A Rapid Technique for Determination of Races of <i>Phytophthora nicotianae</i> on Tobacco

Gutierrez, W. A.; Mila, A. L.

doi:10.1094/pdis-91-8-0985

Cited by 8 publications

(7 citation statements)

References 10 publications

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“…There is limited literature regarding P. parasitica spore preparation and plant infection in the laboratory or greenhouse. Methods vary depending on the planned experiments and include free chlamydospores, or hyphal mat applied to the stem wounds, or infected oat grains and leaf discs (39)(40)(41)(42). The method for spore preparation used here is very simple and inexpensive but requires individual application of spores to each potted plant in the greenhouse.…”

Section: Discussionmentioning

confidence: 99%

Use of Several Natural Products from Selected Nicotiana Species to Prevent Black Shank Disease in Tobacco

Kroumova

Artiouchine

Wagner

2016

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

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SUMMARY Black shank is a major annual disease threat to all types of tobacco worldwide. It is caused by the fungus Phytophthora parasitica var. nicotianae (PPN). The major tobacco growing areas in US - Kentucky, Tennessee and North Carolina can experience devastating losses, reaching in some fields up to 100%. Thus far, the main approaches to control this disease have been creation of resistant varieties, fungicide treatments, and crop rotation. Some fungicides are reported to have negative effects on the environment. The goal of this work was to test the antifungal activity of several natural products that are synthesized by certain Nicotiana species, and secreted to the leaf surface. We hypothesized that phylloplanin, cis-abienol, labdenediol and sclareol can suppress PPN-race 0- and PPN-race 1-caused disease in Burley tobaccos KY 14 and MS KY 14 × L8LC in the greenhouse. We developed methods for leaf surface extraction, spore preparation and soil drench application of the natural compounds tested. Experiments were performed on 5–8 week-old greenhouse grown seedlings. cis-Abienol showed high inhibitory properties toward the disease. Race 0 infection was completely subdued in KY 14 while race 1 infection was reduced by 70–80%, and delayed by 6–10 days in KY 14 and MS KY14 × L8LC. Sclareol was very effective in inhibiting race 0-caused disease in both tobacco cultivars. In MS KY 14 × L8LC race 1 infection was inhibited while in KY 14 it was reduced by 85% and delayed by 6 days. Labdenediol reduced the disease by half in eight week-old KY 14 plants. Tobacco phylloplanin reduced plant infection by both races by 50–60% and delayed the disease by 6–10 days. Phylloplanin was least suppressive in both tobacco cultivars. We consider sclareol to be the best candidate for future studies due to its antifungal properties and availability. cis-Abienol, despite its good antifungal activity, is not feasible for large-scale use due to the production and stability limitations.

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Section: Discussionmentioning

confidence: 99%

Use of Several Natural Products from Selected Nicotiana Species to Prevent Black Shank Disease in Tobacco

Kroumova

Artiouchine

Wagner

2016

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

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“…For example, in the early stage, the only available source of resistance was derived from Fla 301, which exhibited polygenic resistance. Dominant and monogenic resistance from Nicotiana plumbaginifolia Viv ( Php ) and N. longiflora Cav ( Phl ) was successfully incorporated into burley and flue-cured tobacco cultivars, respectively ( Gutiérrez and Mila, 2007 ; Antonopoulos et al, 2010 ). However, cultivars with known resistance resources have failed to provide sufficient control against the pathogen in the field.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and genetic characterization of resistance in Arabidopsis thaliana to the oomycete pathogen Phytophthora parasitica

et al. 2015

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The interaction between Arabidopsis thaliana and the oomycete pathogen Phytophthora parasitica emerges as a model for exploring the molecular basis and evolution of recognition and host defense. Phenotypic variation and genetic analysis is essential to dissect the underlying mechanisms in plant–oomycete interaction. In this study, the reaction phenotypes of 28 A. thaliana accessions to P. parasitica strain Pp016 were examined using detached leaf infection assay. The results showed the presence of four distinct groups based on host response and disease development. Of all the accessions examined, Zurich (Zu-1) is highly resistant to P. parasitica. Microscopic characterization showed that rapid and severe hypersensitive response at the primary infection epidermal cells is associated with disease resistance. Furthermore, Zu-1 is resistant to a set of 20 diverse P. parasitica strains, which were collected from different host plants and exhibited differential specificities on a set of tobacco cultivars. However, Zu-1 is susceptible to P. parasitica when the root is inoculated, suggesting differential expression of associated resistance genes in the root and foliar tissues. Genetic analysis by crossing Zu-1 and the susceptible accession Landsberg (Ler) showed that the resistance in Zu-1 to P. parasitica is semi-dominant, as shown by infection assays of F1 progenies, and is likely conferred by a single locus, defined as RPPA1Zu-1 (for Resistance to P. parasitica 1), as shown by analysis of F2 segregating populations. By employing specific-locus amplified fragment sequencing (SLAF-seq) strategy to identify molecular markers potentially linked to the locus, the strongest associated region was determined to be located between 7.1 and 11.2 Mb in chromosome IV. The future cloning of RPPA1Zu-1 locus will facilitate improved understanding of plant broad-spectrum disease resistance to oomycete pathogens.

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“…71 isolates from Colombian and a reference isolate from USA, were tested using the methodology proposed by and Gutiérrez & Mila (2007), but using tobacco differentials Hicks, KY 14xL8, L8 and NC 1071, which harbor different types of resistance to P. nicotianae (Table 1). Tobacco differentials were kindly donated by Dr. Ramses Lewis from North Carolina State University, USA.…”

Section: Determination Of Physiological Races Of Isolatesmentioning

confidence: 99%

“…Fla 301 is partially resistant to races 0 and 1, therefore is an option when both races of P. nicotianae are present (Apple, 1967;Lucas, 1975;Carlson et al, 1997;Csinos, 2005). Race 4 is able to overcome single-gene resistance conferred by the Phl gene from N. longiflora but not the Php gene from N. plumbaginifolia (Gutiérrez & Mila, 2007). In Colombia, most seeds used for commercial crops are imported from USA, where they are improved using cv Fla 301 (Johnson et al, 2002;Csinos, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Phytophthora nicotianae isolates from tobacco plants (Nicotiana tabacum) in Colombia

et al. 2022

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Characterization of Phytophthora nicotianae isolates from tobacco plants (Nicotiana tabacum) in ColombiaThe black shank disease caused by Phytophthora nicotianae causes losses in tobacco crops up 100%. In Colombia, P. nicotianae populations are poorly known causing wrong diagnostics and erratic management. Amplification of the Ypt1 gene and morphological characteristics of colonies, sporangia, chlamydospores and hyphae were used to identify P. nicotianae isolates. Races were identified according to the reaction induced by each isolate on the differential tobacco varieties Hicks, L8, KY 14 x L8 and NC 1071. As results, 71 isolates of P. nicotianae were identified and classified by races. Colonies of P. nicotianae were of white color, cottony and fluffy texture with smooth, non-swollen hyphae; spherical papillae with an average of 1.26 ìm and non-papillated and intercalary chlamydospores of medium size of 1.02 ìm that are typical characteristics of P. nicotianae. A species-specific PCR-amplified band of 389 bp was detected in all isolates tested. The presence of races 0,1 and 3 of P. nicotianae were determined in the Colombian departments of Huila and Santander. To the best of our knowledge, this is the first report of physiological races 0,1 and 3 of P. nicotianae in Colombia. Results are of relevance for disease management and tobacco breeding.

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A Rapid Technique for Determination of Races of Phytophthora nicotianae on Tobacco

Cited by 8 publications

References 10 publications

Use of Several Natural Products from Selected Nicotiana Species to Prevent Black Shank Disease in Tobacco

Use of Several Natural Products from Selected Nicotiana Species to Prevent Black Shank Disease in Tobacco

Phenotypic and genetic characterization of resistance in Arabidopsis thaliana to the oomycete pathogen Phytophthora parasitica

Characterization of Phytophthora nicotianae isolates from tobacco plants (Nicotiana tabacum) in Colombia

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