A Rapid Radioimmunoassay for the Measurement of Antibodies to Glutamic Acid Decarboxylase in Human Serum

Abstract

Intra-assay and inter-assay variations are shown in Table 1. Intra-assay variation was performed on 4 IDDM samples with varying concentrations of GAD-Ab in 4 separate assays. Each sample was assayed in 12 duplicate tubes. Intra-assay percent coefficient of variation (%CV) ranged from 5.2 to 7% (6.1 0.3%; mean±SE). Inter-assay variation was determined in 6 duplicate tubes in 5 separate assays from 5 IDDM serum samples containing a wide range concentrations of GAD-Ab.