A rapid, sensitive screening test for polycyclic aromatic hydrocarbons applied to Antarctic water

Valero-Navarro, Ángel; Medina-Castillo, Antonio L.; Fernández-Ibáñez, Fermín; Segura‐Carretero, Antonio; Ibáñez, Jesús M.; Fernández‐Gutiérrez, Alberto

doi:10.1016/j.chemosphere.2006.11.028

Cited by 14 publications

(9 citation statements)

References 32 publications

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“…Another important finding concerns the high number of genes belonging to the catabolism of toxic chloro- and nitro-aromatic, aromatic, and alkanes compounds, some of which have been described as originating from anthropogenic activities (Kunze et al, 2009; Ju and Parales, 2010). Accordingly, Deception Island is one of the most visited zones of the Antarctic South Shetland archipelago (more than 20,000 people per year) and served as a trading point for more than two decades due to a whaling factory and tourism (Killingbeck, 1977; Valero-Navarro et al, 2007). In addition, there are several military bases where the use of hydrocarbons is constantly needed (Valero-Navarro et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Accordingly, Deception Island is one of the most visited zones of the Antarctic South Shetland archipelago (more than 20,000 people per year) and served as a trading point for more than two decades due to a whaling factory and tourism (Killingbeck, 1977; Valero-Navarro et al, 2007). In addition, there are several military bases where the use of hydrocarbons is constantly needed (Valero-Navarro et al, 2007). As the handling of fuels and hydrocarbons in Deception was not regulated until 2005, several spills may have occurred in the past.…”

Section: Discussionmentioning

confidence: 99%

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In-Depth Genomic and Phenotypic Characterization of the Antarctic Psychrotolerant Strain Pseudomonas sp. MPC6 Reveals Unique Metabolic Features, Plasticity, and Biotechnological Potential

et al. 2019

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We obtained the complete genome sequence of the psychrotolerant extremophile Pseudomonas sp. MPC6, a natural Polyhydroxyalkanoates (PHAs) producing bacterium able to rapidly grow at low temperatures. Genomic and phenotypic analyses allowed us to situate this isolate inside the Pseudomonas fluorescens phylogroup of pseudomonads as well as to reveal its metabolic versatility and plasticity. The isolate possesses the gene machinery for metabolizing a variety of toxic aromatic compounds such as toluene, phenol, chloroaromatics, and TNT. In addition, it can use both C6- and C5-carbon sugars like xylose and arabinose as carbon substrates, an uncommon feature for bacteria of this genus. Furthermore, Pseudomonas sp. MPC6 exhibits a high-copy number of genes encoding for enzymes involved in oxidative and cold-stress response that allows it to cope with high concentrations of heavy metals (As, Cd, Cu) and low temperatures, a finding that was further validated experimentally. We then assessed the growth performance of MPC6 on glycerol using a temperature range from 0 to 45°C, the latter temperature corresponding to the limit at which this Antarctic isolate was no longer able to propagate. On the other hand, the MPC6 genome comprised considerably less virulence and drug resistance factors as compared to pathogenic Pseudomonas strains, thus supporting its safety. Unexpectedly, we found five PHA synthases within the genome of MPC6, one of which clustered separately from the other four. This PHA synthase shared only 40% sequence identity at the amino acid level against the only PHA polymerase described for Pseudomonas (63-1 strain) able to produce copolymers of short- and medium-chain length PHAs. Batch cultures for PHA synthesis in Pseudomonas sp. MPC6 using sugars, decanoate, ethylene glycol, and organic acids as carbon substrates result in biopolymers with different monomer compositions. This indicates that the PHA synthases play a critical role in defining not only the final chemical structure of the biosynthesized PHA, but also the employed biosynthetic pathways. Based on the results obtained, we conclude that Pseudomonas sp. MPC6 can be exploited as a bioremediator and biopolymer factory, as well as a model strain to unveil molecular mechanisms behind adaptation to cold and extreme environments.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In-Depth Genomic and Phenotypic Characterization of the Antarctic Psychrotolerant Strain Pseudomonas sp. MPC6 Reveals Unique Metabolic Features, Plasticity, and Biotechnological Potential

et al. 2019

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“…Benzo(a)pyrene (BaP), one of the most toxic PAHs, is usually selected as an indicator of the presence of other compounds belonging to that group [11]. It has been detected in a diversity of aqueous matrices such as surface waters, seawaters, groundwater, drinking water, as well as in sediments [12].…”

Section: Introductionmentioning

confidence: 99%

Preliminary Feasibility Study of Benzo(a)Pyrene Oxidative Degradation by Fenton Treatment

Homem

Dias

Santos

et al. 2009

Journal of Environmental and Public Health

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Polycyclic aromatic hydrocarbons (PAHs) are considered priority compounds due to their toxic and carcinogenic nature. The concern about water contamination and the consequent human exposure has encouraged the development of new methods for PAHs removal. The purpose of this work was to study the feasibility of a degradation process of benzo(a)pyrene (BaP) in aqueous matrices by oxidation with Fenton reagent. A laboratory unit was designed to optimize the factors which may influence the process: pH (3.5 to 6.0), temperature (30 to 70°C), H2O2 (20 to 150 mg L−1), Fe2+ concentration (2.75 to 5.50 mg L−1), and the initial concentration of the pollutant (10 to 100 μg L−1). The pH did not influence significantly the results in the range studied. An increase in temperature from 30 to 70°C improved the removal efficiency from 90% to 100%. The same effect was observed for ferrous ion concentrations from 2.75 to 5.50 mg L−1 (increase from 78% to 100% removal). The H2O2 concentration played a double role during the process: from 20 to 50 mg L−1 an increase in the removal efficiency was achieved, but for higher concentrations (>50 mg L−1) the degradation is lower. This study proved that the degradation of benzo(a)pyrene by Fenton's reagent is a viable process.

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“…However, if the emission spectra (or the absorption spectra) of the analyte are partly overlapped with those of the co-existent organic compounds, a separation method of the analyte, a multi-component analytical method [82] or a statistical treatment of the analytical data [12,34] with spectroscopic detection [78,79]. The systems were controlled for automatic analysis by personal computers.…”

Section: Organic Compoundsmentioning

confidence: 99%

Recent trends in solid phase spectrometry: 2003–2009. A Review

Matsuoka

Yoshimura

2010

Analytica Chimica Acta

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Around 100 papers published from 2003 to the present are reviewed concerning analytical methods for the direct light measurement of a solid phase in which a target colored or fluorescent analyte is concentrated. Recent attention has been paid to the development of flow injection-solid phase spectrometry as a simple and inexpensive tool for routine analysis of organic compounds or pharmaceuticals. Due to some improvements in flow injection analysis, such as sequential injection and lab-on-a-valve, it is possible not only to reduce the reagent consumption but also to devise fully automatic and miniaturized systems with minimal maintenance needs. This may have the potential of becoming one of the green analytical methods. Flow injection-solid phase spectrometry is expected to be applied to the speciation of trace chemical components (e.g., specific determination of trace metal ions in different existent oxidation states) in real samples in the environment.

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A rapid, sensitive screening test for polycyclic aromatic hydrocarbons applied to Antarctic water

Cited by 14 publications

References 32 publications

In-Depth Genomic and Phenotypic Characterization of the Antarctic Psychrotolerant Strain Pseudomonas sp. MPC6 Reveals Unique Metabolic Features, Plasticity, and Biotechnological Potential

In-Depth Genomic and Phenotypic Characterization of the Antarctic Psychrotolerant Strain Pseudomonas sp. MPC6 Reveals Unique Metabolic Features, Plasticity, and Biotechnological Potential

Preliminary Feasibility Study of Benzo(a)Pyrene Oxidative Degradation by Fenton Treatment

Recent trends in solid phase spectrometry: 2003–2009. A Review

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