-lactamases.…”

Section: Resultsmentioning

confidence: 99%

“…We describe an assay in which filter paper strips impregnated with benzylpenicillin and the pH indicator dye bromocresol purple are used and that allows screening of large numbers of Escherichia coli colonies for the presence of 3-lactamase. The assay, a modification of the method of Slack et al for detection of P-lactamase in limited numbers of clinical isolates of Haemophilus influenzae (26), has the combined advantages of speed, ease, minimal expense, continued viability of scored colonies, and applicability to a variety of ,B-lactamases.…”

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confidence: 99%

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Rapid Method for Screening Large Numbers of Escherichia coli Colonies for Production of Plasmid-Mediated β-Lactamases

Wolfson

Hooper

Swartz

et al. 1983

Antimicrob Agents Chemother

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Rapid Method for Screening Large Numbers of Escherichia coli Colonies for Production of Plasmid-Mediated β-Lactamases

Wolfson

Hooper

Swartz

et al. 1983

Antimicrob Agents Chemother

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“…A similar system using human red cells sensitised with rabbit anti-penicillinase antibodies was evaluated for the detection of penicillinase production. Results were compared with the acidometric method of detecting penicillinase production described by Slack et al (1977).…”

Section: Introductionmentioning

confidence: 99%

Reverse passive haemagglutination test for the rapid identification of Neisseria gonorrhoeae and detection of penicillinase production.

Munro¹,

Mallon²

1979

Sexually Transmitted Infections

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SUMMARY In a comparison of the reverse passive haemagglutination test (RPHA) with the direct immunofluorescent and rapid carbohydrate utilisation tests for the identification of Neisseria gonorrhoeae isolated from clinical specimens, 315 isolates of oxidase-positive Gram-negative diplococci were tested as pure 24-hour-old subcultures and samples from 108 similar organisms were taken directly from primary isolation plates. A similar test system was used to detect penicillinase production. Results showed agreement in 97 8Gbo of organisms tested with the RPHA and conventional methods for identification of N. gonorrhoeae; similarly there was good agreement with conventional methods for detection of penicillinase production. The test was reliable and could be read within four hours; a result was therefore available on the same day the clinical specimen was received. The time and work involved in identifying N. gonorrhoeae using the RPHA was less than with conventional methods, but differentiation between N. gonorrhoeae and other Neisseria species from throat swabs proved difficult.

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“…The use of an acidimetric paper method for the detection of 3-lactamase produced by Haemophilus influenzae and Neisseria gonorrhoea has been described (Slack et al, 1977). /3-lactamase producing strains of these organisms produce the enzyme in easily detectable amounts.…”

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confidence: 99%