A Rapid Silver Staining and Destaining Technique for the Nucleolus Organizer Region

Abstract

Silver staining of nucleolar organizing regions (NOR) is common, but a standard protocol is lacking. A modification of a rapid silver nitrate staining technique for NORs is presented here. Advantages of the modified technique include reliability, speed, cost and the fact that it can be carried out in the light.

Arsenic-induced morphogenic response in roots of arsenic hyperaccumulator fern Pteris vittata

“…Five root tips for each treatment, carried out as described in the previous paragraph, were ethanol-acetic acid fixed, squashed in 45% acetic acid and air dried. The slides were subsequently stained with silver nitrate and incubated in a moist chamber at 37 • C for 20 min [16,17]. Then they were rinsed thoroughly in distilled water and air dried.…”

Effect of methotrexate and vitamin A on NOR expression in seminiferous tubules of Wistar rats

“…Rapid staining and de-staining method developed by Dhar, Kumar, Nayak et al (1995) was used in the present study.…”

“…This phase enables the cells to grow and produce all the necessary proteins for DNA synthesis. Cancer cells escape from the controls on cell division and the action mechanism of antineoplastic drug is based on the stopping of uncontrolled cells at any one stage of the cell cycle (DHAR, KUMAR, NAYAK et al, 1995). To further understand the relationship between the cell cycle and quantity of AgNOR proteins, it is necessary to determine the phases of cell cycle during which expression of AgNOR varies and it is important to know the most variable proteins in each phase (CROCKER, BOLDY and EGAN, 1989).…”