A rapid spectrophotometric procedure for the simultaneous micro determination of carbamazepine and 5,5-diphenyl-hydantoin in blood

Fellenberg, A. J.; Pollard, A.C.

doi:10.1016/0009-8981(76)90115-7

Cited by 7 publications

(2 citation statements)

References 9 publications

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“…Various methods have been reported for the determination of CBZ in pharmaceutical preparations including spectrophotometric methods [4][5][6][7][8], spectrofluorimetry method [9], gas-liquid chromatography (GC) [10,11], FT-Raman spectroscopy [12], planar chromatography [13], and high performance liquid chromatography (HPLC) [14][15][16][17][18][19][20][21][22][23][24]. Most of the methods reported are highly sophisticated, costly, and time consuming and require special sample preparation.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of HPTLC Method for Estimation of Carbamazepine in Formulations and Its In Vitro Release Study

Patel

Bhatt

et al. 2011

Chromatography Research International

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A new, simple, and rapid high-performance thin-layer chromatographic method was developed and validated for quantitative determination of Carbamazepine. Carbamazepine was chromatographed on silica gel 60F254TLC plate using ethyl acetate-toluene-methanol (5.0 + 4.0 + 1.0 v/v/v) as mobile phase. Carbamazepine was quantified by densitometric analysis at 285 nm. The method was found to give compact spots for the drug (Rf=0.47 ± 0.01). The linear regression analysis data for the calibration plots showed good linear relationship withr2=.9995in the concentration range 100–600 ng/spot. The method was validated for precision, recovery, repeatability, and robustness as per the International Conference on Harmonization guidelines. The minimum detectable amount was found to be 16.7 ng/spot, whereas the limit of quantitation was found to be 50.44 ng/spot. Statistical analysis of the data showed that the method is precise, accurate, reproducible, and selective for the analysis of Carbamazepine. The method was successfully employed for the estimation of equilibrium solubility, quantification of Carbamazepine as a bulk drug, in commercially available preparation, and in-house developed mucoadhesive microemulsion formulations and solution.

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Section: Introductionmentioning

confidence: 99%

Development and Validation of HPTLC Method for Estimation of Carbamazepine in Formulations and Its In Vitro Release Study

Patel

Bhatt

et al. 2011

Chromatography Research International

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“…Many of the initial studies on the therapeutic or toxic effects of these drugs were facilitated by the use of ultraviolet spectroscopy and thin layer chromatography (TLC). The former procedure is not very practical because it requires a large volume of sample, complex extraction techniques, is time consuming, and the assay is subjected to potential interference of other compounds (Fellenberg & Pollard 1976;Spiehler 1976;Kabra et al 1981). TLC, on the other hand, possesses adequate resolution for identifying many of the anticonvulsant drugs, but it is difficult to quantify these drugs accurately and precisely (Breyer & Villumsen 1975;Kabra et all981).…”

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confidence: 99%

Comparison of fluorescence polarization immunoassay and high performance liquid chromatography for the quantitative determination of phenytoin, phenobarbitone and carbamazepine in serum

Ashy

El–Sayed

Islam

1986

Journal of Pharmacy and Pharmacology

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The Abbott TDx fluorescence polarization immunoassay (FPIA) system has been evaluated and compared with well-established high performance liquid chromatography (HPLC) for the determination of three anticonvulsant drugs: phenytoin, phenobarbitone and carbamazepine. These assays were evaluated for precision, calibration curve stability, specificity and accuracy. Within-run precision studies using control samples (n = 15) in the subtherapeutic, therapeutic, and toxic concentrations, resulted in coefficients of variation in the range of 1.79-3.99% (FPIA) and 1.16-2.52% (HPLC), respectively. Between-run precision ranged from 2.32-6.34% for FPIA and from 2.04-3.38% for HPLC. Comparison of 122 patient samples assayed with both methods indicated an extremely good analytical correlation (r = 0.96) for all three comparisons. The FPIA method offers significant advantages in calibration curve stability while maintaining accuracy and precision comparable with those of established HPLC procedures.

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A rapid and sensitive gas-liquid chromatographic procedure for the micro determination of sodium valproate (sodium di-N-propylacetate) in plasma or serum

Fellenberg

Pollard

1977

Clinica Chimica Acta

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A rapid spectrophotometric procedure for the simultaneous micro determination of carbamazepine and 5,5-diphenyl-hydantoin in blood

Cited by 7 publications

References 9 publications

Development and Validation of HPTLC Method for Estimation of Carbamazepine in Formulations and Its In Vitro Release Study

Development and Validation of HPTLC Method for Estimation of Carbamazepine in Formulations and Its In Vitro Release Study

Comparison of fluorescence polarization immunoassay and high performance liquid chromatography for the quantitative determination of phenytoin, phenobarbitone and carbamazepine in serum

A rapid and sensitive gas-liquid chromatographic procedure for the micro determination of sodium valproate (sodium di-N-propylacetate) in plasma or serum

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